The susceptibility of pea cultivars to pea seed‐borne mosaic virus infection and virus seed transmission in the UK

Abstract

The reaction of 25 UK pea cultivars to pea seedbornc mosaic virus (PSbMV) was studied in lhe glasshouse. All cultivars proved to be susceptible. When leaf or tendril samples from inoculated plant' wereanalysed, it was found that most combining culuvars had a lower virus content as measured byA4fIj' values in ELISA. than garden and vining cultivars, and that garden and vining cultivars had a simHllr virus content. Seed transmission of PSbMV in 20 cultivars ranged from OOft-t in cvs Maro, Princess an.d" Progreta to 74% in cv. Vedette. There was no obvious relationship between virus content and lhe, efficiency of seed transmission in different cultivars. Wide variation in percentage seed transmission between individuals of single cultivars was observed; for seven cvs (ST 2-49o/Q) tested, all showed some plants escaping seed transmission altogether. Seedlings infected through the seed showed symptonu similar to those in the infected parent plants. Virus particles were readily detectable by immunosorben electron microscopy in infected seedlings, and in elongated infected embryonic shoots produced 3 dayr, after seed germination, by ELISA. An ELISA lest of seed samples from cv. Waverex showed that virutw as as readily detected 3 days after germination as after growth to symptomatic seedlings (4 weeks), an4:p robably provides the most sensitive. accurate and time-saving assay for seed transmission of PSbM,V",

Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus

“…and chickpea (Cicer arietinum L.), as well as Vicia and Lathyrus species (Hampton & Mink, 1975;Makkouk et al, 1993). The seed transmission rate in pea depends on virus isolate, pea cultivar, time of inoculation and environmental conditions (Hampton & Mink, 1975;Khetarpal & Maury, 1987;Kohnen et al, 1995;Wang et al, 1993). We have analysed two isolates of PSbMV, DPD1 and NY, which differ in several biological properties.…”

Indexação do Lettuce mosaic virus em sementes e plântulas de genótipos de alface

“…Wang et al (1993) verificaram o desenvolvimento de plântulas sadias a partir de embriões infectados, o que pode ser devido à baixa concentração ou à localização do vírus nas sementes. A localização do LMV em sementes e plântulas de alface foi observada por Hunter e Bowyer (1993) pela presença de virions e inclusões citoplasmáticas em tecidos embrionários, indicando a replicação viral.…”

“…A infecção viral no tecido apical durante a germinação é um indício de que a taxa de divisão celular excede o movimento do vírus para a plântula. De acordo com Wang et al (1993), a invasão de embriões nem sempre é suficiente para induzir à transmissão do vírus das sementes para as plântulas.…”

Early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus

“…An isolate of PSbMV, PSbMV-28, originally derived from a PSbMV-infected seed of Pisum sativum cv. Waverex (Wang et al, 1992b) was maintained on, and purified (Wang et al, 1992a) Cross-pollination experiments. Healthy plants of cvs.…”

“…Pea cultivars have been identified which vary from a high frequency of seed transmission to zero (Stevenson & Hagedorn, 1969, 1973Wang et al, 1992b) and there is a substantial understanding of the molecular and physiological aspects of pea embryology (Casey, 1990). The complete sequence of PSbMV has been published (Johansen et al, 1991).…”