The Synergistic Effect of Low-Dose Cyclosporine and Fluocinolone Acetonide on the Survival of Rat Allogeneic Skin Grafts

Xf, Zhao; Jw, Alexander; Schroeder, Timothy; Gf, Babcock

doi:10.1097/00007890-198810000-00003

Cited by 8 publications

(3 citation statements)

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“…8,9 MicroRNAs (miRNAs) have emerged as a class of small singlestranded noncoding RNAs that are approximately 22 nt in length. [10][11][12] It is well-known that miRNAs regulate some pathways involved in the pathological conditions through binding to the 3ʹ UTR of the target genes. [13][14][15] Recently, several miRNAs have been found to be associated with the differentiation of stem cells.…”

Section: Introductionmentioning

confidence: 99%

miR-149-3p Regulates the Switch between Adipogenic and Osteogenic Differentiation of BMSCs by Targeting FTO

Yang

Gao

et al. 2019

Molecular Therapy - Nucleic Acids

117

100

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Bone marrow-derived mesenchymal stem cells (BMSCs) have been suggested to possess the capacity to differentiate into different cell lineages. Maintaining a balanced stem cell differentiation program is crucial to the bone microenvironment and bone development. MicroRNAs (miRNAs) have played a critical role in regulating the differentiation of BMSCs into particular lineage. However, the role of miR-149-3p in the adipogenic and osteogenic differentiation of BMSCs has not been extensively discovered. In this study, we aimed to detect the expression levels of miR-149-3p during the differentiation of BMSCs and investigate whether miR-149-3p participated in the lineage choice of BMSCs or not. Compared with mimic-negative control (NC), miR-149-3p mimic decreased the adipogenic differentiation potential of BMSCs and increased the osteogenic differentiation potential. Further analysis revealed that overexpression of miR-149-3p repressed the expression of fat mass and obesity-associated (FTO) gene through binding to the 3ʹ UTR of the FTO mRNA. Also, the role of miR-149-3p mimic in inhibiting adipogenic lineage differentiation and potentiating osteogenic lineage differentiation was mainly through targeting FTO, which also played an important role in regulating body weight and fat mass. In addition, BMSCs treated with miR-149-3p anti-miRNA oligonucleotide (AMO) exhibited higher potential to differentiate into adipocytes and lower tendency to differentiate into osteoblasts compared with BMSCs transfected with NC. In summary, our results detected the effects of miR-149-3p in cell fate specification of BMSCs and revealed that miR-149-3p inhibited the adipogenic differentiation of BMSCs via a miR-149-3p/FTO regulatory axis. This study provided cellular and molecular insights into the observation that miR-149-3p was a prospective candidate gene for BMSC-based bone tissue engineering in treating osteoporosis.

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Section: Introductionmentioning

confidence: 99%

miR-149-3p Regulates the Switch between Adipogenic and Osteogenic Differentiation of BMSCs by Targeting FTO

Yang

Gao

et al. 2019

Molecular Therapy - Nucleic Acids

117

100

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“…In general, the rationale for topical delivery of calcineurin inhibitors and steroidal anti-inflammatory drugs are weakened by their lipophilicity and penetration into the vasculature of deeper tissues. Furthermore, Zhao and colleagues have reported bacterial infiltrates in skin allografts of rats received topical cyclosporine A and fluocinolone acetonide [35]. Thus topical or local drug delivery per se does not necessarily circumvent the toxicities of systemic immunosuppressants.…”

Section: Discussionmentioning

confidence: 99%

Antibody-functionalized peptidic membranes for neutralization of allogeneic skin antigen-presenting cells

Wen

Bagia

et al. 2014

Acta Biomaterialia

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We report herein application of an in situ material strategy to attenuate allograft T cell responses in a skin transplant mouse model. Functionalized peptidic membranes were used to impede trafficking of donor antigen-presenting cells (dAPCs) from skin allografts in recipient mice. Membranes formed by self-assembling peptides (SAPs) presenting antibodies were found to remain underneath grafted skins for up to 6 days. At the host-graft interface, dAPCs were targeted by using a monoclonal antibody that binds to a class II MHC molecule (IAd) expressed exclusively by donor cells. Using a novel cell labeling near-infrared nanoemulsion, we found more dAPCs remained in allografts treated with membranes loaded with aI-Ad than without. In vitro, dAPCs released from skin explants were found adsorbed preferentially on aI-Ad membranes. Recipient T cells from these mice produced lower concentrations of interferon-gamma cultured ex vivo with donor cells. Taken together, the data indicate that the strategy has the potential to alter the natural course of rejection immune mechanisms in stringent allogeneic models.

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“…30 Administration of low-dose oral CSA (5.0 mg/kg/day) or topical corticosteroid alone modestly prolonged mean survival time from 10 (untreated controls) to 14 and 24 days, respectively. However, coadministration of the two agents significantly prolonged survival, so that four of seven grafts survived beyond 100 days.…”

Section: Topical Therapymentioning

confidence: 92%

Local drug delivery to composite tissue allografts

et al. 2000

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The Synergistic Effect of Low-Dose Cyclosporine and Fluocinolone Acetonide on the Survival of Rat Allogeneic Skin Grafts

Cited by 8 publications

References 0 publications

miR-149-3p Regulates the Switch between Adipogenic and Osteogenic Differentiation of BMSCs by Targeting FTO

miR-149-3p Regulates the Switch between Adipogenic and Osteogenic Differentiation of BMSCs by Targeting FTO

Antibody-functionalized peptidic membranes for neutralization of allogeneic skin antigen-presenting cells

Local drug delivery to composite tissue allografts

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