The synthesis of allylic phosphate derivatives of trans zeatin

Formation of zeatin allylic phosphate by the microsomal fractions of bulb disks of Iris × hollandica Tub. and tubers of Helianthus tuberosus L.

“…However, chemical synthesis of ZAP [14] made it possible to compare the properties of this synthetic compound with those obtained from the bound cytokinins extracted from bulb disks . Indeed, this synthetic compound was retained in the water layer after partition with water-saturated butan-l-ol (data not shown) .…”

“…Zeatin-allylic-phosphate (ZAP) was synthesized by Shadid et al . [14] . 8-[14 C]-Zeatin (specific act .…”

“…This compound was synthesized by Shadid et al . [14] . We tested its cross-reactivity with antibodies raised against ZR and found it to be not cross-reactive (ZR : 50% Bo = 3 pmol, ZAP : 50% Bo = > 10 .000 pmol).…”

“…Another sample of microsomal proteins was purified by HAUltrogel separation to demonstrate the relation between ZAP formation and the concentration of protein (fraction no . [11][12][13][14] . A linear correlation with a correlation coefficient of 0 .997 between amounts of 14 C-ZAP formed and protein concentration was obtained (Figure 3) .…”

“…A linear correlation with a correlation coefficient of 0 .997 between amounts of 14 C-ZAP formed and protein concentration was obtained (Figure 3) . Formation of ZAP by purified microsomal proteins (fraction numbers [11][12][13][14] incubated with AMP and [14C]-zeatin for different time periods is shown in Figure 4 . From these results we concluded that ZAP was formed by an enzyme reaction .…”