The symptomless leaf infection with grapevine leafroll associated virus 3 in grown in vitro plants as a simple model system for investigation of viral effects on photosynthesis

Christov, I. K.; Stefanov, D.; Velinov, T.; Goltsev, V.; Georgieva, Katya; Abracheva, P.; Genova, Yanka; Christov, N.

doi:10.1016/j.jplph.2005.11.016

Cited by 38 publications

(24 citation statements)

References 44 publications

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“…Induction of sugar transporter genes in photosynthetic organs in response to fungal infection has been correlated with sugar accumulation at the source in Arabidopsis, tomato and grape (Fotopoulos et al 2003;Garcia-Rodriguez and Azcon-Aguilar 2005;Hayes et al 2010). Additionally, it have been described a negative effects in photosynthesis (Christov et al 2007) and accumulation of anthocyanins metabolites (Gutha et al 2010) in leaves from GLRaV-3 infected vines. Together, these results could explain the reduced sugar levels measured in the GLRaV-3 infected fruits under our experimental conditions and the reduction in sugar levels measured in the must of GLRaV-3-infected grapevines (Borgo and Angelini, 2002).…”

Section: Discussionmentioning

confidence: 97%

Compatible GLRaV-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in Vitis vinifera

et al. 2011

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Virus infections in grapevine cause important economic losses and affect fruit quality worldwide. Although the phenotypic symptoms associated to viral infections have been described, the molecular plant response triggered by virus infection is still poorly understood in Vitis vinifera. As a first step to understand the fruit changes and mechanisms involved in the compatible grapevine-virus interaction, we analyzed the berry transcriptome in two stages of development in the red wine cultivar Cabernet Sauvignon infected with Grapevine leaf-roll-associated virus-3 (GLRaV-3). Analysis of global gene expression patterns indicate incomplete berry maturation in infected berries as compared to uninfected fruit suggesting viral infection interrupts the normal berry maturation process. Genes with altered expression in berries harvested from GLRaV-3-infected vines as compared to uninfected tissue include anthocyanin biosynthesis and sugar metabolism genes. The reduction in transcript accumulation for sugar and anthocyanin metabolism during fruit development is consistent with a dramatic reduction in anthocyanin biosynthesis as well as reduced sugar levels in berries, a hallmark phenotypic change observed in virus infected grapevines. Analysis of key regulatory factors provides a mechanism for the observed gene expression changes. Our results provide insight into commonly observed phenotypic alterations in virus infected vines and the molecular mechanisms associated with the plant response to the virus during berry ripening.

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Section: Discussionmentioning

confidence: 97%

Compatible GLRaV-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in Vitis vinifera

et al. 2011

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“…The disturbance of chloroplast functions may be responsible for the production of chlorosis that is associated with virus infection [19]. Several plant-virus interaction studies have revealed that virus infection inhibits host photosynthesis, which is usually accompanied by viral symptoms [7,9,10,16,29]. Virus infection commonly causes chlorosis resulting into impaired chlorophyll biosynthesis and reduced photosynthetic activities that lead to a decrease in biomass production and significant loss in crop yield [44].…”

Section: Introductionmentioning

confidence: 99%

Inducible expression of magnesium protoporphyrin chelatase subunit I (CHLI)-amiRNA provides insights into cucumber mosaic virus Y satellite RNA-induced chlorosis symptoms

et al. 2017

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Recent studies with Y satellite RNA (Y-sat) of cucumber mosaic virus have demonstrated that Y-sat modifies the disease symptoms in specific host plants through the silencing of the magnesium protoporphyrin chelatase I subunit (CHLI), which is directed by the Y-sat derived siRNA. Along with the development of peculiar yellow phenotypes, a drastic decrease in CHLI-transcripts and a higher accumulation of Y-sat derived siRNA were observed. To investigate the molecular mechanisms underlying the Y-sat-induced chlorosis, especially whether or not the reduced expression of CHLI causes the chlorosis simply through the reduced production of chlorophyll or it triggers some other mechanisms leading to the chlorosis, we have established a new experimental system with an inducible silencing mechanism. This system involves the expression of artificial microRNAs targeting of Nicotiana tabacum CHLI gene under the control of chemically inducible promoter. The CHLI mRNA levels and total chlorophyll content decreased significantly in 2 days, enabling us to analyze early events in induced chlorosis and temporary changes therein. This study revealed that the silencing of CHLI did not only result in the decreased chlorophyll content but also lead to the downregulation of chloroplast and photosynthesis-related genes expression and the upregulation of defenserelated genes. Based on these results, we propose that the reduced expression of CHLI could activate unidentified signaling pathways that lead plants to chlorosis.

