The synthesis of porphyrins and bacteriochlorophyll in cell suspensions of <i>Rhodopseudomonas spheroides</i>

Lascelles, June

doi:10.1042/bj0620078

Cited by 301 publications

(148 citation statements)

References 46 publications

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“…Omission of iron from the medium increased the yield of porphyrin (cf. Lascelles, 1956) which was also greater when the basal medium was supplemented with casein hydrolysate (Table 3). The degree of aeration greatly influenced porphyrin production ( Table 3).…”

Section: J G Morrismentioning

confidence: 94%

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Studies on the Metabolism of Arthrobacter globiformis

Morris

1960

Journal of General Microbiology

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SUMMARY: A strain of Arthrobacter globiformis grew on a glucose + ammonium + salts medium (pH 7) when this was supplemented with biotin ( l O -e~) . Maximal and most rapid growth was obtained by aerobic incubation in shaken culture at 30". A variety of sugars, sugar alcohols, dicarboxylic acids, some amino acids and miscellaneous compounds could replace the glucose in this medium, but various aromatic compounds, purines, pyrimidines and other amino acids did not support growth, though several of these substances were oxidized by washed suspensions. The organism was an obligate aerobe whose terminal electron transport was mediated by a cytochrome system. Enzymic analysis showed that glucose could be metabolized by the hexose monophosphate oxidation and Embden-Meyerhof pathways. No direct oxidation of glucose to gluconic and 2-oxogluconic acids, and no Entner-Doudoroff pathway for the utilization of 6-phosphogluconate were demonstrable. Studies with differently [L4C]-labelled samples of glucose confirmed these findings and showed that in suspensions of organisms depleted of endogenous metabolites, the EmbdenMeyerhof pathway was fully functional, accounting for c. 65 yo of glucose utilization, the remaining 35% proceeding by way of the hexose monophosphate cycle. The pyruvate so formed was normally further oxidized by the tricarboxylic acid cycle. The growth yield of A . globifomis on limiting amounts of glucose was no greater than that of Escherichia coli under the same condition. These observations are considered in relation to the allocation of A. globifomis in the autochthonous group of soil micro-organisms.

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Section: J G Morrismentioning

confidence: 94%

“…Assay of inorganic phosphate was performed by the method of Berenblum & Chain (1988). Porphyrins were measured spectrophotometrically in acid solution (Lascelles, 1956). Protein was determined by the FolinCiocalteau procedure (Lowry, Rosebrough, Farr & Randall, 1951).…”

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confidence: 99%

Studies on the Metabolism of Arthrobacter globiformis

Morris

1960

Journal of General Microbiology

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“…Anaerobic, photoheterotrophic grovth of Rhodobacter sphaeroides (formerly, Rhodopseudomonas sphaeroides) ATCC 550 was carried out on medium S described by Lascelles (1956), and of Rhodocyclus gelatinosa (formerly, Rhodopseudomonas gelatinosa) ATCC 17011 on ATCC medium 112, the media being depleted of glutathione in both cases. Chlamydomonas reinhardtii 137Cm+ was kindly supplied by S. Howell and was grown photoautotrophically on the high salt medium (HSM) of Sueoka et al (1967).…”

Section: Methodsmentioning

confidence: 99%

The evolution of glutathione metabolism in phototrophic microorganisms

1987

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“…Nevertheless, it does show certain similarities with the biosynthetic activity of isolated Euglena chloroplasts, unicellular algae, and photosynthetic bacteria (3,4,6,10,16,30), suggesting that the same biochemical pathway for the synthesis of chlorophyll precursors which has been found in lower organisms is operating also in higher plants.…”

Section: Resultsmentioning

confidence: 70%

“…Rhodopseudomonas spheroides incubated with various substrates accumulate a major fraction of coproporphyrin m; the latter is accompanied sometimes by varying amounts of uroporphyrins I and II (6,10,16). Rhodopseudomonas capsulata, incubated in the light with a mixture containing glycine and succinate, synthesizes large amounts of bacteriochlorophyll and two intermediates: coproporphyrinogen III and protoporphyrin monomethyl ester (4).…”

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confidence: 99%

Porphyrin Biosynthesis in Cell-free Homogenates from Higher Plants

et al. 1970

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The porphyrin and phorbin biosynthetic activity of etiolated cucumber (Cucumis sativus, L.) cotyledons was compared to that of cotyledonary homogenates. Etiolated The terminology proposed by Kirk and Tilney-Basset is followed in this paper: the term "protochlorophyllide ester" denotes protochlorophyllide phytyl ester, while the term "protochlorophyll" denotes the mixture of protochlorophyllide and protochlorophyllide phytyl ester which accumulates in etiolated tissues.

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The synthesis of porphyrins and bacteriochlorophyll in cell suspensions of Rhodopseudomonas spheroides

Abstract: Free porphyrins are formed by growing cultures of some bacteria and yeasts under conditions in which formation of cytochromes is limited. With Vol. 62.

Cited by 301 publications

References 46 publications

Studies on the Metabolism of Arthrobacter globiformis

Studies on the Metabolism of Arthrobacter globiformis

The evolution of glutathione metabolism in phototrophic microorganisms

Porphyrin Biosynthesis in Cell-free Homogenates from Higher Plants

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