The synthesis of oligonucleotide-polyamide conjugate molecules suitable as PCR primers

DNA Meets Synthetic Polymers — Highly Versatile Hybrid Materials

“…A first approach towards using DNA block copolymers as probes for DNA detection was undertaken by Haralambidis and co-workers. 78 The rationalization behind the use of employing a block copolymer architecture was that the nucleic acid part is needed for molecular recognition, while the polymer block allows the incorporation of multiple labels along the backbone. A synthetically challenging method was developed for achieving the linkage between the ODN and the organic polymer segment, since the polyamide was attached to the base of the nucleotide at the 5 -end.…”

“…A synthetically challenging method was developed for achieving the linkage between the ODN and the organic polymer segment, since the polyamide was attached to the base of the nucleotide at the 5 -end. 78 The polyamide unit was synthesized employing standard Fmoc chemistry. This allowed the…”

Drug delivery systems based on nucleic acid nanostructures

“…More recently, several groups realized such micellar morphologies and in addition showed that the overall structure can be altered from spherical to cylindrical assemblies by stimuli like changes in pH, addition of endonucleases or through hybridization with complementary DNA [33,34]. Due to these unique properties, DBCs are currently applied in purification of biomaterials [35,36], DNA detection [37], templated synthesis [38] and in nanoelectronics [39].…”

Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases

“…The synthesis of FmocAhx-d 8 7 (Scheme 1) starts with a copper(I) catalyzed oxidative Glaser coupling 16 of Fmoc-protected propargylamine 17 (3) and benzyl propiolate 18 (4) to furnish diyne 5. Upon reduction of diyne 5 with deuterium gas and palladium on carbon, the benzyl ester remained intact, yielding deuterated 6.…”