The Synthesis of Novel Aminoacid Based Amides and Evaluation of Anticancer Effects on Hela Cervical Cancer Cells

Abstract

In this study, lysine, phenylalanine, histidine, valine and proline amino acids were reacted with pnitrobenzoyl chloride to give amide derivative compounds. The structure of these compounds was elucidated by FT-IR. The cytotoxic, apoptotic and angiogenic effects of these synthesized compounds were studied in vitro on the HeLa cervical cancer cell line. The cytotoxic activities of 25, 50, 100 and 200 μM doses of the synthesized compounds on HeLa cells were determined spectrophotometrically using the WST-I method. B1, B2 and B5 showed cytotoxic effect on HeLa cells whereas B3 and B4 compounds induced proliferation. The amount of poly (ADP-Ribose) Polymerase-I (PARP-I) and vascular endothelial growth factor (VEGF) molecules were measured spectrophotometrically by ELISA method. The PARP-I levels all compounds at all doses were statistically decreased compared to the control group (p <0.05). The VEGF levels B1, B2 and B5 compounds statistically decreased all doses compared to control whereas 200 µm dose of the B4 compound increased compared to the control group