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“…Van Kooten et al (1990) considered changes in photosynthesis during symptoms development as convenient method for the estimation the altered host metabolism. On the other hand, the presence of symptoms could not always consider as sign for the development of the infection (Barba et al 1989;van Kooten et al 1990;Christov et al 2007). The symptomless infection may also led to reduced photosynthesis (van Kooten et al 1990;Rahoutei et al 2000;Christov et al 2007).…”

Section: Photosynthesis In Inoculated Leaves Of CMV Resistant Pepper mentioning

confidence: 99%

“…On the other hand, the presence of symptoms could not always consider as sign for the development of the infection (Barba et al 1989;van Kooten et al 1990;Christov et al 2007). The symptomless infection may also led to reduced photosynthesis (van Kooten et al 1990;Rahoutei et al 2000;Christov et al 2007). Enhanced alternative electron sinks during the symptomless viral infection were also estimated (Guo et al 2005;Christov et al 2007).…”

Section: Photosynthesis In Inoculated Leaves Of CMV Resistant Pepper mentioning

confidence: 99%

“…When viral-infected plants were grown in vitro at constant environmental conditions they did not show any leaf symptoms but accumulate very high virus concentration in plants ( Barba et al 1989;Christov et al 2001Christov et al , 2007. On the other hand, fluctuating environmental conditions in the field could delay or accelerate the development of disease symptoms causing a great variety in symptom expression in leaves as the disease symptomatic may depend from pathogen strain (van Kooten et al 1990).…”

Section: Introductionmentioning

confidence: 99%

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Accelerated leaf senescence takes part in enhanced resistance in cucumber mosaic virus inoculated pepper leaves

et al. 2011

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Altered photosynthetic reactions in cucumber mosaic virus (CMV) inoculated leaves of virus resistant lines L113 and L57 and susceptible pepper (Capsicum annuum L.) plants cv. Albena grown in controlled environment and in the field were investigated. The CMV inoculated leaves of virus resistant lines developed different symptoms-necrotic local lesions on L113 and chlorotic spots on L57 while the same leaves of susceptible cv. Albena were symptomless. The changes in Photosystem II (PSII) and PSI electron transport were evaluated by chlorophyll fluorescence, and far-red (FR) light induced leaf absorbance A 810-860 . CMV infection caused a decrease in maximal PSII quantum yield, F v /F m , in susceptible leaves. Increased non-photochemical fluorescence quenching in CMV-inoculated leaves of both resistant lines were observed. In CMV-inoculated leaves of all tested plants FR light induced P700 oxidation was decreased. In the present study, the viral-infected pepper plants grown in controlled environment to avoid the effects of abiotic factors were used as model system that allow us to investigate the differences in leaf senescence in CMV-inoculated leaves of susceptible and resistant pepper lines expressing different symptoms. Earlier leaf falls of inoculated leaves as a result of accelerated leaf senescence is important for building successful secondary virus resistance strategy following fast responses such as hypersensitive reaction.

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The symptomless leaf infection with grapevine leafroll associated virus 3 in grown in vitro plants as a simple model system for investigation of viral effects on photosynthesis

Cited by 38 publications

References 44 publications

Compatible GLRaV-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in Vitis vinifera

Compatible GLRaV-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in Vitis vinifera

Inducible expression of magnesium protoporphyrin chelatase subunit I (CHLI)-amiRNA provides insights into cucumber mosaic virus Y satellite RNA-induced chlorosis symptoms

Accelerated leaf senescence takes part in enhanced resistance in cucumber mosaic virus inoculated pepper leaves

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