The synergistic effect of electroacupuncture and bone mesenchymal stem cell transplantation on repairing thin endometrial injury in rats

Xia, Liangjun; Meng, Qingyu; Xi, Jin; Qin, Han; Cheng, Jie; Shen, Jie; Xia, Yun; Shi, Liyun

doi:10.1186/s13287-019-1326-6

Cited by 60 publications

(41 citation statements)

References 66 publications

(64 reference statements)

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“…Generally, the SDF-1/CXCR4 axis is related to stem cell migration [43]. Specifically, CXCR4 and SDF-1 that were released from the injured endometrium regulate the paracrine function and regeneration of BMSCs in the injured endometrium [20]. Therefore, it is possible that the collagen scaffolds with hUCMSCs may reduce fibrosis by activating the Hippo/TAZ signaling pathway and repair the endometrial damage by activating the SDF-1/CXCR4 axis.…”

Section: Discussionmentioning

confidence: 99%

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Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Liu

Cai

Wen

et al. 2020

Gynecol Obstet Invest

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Background: Intrauterine adhesion (IUA) is the second leading cause of secondary infertility in women. Research has shown that stem cells can promote endometrial regeneration and that biomaterials are also helpful in tissue regeneration. Therefore, we compared the efficacy of a collagen scaffold combined with either human umbilical cord mesenchymal stem cells (hUCMSC) or estrogen for the treatment of IUA. Methods: The IUA-induced rats were injected with hUCMSCs or estrogen, and with a collagen scaffold. The endometrial glands and amount of fibrosis were assessed using hematoxylin and eosin and Masson staining. The extent of fibrosis and levels of regeneration-related cytokines were examined by real-time quantitative PCR, and the expression levels of the estrogen receptor, KI67 and cytokeratin were analyzed using an immunochemistry assay. In addition, human nuclear antigen (HuNu) and vimentin were examined by immunofluorescence microscopy. Results: The collagen scaffold administered with hUCMSCs markedly increased the number of endometrial glands and reduced the area of fibrosis compared with either the collagen scaffold or hUCMSCs alone. In addition, the collagen scaffold with hUCMSCs significantly regulated the expression levels of fibrosis, estrogen, and differentiation-related genes relative to the collagen scaffold or hUCMSCs alone. Furthermore, the hUCMSCs alone or in combination with the collagen scaffold increased the expression of HuNu and vimentin in the IUA-induced rat model. In addition, protein levels of the p-transcriptional co-activator with PDZ-binding motif, stromal cell-derived factor-1, and C-X-C chemokine receptor type 4 were upregulated in the group that received the collagen scaffold in combination with hUCMSCs. Conclusion: Our results suggest that the combination of the collagen scaffold with hUCMSCs may be an alternative approach for treating IUA.

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Section: Discussionmentioning

confidence: 99%

“…Total protein lysates were extracted, and the protein concentration in the lysates was quantified using bicinchoninic acid [20]. Protein (50 μg) was loaded into the sodium dodecyl sulfatepolyacrylamide gel, and the separated proteins were then transferred onto polyvinylidene fluoride membranes.…”

Section: Western Blotmentioning

confidence: 99%

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Liu

Cai

Wen

et al. 2020

Gynecol Obstet Invest

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“…Acupuncture can induce a positive feedback effect on the hypothalamic-pituitary-gonad (HPG) axis to improve the function of the reproductive system [51]. Previous animal studies have shown that acupuncture might increase endometrial thickness and endometrial receptivity [39,40].…”

Section: Discussionmentioning

confidence: 99%

“…A signi cant increase in endometrial thickness and the number of endometrial glands after EA treatment, probably due to up-regulation of serum E levels, and different regulation of the sex steroid receptors ERα and ERβ [39]. EA also plays an effective auxiliary role of supporting bone marrow mesenchymal stem cell (BMSCs) in repair of thin endometrium [40]. Acupuncture can affect estrogen level on the day of hCG administration, improve endometrial blood ow state and high-quality embryo rate [41].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Electroacupuncture for Thin Endometrium in Infertile Women: Study Protocol for A Single-Blind, Randomized Controlled Trial

