The Syrian golden hamster strain LPN: a useful animal model for human cholelithiasis

Combettes-Souverain, M.; Souidi, Maâmar; Parquet, Michel; Férézou, Jacqueline; Riottot, Michel; Sérougne, Colette; Lutton, C.

doi:10.1016/s0955-2863(01)00217-0

Cited by 7 publications

(4 citation statements)

References 65 publications

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“…The metabolism of cholesterol in the hamster is similar to humans (Khallou et al, 1991b). The nature and composition of hydrophobic bile salts and the ratio of taurine-and glycine-conjugated bile salts are similar in hamsters and humans ( Figure 34.1) (Combettes-Souverain et al, 2002;Hardison, 1983;Singhal et al, 1983). In earlier studies, gallstones were induced in Syrian hamsters by feeding a fat-free glucose-rich diet (Dam, 1971).…”

Section: Cholesterol Gallstonesmentioning

confidence: 83%

“…Male LPN hamsters (bred at Laboratoire de Physiologie et de la Nutrition, France) are highly susceptible to cholesterol gallstones (~70%) induced by a lowfat high-sucrose diet, whereas commercial Janvier (JAN) hamsters on a similar diet do not develop gallstones (Boehler et al, 1999;Combettes-Souverain et al, 2002;Ferezou et al, 2000). In LPN hamsters, the sucrose-rich lithogenic diet causes an increase in biliary cholesterol and phospholipid.…”

Section: Cholesterol Gallstonesmentioning

confidence: 99%

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The Experimental Use of Syrian Hamsters

Valentine

Daugherity

Singh

et al. 2012

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents

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Section: Cholesterol Gallstonesmentioning

confidence: 83%

Section: Cholesterol Gallstonesmentioning

confidence: 99%

The Experimental Use of Syrian Hamsters

Valentine

Daugherity

Singh

et al. 2012

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents

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“…Details of diet formulation and composition are published elsewhere (16). Syrian hamsters were used in this study because of their well documented similarities to human cholesterol and bile acid metabolism (19). All experimental procedures were approved by the Institutional Animal Care and Use Committee at the University of Nebraska.…”

Section: Animals and Dietsmentioning

confidence: 99%

Dietary Stearic Acid Alters Gallbladder Bile Acid Composition in Hamsters Fed Cereal-Based Diets

Cowles

Lee

Gallaher

et al. 2002

The Journal of Nutrition

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Stearic acid (18:0) is unique among the saturated fatty acids commonly found in the food supply because, unlike palmitic, myristic and lauric acid, it does not raise plasma cholesterol when consumed (1-3). Studies in rats (4 -6) and hamsters (7,8) have indicated that dietary 18:0 also reduces liver cholesterol concentration. The hypocholesterolemic effect of dietary 18:0 is related to a reduction in intestinal cholesterol absorption (9 -12) and increased cholesterol excretion from the body (4,7,13). Nevertheless, it is still unclear exactly how 18:0 alters cholesterol absorption.Our present hypothesis is that dietary 18:0 reduces cholesterol absorption by altering hepatic bile acid synthesis and gallbladder bile acid composition. Because intestinal absorption of cholesterol requires secretion of bile acids and formation of mixed micelles, the efficiency of cholesterol solubilization, and therefore absorption, depends in part on the relative hydrophobicity of the bile acids present in bile. Using relative hydrophobicity values established for individual bile acids (14), a "hydrophobicity index" was developed to describe the overall hydrophilic-hydrophobic balance of a bile acid mixture (15). Studies in rats (6) and hamsters (8) have shown that dietary 18:0 alters fecal bile acid composition, suggesting that 18:0 could reduce intestinal cholesterol absorption by decreasing the proportion of hydrophobic bile acids secreted into the small intestine.This work is an extension of a previous study in which we reported significantly reduced cholesterol absorption and increased cholesterol excretion in hamsters fed 18:0-enriched diets (16). A unique aspect of the study design is that hamsters were fed modified NIH-07 open formula diets that differed in only a single fatty acid, i.e., 18:0, palmitic acid (16:0), trans fatty acids (18:1t), oleic acid (18:1c) or linoleic acid (18:2). This design eliminated the confounding variable of multiple fatty acid difference among treatments and allowed us to isolate the metabolic effects of the specific fatty acids. MATERIALS AND METHODS Animals and diets. Male Syrian hamsters (ϳ30 d old; CharlesRiver, Wilmington, MA) weighing ϳ70 g were housed individually in polycarbonate cages with sawdust bedding. Hamsters were kept in an environmentally controlled room at 25°C with a 12-h light:dark cycle. They were fed a modified version of the NIH-07 open formula, cereal-based rodent diet (17,18). Each diet provided (g/100 g) 17 total fat, 24 protein, 40 carbohydrate, 4 crude fiber and 0.05 cholesterol. Details of diet formulation and composition are published elsewhere (16). Syrian hamsters were used in this study because of their well documented similarities to human cholesterol and bile acid metabolism (19). All experimental procedures were approved by the Institutional Animal Care and Use Committee at the University of Nebraska.Experimental design. Hamsters (n ϭ 64) were randomly divided into five groups. Each group contained 13 hamsters (the 18:0 group contained 12 hamsters). Hamsters were...

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“…The Brazilian squirrel monkey, which is highly susceptible to spontaneous gallstone formation when fed atherogenic diets, has been used successfully as a model in cholelithiasis studies (Osuga and Portman, 1971;Tanaka et al, 1976). Meanwhile, these historical model animals have been replaced by rodent models such as the Syrian hamster and mouse models (Combettes-Souverain et al, 2002;Wang and Lee, 2008). However, cholelithiasis and its associated disorders appear to be less common in primates compared to humans (Smith et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

A case of gallstones in an African green monkey (Chlorocebus aethiops)

Kleinlützum

Plesker

2017

Primate Biol.

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Abstract. Spontaneous cholelithiasis was found in a male African green monkey (Chlorocebus aethiops) at necropsy. Choleliths varied in size, shape and colour. Gallstones were analysed using accepted analytical methods. Results showed that the gallstones were composed of cholesterol and protein in varying proportions. Histologically, the gallbladder showed diffuse mild to moderate lymphocytic infiltration. The etiology of the cholelithiasis in the examined individual remains unknown.

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The Syrian golden hamster strain LPN: a useful animal model for human cholelithiasis

Cited by 7 publications

References 65 publications

The Experimental Use of Syrian Hamsters

The Experimental Use of Syrian Hamsters

Dietary Stearic Acid Alters Gallbladder Bile Acid Composition in Hamsters Fed Cereal-Based Diets

A case of gallstones in an African green monkey (Chlorocebus aethiops)

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