The syncytial visceral and somatic musculature develops independently of β3-Tubulin during Drosophila embryogenesis, while maternally supplied β1-Tubulin is stable until the early steps of myoblast fusion

Rudolf, Anja; Buttgereit, Detlev; Rexer, Karl‐Heinz; Renkawitz‐Pohl, Renate

doi:10.1016/j.ejcb.2011.11.002

Cited by 9 publications

(9 citation statements)

References 82 publications

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“…βTub97EF expression occurs predominantly in the gut Three of the five β-tubulin paralogs, βTub56D, βTub60D and βTub85D, have been studied in considerable detail (Rudolf et al, 2012 and references therein) but not βTub97EF. Therefore, the pattern of βTub97EF expression during development at 25°C was analyzed.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, anti-pan-β-tubulin E7 revealed maximal levels of total βtubulin at the start of embryogenesis ( Fig. 2A) when the abundant maternally contributed β-Tubulin 56D is present (Rudolf et al, 2012). The monoclonal antibody DM1A (Blose et al, 1984) was used to detect α-tubulins ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The normal expression of βTub60D is also tissue specific, but considerably more complex than that of the testis-specific βTub85D. During embryogenesis, βTub60D expression starts in differentiating mesodermal cell types, but occurs also in chordotonal organs, imaginal discs and somatic cells of the adult gonads (Kimble et al, 1990;Rudolf et al, 2012). The zygotic expression of βTub56D has also pronounced tissue specificity, but at the start of embryogenesis the abundant maternal βTub56D contribution is the only source of β-tubulin (Buttgereit et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

et al. 2017

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Cells in ectotherms function normally within an often wide temperature range. As temperature dependence is not uniform across all the distinct biological processes, acclimation presumably requires complex regulation. The molecular mechanisms that cope with the disruptive effects of temperature variation are still poorly understood. Interestingly, one of five different β-tubulin paralogs, , was among the genes upregulated at low temperature in cultured cells. As microtubules are known to be cold sensitive, we analyzed whether protects microtubules at low temperatures. During development at the optimal temperature (25°C), was expressed in a tissue-specific pattern primarily in the gut. There, as well as in hemocytes, expression was increased at low temperature (14°C). Although mutants were viable and fertile at 25°C, their sensitivity within the well-tolerated range was slightly enhanced during embryogenesis specifically at low temperatures. Changing β-tubulin isoform ratios in hemocytes demonstrated that β-Tubulin 97EF has a pronounced microtubule stabilizing effect. Moreover, is required for normal microtubule stability in the gut. These results suggest that upregulation at low temperature contributes to acclimation by stabilizing microtubules.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

et al. 2017

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“…βTub56D is maternally supplied to the embryo and zygotically expressed during neurogenesis and in muscle attachment sites shortly after the insertion of muscles into the epidermis (7)(8)(9)(10). It was shown that βTub56D is required for myoblast fusion, myotube elongation, and sarcomere formation during Drosophila embryogenesis (11). Recently, it was shown that the βTub97EF function is dispensable for viability and fertility, but it has a tissue-specific requirement for regulation of MT stability in a temperature-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Revisiting the role of beta-tubulin in Drosophila development: beta-tubulin60D is not an essential gene, and its novel Pin¹ allele has a tissue-specific dominant-negative impact

Krishnan

Halachmi²,

Baskar

et al. 2021

Preprint

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Diversity in cytoskeleton organization and function may be achieved through alternative tubulin isotypes and by a variety of post-translational modifications. The Drosophila genome contains five different β-tubulin paralogs, which may play an isotype tissue-specific function in vivo. One of these genes, the beta-tubulin60D gene, which is expressed in a tissue-specific manner, was found to be essential for fly viability and fertility. To further understand the role of the beta-tubulin60D gene, we generated new beta-tubulin60D null alleles (beta-tubulin60D M) using the CRISPR/Cas9 system and found that the homozygous flies were viable and fertile. Moreover, using a combination of genetic complementation tests, rescue experiments, and cell biology analyses, we identified Pin 1, an unknown dominant mutant with bristle developmental defects, as a dominant-negative allele of beta-tubulin60D. We also found a missense mutation in the Pin 1 mutant that results in an amino acid replacement from the highly conserved glutamate at position 75 to lysine (E75K). Analyzing the β-tubulin structure suggests that this E75K alteration destabilizes the alpha-helix structure and may also alter the GTP-Mg2+ complex binding capabilities. Our results revisited the credence that beta-tubulin60D is required for fly viability and revealed for the first time in Drosophila, a novel dominant-negative function of missense beta-tubulin60D mutation in bristle morphogenesis.

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“…We also used rabbit anti-GFP (1:500, Life Technologies. Carlsbad, CA, USA), rabbit anti-Talin [1:500, a gift from Nick Brown (Brown et al, 2002)], rabbit anti-Mef2 [1:1000, gift of Bruce Paterson (Lilly et al, 1995)] and anti-b3-tubulin [1:5000, gift from Renate Renkawitz-Pohl (Rudolf et al, 2012)] antibodies. Samples were imaged using a Nikon Eclipse C1 confocal miscroscope.…”

Section: Luciferase Experiments and Gfp Reportersmentioning

confidence: 99%

Notch directly regulates cell morphogenesis genes, Reck, talin and trio, in adult muscle progenitors

Pézeron

Millen

Boukhatmi

et al. 2014

Journal of Cell Science

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There is growing evidence that Notch pathway activation can result in consequences on cell morphogenesis and behaviour, both during embryonic development and cancer progression. In general, Notch is proposed to co-ordinate these processes by regulating expression of key transcription factors. However, many Notch-regulated genes identified in genome-wide studies are involved in fundamental aspects of cell behaviour, suggesting a more direct influence on cellular properties. By testing the functions of 25 such genes we confirmed that 12 are required in developing adult muscles consistent with roles downstream of Notch. Focusing on three, Reck, rhea/talin and trio, we verify their expression in adult muscle progenitors and identify Notch-regulated enhancers in each. Full activity of these enhancers requires functional binding sites for Su(H), the DNA-binding transcription factor in the Notch pathway, validating their direct regulation. Thus, besides its well-known roles in regulating the expression of cell-fate determining transcription factors, Notch signalling also has the potential to directly affect cell morphology/behaviour by modulating expression of genes such as Reck, rhea/talin and trio. This sheds new light on functional outputs of Notch activation in morphogenetic processes.

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The syncytial visceral and somatic musculature develops independently of β3-Tubulin during Drosophila embryogenesis, while maternally supplied β1-Tubulin is stable until the early steps of myoblast fusion

Cited by 9 publications

References 82 publications

Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

Revisiting the role of beta-tubulin in Drosophila development: beta-tubulin60D is not an essential gene, and its novel Pin¹ allele has a tissue-specific dominant-negative impact

Notch directly regulates cell morphogenesis genes, Reck, talin and trio, in adult muscle progenitors

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The syncytial visceral and somatic musculature develops independently of β3-Tubulin during Drosophila embryogenesis, while maternally supplied β1-Tubulin is stable until the early steps of myoblast fusion

Cited by 9 publications

References 82 publications

Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

Revisiting the role of beta-tubulin in Drosophila development: beta-tubulin60D is not an essential gene, and its novel Pin1 allele has a tissue-specific dominant-negative impact

Notch directly regulates cell morphogenesis genes, Reck, talin and trio, in adult muscle progenitors

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Revisiting the role of beta-tubulin in Drosophila development: beta-tubulin60D is not an essential gene, and its novel Pin¹ allele has a tissue-specific dominant-negative impact