The synergistic effect of chitosan-alginate nanoparticle loaded with doxycycline antibiotic against multidrug resist enterobacteriaceae

alsaady, wasan nabeel

doi:10.24996/ijs.2020.61.12.6

Cited by 9 publications

(3 citation statements)

References 40 publications

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“…A possible explanation for this antibacterial activity is the interaction of polycationic CH with negatively charged cell membrane of bacteria, which can lead to intracellular leakage and death of bacteria due to changes in permeability and structure of cell membrane [40]. These results are somewhat consistent with those reported by Kazem and Zidan [41] who found a promising inhibition capacity of a biodegradable and biocompatible nano-formula as an antimicrobial agent.…”

Section: Colony-counting Methodssupporting

confidence: 88%

Improving Oral Intake of Essential Oil Blends Using A Novel Formulation of Biodegradable Chitosan/Lecithin Nanoparticles

Al-Ogaidi

2021

eijs

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A novel, safe and efficient method was developed to encapsulate a blend of essential oils (EOs) into biodegradable nanoparticles (NPs). The biodegradable and biocompatible nanoparticles were made from chitosan (CH) and lecithin (LE) . The quality of the essential oils was verified using gas chromatography/mass spectrometry (GC/MS). The synthesis of nanoparticles included emulsification, followed by sonication, homogenization, and extrusion. Transmission electron microscopy (TEM) indicated that the nanoparticles were spherical in shape with sizes ranging from 25 to 70 nm, while dynamic light scattering (DLS) showed high negative zeta potentials. The stability of the final formula was evaluated in gastric and intestinal fluids. The chitosan/lecithin encapsulated EOs exhibited promising antimicrobial activity against the multi-drug resistant bacteria Salmonella typhi.

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Section: Colony-counting Methodssupporting

confidence: 88%

Improving Oral Intake of Essential Oil Blends Using A Novel Formulation of Biodegradable Chitosan/Lecithin Nanoparticles

Al-Ogaidi

2021

eijs

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“…The results of mixed oppositely charged polysaccharides (chitosan and alginate), observed changes in some bands placement and disappearance or appearance of new peaks in comparison to single alginate or chitosan, and this is associated with possible electrostatic interaction between mixed polymers as mentioned in previous research [41]. A broad band around 3500-3100 cm-1, indicating enhanced hydrogen bonding compared to that of chitosan or sodium alginate alone [42].…”

Section: Fourier Transforms Infrared (Ftir) Characterizationsupporting

confidence: 53%

Evaluation of Chitosan-Alginate Nanoparticle as A Stable Antibacterial Formula in Biological Fluids

Kadhum

Al-Ogaidi

2022

eijs

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This research mainly focuses on the preparation of chitosan-alginate Nanoparticle by ionotropic gelation method using calcium chloride and sodium alginate to form nanocomposites of CH-ALg, examine their antibacterial activity against multidrug resistance (MDR) bacteria, and evaluate the stability of chitosan-alginate formula in different biological fluids, including simulated gastric fluid (SGF) and intestinal fluid (SIF). The average diameter of particles size prepared was measured by an Atomic force microscope (AFM) and it was 61.91 nm. Otherwise, the nature of functional groups present in CH-ALg nanoparticle was investigated by Fourier transforms infrared (FTIR) analysis. The stability of synthesized CH-ALg nanoparticle was measured by Zeta potential measurement and show high stability with a 79 mV ratio. The SEM picture shows that particles are shown to be in the form of bundles and the size of nanoparticles was in the range of (14-84nm). The antibacterial activity of CH-ALg was tested against (MDR) Gram-positive bacteria (staphylococcus aureus) and Gram-negative bacteria (Salmonella typhi, Pseudomonas aeruginosa, and Enterobacter cloacae). The results exhibited a significant impact of antibacterial action against isolates of MDR. CH-ALg nanoparticles were loaded with antibiotic doxycycline in order to strengthen the antibacterial action and drug delivery effectiveness. The synergistic effects of prepared CH-ALg loaded with DOX antibiotic at several concentrations toward MDR bacteria were also examined. The results indicate that DOX-loaded nanoparticles have a significant improvement for antibacterial activity against Gram-negative and Gram-positive compared to doxycycline alone. The formula of chitosan- alginate was stabile in various biological fluids including simulated gastric fluid (SGF) and simulated intestinal fluid (SiF)

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“…AFM was applied to measure the superficial roughness, topography, as well as morphology (AFM). This approach gives atomic level 2D and 3D images of nanoparticles [50]. The 2D and 3D AFM pictures of ZOL-GO were presented in Figure 3, while Figure 4 revealed particle size distribution of ZOL-GO.…”

Section: Atomic Force Microscope Analysesmentioning

confidence: 99%

Anticancer Mechanisms of Zoledronic Acid-Based Graphene Oxide Nanoparticles for Prostate Cancer Bone Metastases Treatment

Rasheed

Al-Sahlanee

Khalfa

2022

Iraqi Journal of Science

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Bone metastases are the main reason for death in males suffering from advanced prostate cancer. This study aimed to create zoledronic acid and graphene oxide conjugation for anticancer therapy. The process of conjugation was confirmed by several characterization methods including UV-VIS spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), and atomic force microscope (AFM). the cytotoxicity of 400, 600, and 800 μg/ml to each GO, ZOL, and ZOL-GO was evaluated on a human hepatic cell line (WRL 68) and human prostate cancer cell line (PC3) using an MTT assay. The antitumor mechanisms of ZOL-GO were examined by cell cycle analysis. The results demonstrated That ZOL-GO caused a reduction in the cell viability of WRL 68 and PC3, with IC50 values of about 932.9 and 787.9 µg/ml respectively. The cell cycle distribution was evaluated after treating the prostate cancer cells (PC3) with 800µg/mL of ZOL-GO, the results showed the presence of a highly significant arresting effect in the G1 phase (P≤ 0.002) than the untreated cells (control). Our findings demonstrate the potential antitumor activity of using GO as a nanocarrier to improve the therapeutic efficacy of prostate cancer.

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The synergistic effect of chitosan-alginate nanoparticle loaded with doxycycline antibiotic against multidrug resist enterobacteriaceae

Cited by 9 publications

References 40 publications

Improving Oral Intake of Essential Oil Blends Using A Novel Formulation of Biodegradable Chitosan/Lecithin Nanoparticles

Improving Oral Intake of Essential Oil Blends Using A Novel Formulation of Biodegradable Chitosan/Lecithin Nanoparticles

Evaluation of Chitosan-Alginate Nanoparticle as A Stable Antibacterial Formula in Biological Fluids

Anticancer Mechanisms of Zoledronic Acid-Based Graphene Oxide Nanoparticles for Prostate Cancer Bone Metastases Treatment

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