The synergistic antitumor effects of all-trans retinoic acid and C-phycocyanin on the lung cancer A549 cells in vitro and in vivo

Li, Bing; Gao, Mei‐Hua; Chu, Xiangcheng; Teng, Lirong; Lv, Cong-Yi; Yang, Peng; Yin, Qi-Feng

doi:10.1016/j.ejphar.2015.01.009

Cited by 77 publications

(38 citation statements)

References 29 publications

(29 reference statements)

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“…The effect observed with betaine correlated to a previous study reporting the decrease in viability following 2% and 4% betaine treatment of Hepa1-6 cells (hepatocellular carcinoma) and HepG2 cells (human hepatoblastoma) [41]. Although there are previous indications that C-PC alone can inhibit cancer cell growth in various concentrations and in a dose-dependent manner by inducing apoptosis [36, 42, 43], we did not observe this. One possible explanation could be the effectiveness of the product, which is highly dependent on the organism of origin, extraction and purification methods, and storage [44, 45].…”

Section: Discussionsupporting

confidence: 82%

Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 CellsIn VitroandIn Vivo

Bingula

Dupuis

Pichon

et al. 2016

Journal of Oncology

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We investigated the effects of betaine, C-phycocyanin (C-PC), and their combined use on the growth of A549 lung cancer both in vitro and in vivo. When cells were coincubated with betaine and C-PC, an up to 60% decrease in viability was observed which is significant compared to betaine (50%) or C-PC treatment alone (no decrease). Combined treatment reduced the stimulation of NF-κB expression by TNF-α and increased the amount of the proapoptotic p38 MAPK. Interestingly, combined treatment induced a cell cycle arrest in G2/M phase for ~60% of cells. In vivo studies were performed in pathogen-free male nude rats injected with A549 cells in their right flank. Their daily food was supplemented with either betaine, C-PC, both, or neither. Compared to the control group, tumour weights and volumes were significantly reduced in either betaine- or C-PC-treated groups and no additional decrease was obtained with the combined treatment. This data indicates that C-PC and betaine alone may efficiently inhibit tumour growth in rats. The synergistic activity of betaine and C-PC on A549 cells growth observed in vitro remains to be further confirmed in vivo. The reason behind the nature of their interaction is yet to be sought.

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Section: Discussionsupporting

confidence: 82%

Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 CellsIn VitroandIn Vivo

Bingula

Dupuis

Pichon

et al. 2016

Journal of Oncology

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“…*p < .05 was significant difference compared with control groups. Also, ATRA combined with C-phycocyanin (C-PC) cooperatively showed an effective anti-tumor activity in human lung cancer cell xenografted animal model in vivo and two-drug combination decreased the expression of CDK-4, but increased caspase-3, leading to cell apoptosis induction (Li et al, 2015). AB means ATRA combined with BDMC metastasis (Lan et al, 2009;Liu et al, 2003) in human cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Combinational treatment of all‐trans retinoic acid (ATRA) and bisdemethoxycurcumin (BDMC)‐induced apoptosis in liver cancer Hep3B cells

et al. 2019

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The effects of two-drug combination, all-trans retinoic acid (ATRA) and bisdemethoxycurcumin (BDMC), on apoptosis induction of liver cancer cells were investigated in human liver Hep 3B cells. Two-drug combination caused a more effective decrease in cell viability and in induction of S phase arrest, DNA damage, and cell apoptosis than that of ATRA or BDMC only. Also, the two-drug combination caused more cells to undergo significantly increased ROS productions when compared to that of ATRA or BDMC only. Results of Western blotting demonstrated that two-drug combination increased expressions of Fas, pro-apoptotic proteins, and active form of caspase-3 and -9, but decreased that of anti-apoptotic proteins and XIAP than that of ATRA or BDMC only in Hep 3B cells. In conclusion, ATRA combined with BDMC enhance cell apoptosis and associated protein expression in Hep 3B cells. Practical applicationsBisdemethoxycurcumin (BDMC) derived from natural plants, turmeric (Curcuma longa), which had been used for Asia food for thousands of years. All-trans retinoid acid (ATRA) is currently used as a primary treatment for patients with acute

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“…It is a natural edible pigment that participates in algal photosynthesis. Over the last two decades, the health benefits of PC have come to be widely accepted 10 , and PC has been reported to have anti-oxidant 11 , anti-inflammatory 12 and anti-tumor 13 activities. Hirahashi et al .…”

Section: Introductionmentioning

confidence: 99%

Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway

et al. 2017

Sci Rep

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Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on days 3, 7 and 28. Data shows PC significantly decreased the levels of hydroxyproline (HYP), vimentin, surfactant-associated protein C (SP-C), fibroblast specific protein-1 (S100A4) and α-smooth muscle actin (α-SMA) but dramatically increased E-cadherin and podoplanin (PDPN) expression on day 28. Moreover, PC greatly decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) at the earlier time. Reduced expression of key genes in the TLR2 pathway was also detected. Compared with WT mice, TLR2-deficient mice exhibited less injury, and the protective effect of PC was partly diminished in this background. These data indicate the anti-fibrotic effects of PC may be mediated by reducing W/D ratio, MPO, IL-6, TNF-α, protecting type I alveolar epithelial cells, inhibiting fibroblast proliferation, attenuating epithelial-mesenchymal transitions (EMT) and reducing oxidative stress. The TLR2-MyD88-NF-κB pathway plays an important role in PC-mediated reduction in pulmonary fibrosis.

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The synergistic antitumor effects of all-trans retinoic acid and C-phycocyanin on the lung cancer A549 cells in vitro and in vivo

Cited by 77 publications

References 29 publications

Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 CellsIn VitroandIn Vivo

Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 CellsIn VitroandIn Vivo

Combinational treatment of all‐trans retinoic acid (ATRA) and bisdemethoxycurcumin (BDMC)‐induced apoptosis in liver cancer Hep3B cells

Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway

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