The systemic administration of neural stem cells expressing an inducible and soluble form of growth arrest specific 1 inhibits mammary gland tumor growth and the formation of metastases

Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways

“…#M0303S) and converted to cDNA using MMLV reverse transcriptase (Invitrogen, cat. #28025013) to perform the PCR [ 45 ]. To amplify AKT mRNA, the following primers were used: forward 5′-GCCATGAAGATCCTCAAGAAGG-3′ and reverse 5′-CATCATCTCGTACATGACCACG-3′.…”

Treating Metastatic Brain Cancers With Stem Cells

“…NSC carrying therapeutic suicide genes, such as CE and CD, target tumor sites to activate chemotherapy drugs and induce cell death ( Seol et al, 2011 ). Therapeutic use of allogenic human bone marrow-derived NSCs transfected with interferon-β (IFN-β) diminishes tumor growth, decreases metastasis, and increases longevity via AKT and ERK1/2 phosphorylation in the tumor cells ( Bao et al, 2012 ; Hagenhoff et al, 2016 ; Romero-Trejo et al, 2021 ). Combination therapy of IFN-β/MSCs advantageously does not require immunosuppression and directly controls melanoma proliferation, allowing it to function as an anti-tumor therapy ( Studeny et al, 2002 ; Du et al, 2019 ; Heo et al, 2019 ; Serakinci and Cagsin, 2019 ; Serhal et al, 2019 ).…”