The Synergic Anti-inflammatory Impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on Intestinal Epithelial Cells in a DSS-induced Colitis Model

Abstract

We investigated the synergic anti-inflammatory activity of Gleditsia sinensis Lam. (GS) extract and Lactobacillus brevis KY21 both in vitro and in vivo. Western blot analysis and immunostaining showed that AKT phosphorylation that increased by the exposure of LPS were significantly decreased by the presence of either GS extract or L. brevis KY21. In addition, p65 intracellular transport was critically inhibited by GS extract and L. brevis KY21. We further studied these effects using an in vivo dextran sulfate sodium (DSS)-induced mouse model. Body weight, food intake, and clinical scores were dramatically decreased after treatment with DSS, whereas these effects were palliated by the addition of GS extract and L. brevis KY21. Importantly, transcription of genes encoding pro-inflammatory cytokines including IL-1β, TNF-α, and IFN-γ in mesenteric lymph nodes (MLN) and the spleen were increased by DSS treatment, whereas they were inhibited by the presence of GS extract and L. brevis KY21.

Effect of the Lactobacillus rhamnosus strain GG and tagatose as a synbiotic combination in a dextran sulfate sodium-induced colitis murine model

“…Cytokines are involved in colitis pathology and several cytokines induced by lactic acid bacteria play key roles in immune regulation (Lee et al, 2011;Kim et al, 2015). We investigated the expression of proinflammatory cytokines IL-6, IL-10, and TNF-α in mice with DSS-induced colitis.…”

Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

“…The colon tissues were processed according to the method described by Kim et al . The deparaffinized colon tissue sections were stained with hematoxylin solution (Sigma‐Aldrich) and eosin Y solution (Daejung, Gyeonggi‐do, Korea).…”

“…The colon tissues were processed according to the method described by Kim et al 25 The deparaffinized colon tissue sections were stained with hematoxylin solution (Sigma-Aldrich) and eosin wileyonlinelibrary.com/jsfa Results are expressed as mean ± SE (n = 12). Means in the same column with different lower-case letters (a, b, c) are significantly different (P < 0.05).…”

Vaccaria segetalis: A Review of Ethnomedicinal, Phytochemical, Pharmacological, and Toxicological Findings

“…Sieb. (Celastraceae), and the seeds of V. segetalis (Neck; Caryophyllaceae), was proven to be effective for treating CPID in the previous studies (Kim et al, 2015 ; Li et al, 2016 ). In this formulation, V. segetalis (Neck) plays an important role because it has analgesic and anti-inflammatory properties, and activates blood circulation by dissipating blood stasis.…”