Liu

Wang

et al. 2020

Preprint

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Background: Thin endometrium negatively impacts the reproductive function. Current treatments for thin endometrium do not always improve endometrial receptivity. Preliminary evidence suggests that electroacupuncture could have potential therapy for thin endometrium in infertile women. Thus, this randomized controlled trial was designed to test whether electroacupuncture can improve endometrial receptivity in infertile women with thin endometrium.Methods: This study is a randomized, single-blinded, controlled, clinical trial. A total of 142 eligible patients will be recruited and randomly assigned to the electroacupuncture (EA) group or the sham electroacupuncture (SEA) group in a 1:1 ratio. Participants will receive 36 sessions over three menstrual cycles (12 weeks in total), with the same acupoint prescription. The primary outcome of this trial is endometrial thickness in the midluteal phase. The secondary outcomes include endometrial pattern, resistance index (RI) and pulsatility index (PI) of bilateral uterine artery and endometrium blood flow, serum estradiol (E2) and progesterone (P), and pregnancy rate. The pregnancy rate will be evaluated during a six-month follow-up after completion of the trial. All other outcomes will be evaluated before treatment, during the treatment of 1st, 2nd, and 3rd menstrual cycle, and 6 months after treatment.Discussion: If the outcome confirms the effectiveness of electroacupuncture for thin endometrium in infertile women, this treatment will be proposed for application in clinical practice.Trial registration: Trial registration: Chinese Clinical Trials Registry, ChiCTR2000029983. Registered on 18 February 2020, http://www.chictr.org.cn

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“…Apart from endometrium-derived cells, BMSCs were largely used for endometrium and uterus regeneration due to their convenient isolation, abundant resources and reparative potential (Xia et al, 2019). Ding et al (2014) transplanted BMSCloaded collagen scaffolds to the wounded rat uterine horn.…”

Section: Cell-scaffold Interface-based Endometrium Regenerationmentioning

confidence: 99%

Advances in the Application of Biomimetic Endometrium Interfaces for Uterine Bioengineering in Female Infertility

Han

2020

Front. Bioeng. Biotechnol.

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The Asherman's syndrome, also known as intrauterine adhesion, often follows endometrium injuries resulting from dilation and curettage, hysteroscopic resection, and myomectomy as well as infection. It often leads to scarring formation and female infertility. Pathological changes mainly include gland atrophy, lack of vascular stromal tissues and hypoxia and anemia microenvironment in the adhesion areas. Surgical intervention, hormone therapy and intrauterine device implantation are the present clinical treatments for Asherman's syndrome. However, they do not result in functional endometrium recovery or pregnancy rate improvement. Instead, an increasing number of researches have paid attention to the reconstruction of biomimetic endometrium interfaces with advanced tissue engineering technology in recent decades. From micro-scale cell sheet engineering and cell-seeded biological scaffolds to nanoscale extracellular vesicles and bioactive molecule delivery, biomimetic endometrium interfaces not only recreate physiological multi-layered structures but also restore an appropriate nutritional microenvironment by increasing vascularization and reducing immune responses. This review comprehensively discusses the advances in the application of novel biocompatible functionalized endometrium interface scaffolds for uterine tissue regeneration in female infertility.

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The synergistic effect of electroacupuncture and bone mesenchymal stem cell transplantation on repairing thin endometrial injury in rats

Cited by 60 publications

References 66 publications

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Effectiveness of Electroacupuncture for Thin Endometrium in Infertile Women: Study Protocol for A Single-Blind, Randomized Controlled Trial

Advances in the Application of Biomimetic Endometrium Interfaces for Uterine Bioengineering in Female Infertility

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