The SYRMEP Beamline of Elettra: Clinical Mammography and Bio-medical Applications

“…Furthermore, a treated scaffold was imaged at the SYRMEP beamline of Elettra [24] by phase-contrast synchrotron X-ray µCT ( Figure 5) reaching a spatial resolution of the order of 1 µm. Figure 5 panel (a) shows a reconstructed tomographic slice with a somewhat reduced spatial resolution that highlights the presence of mineral depositions along brighter filament indicating a not random pattern.…”

Section: Depositions Characterization By X-ray Microtomography Technimentioning

confidence: 99%

“…One tomographic data set for a treated sample has been acquired at the SYRMEP beamline of Elettra [24], which was operated at an electron energy of 2.0 GeV. The source of the synchrotron radiation is a bending magnet featuring a magnetic field of 1.2 T, which generates a white spectrum of synchrotron radiation with a critical energy of about 3.2 KeV.…”

Section: Synchrotron-based X-ray Computed Microtomography Analysismentioning

confidence: 99%

Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells

Picone

Cappadone

Pasini

et al. 2020

IJMS

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In this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular magnesium content was found, both at three and seven days from the induction of differentiation. By X-ray microscopy, we characterized the morphology and chemical composition of the mineral depositions secreted by 3D cultured differentiated cells finding a marked co-localization of Mg with P at seven days of differentiation. This is the first experimental evidence on the presence of Mg in the mineral depositions generated during biomineralization, suggesting that Mg incorporation occurs during the bone forming process. In conclusion, this study on the one hand attests to an evident involvement of Mg in the process of cell differentiation, and, on the other hand, indicates that its multifaceted role needs further investigation.

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“…Synchrotron X-ray lCT measurements Region of interest (Tafforeau et al 2006;Rigon et al 2010) synchrotron radiation lCT scans were recorded for seven T. sexlineatus specimens at the SYRMEP beamline of the Elettra synchrotron facility (Tromba et al 2010). We used a filtered polychromatic X-ray beam with a mean energy of 22 keV.…”

Section: X-ray Computed Lctmentioning

confidence: 99%

Enhanced contrast in X‐ray microtomographic images of the membranous labyrinth using different X‐ray sources and scanning modes

Goyens

Vasilopoulou‐Kampitsi

Claes

et al. 2018

Journal of Anatomy

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The vestibular system, located in the inner ear, plays a crucial role in balance and gaze stabilisation by sensing head movements. The interconnected tubes with membranous walls of the vestibular system are located in the skull bone (the ‘membranous labyrinth’). Unfortunately, these membranes are very hard to visualise using three‐dimensional (3D) X‐ray imaging techniques. This difficulty arises due to the embedment of the membranes in the dense skull bone, the thinness of the membranes, and the small difference in X‐ray absorption between the membranes and the surrounding fluid. In this study, we compared the visualisation of very small specimens (lizard heads with vestibular systems smaller than 3 mm) by X‐ray computed micro‐tomography (μCT) based on synchrotron radiation and conventional sources. A visualisation protocol using conventional X‐ray μCT would be very useful thanks to the ease of access and lower cost. Careful optimisation of the acquisition parameters enables detection of the membranes by using μCT scanners based on conventional microfocus sources, but in some cases a low contrast‐to‐noise ratio (CNR) prevents fast and reliable segmentation of the membranes. Synchrotron radiation μCT proved to be preferable for the visualisation of the small samples with very thin membranes, because of their high demands for spatial and contrast resolution. The best contrast was obtained by using synchrotron radiation μCT working in phase‐contrast mode, leading to up to twice as high CNRs than the best conventional μCT results. The CNR of the synchrotron radiation μCT scans was sufficiently high enough to enable the construction of a 3D model by the means of semi‐automatic segmentation of the membranous labyrinth. Membrane thickness was found to range between 2.7 and 36.3 μm. Hence, the minimal membrane thickness was found to be much smaller than described previously in the literature (between 10 and 50 μm).

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The SYRMEP Beamline of Elettra: Clinical Mammography and Bio-medical Applications

Cited by 94 publications

References 10 publications

Using synchrotron X-ray microtomography to characterize the pore network of reservoir rocks: A case study on carbonates

Using synchrotron X-ray microtomography to characterize the pore network of reservoir rocks: A case study on carbonates

Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells

Enhanced contrast in X‐ray microtomographic images of the membranous labyrinth using different X‐ray sources and scanning modes

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