The Survival Time for Different Bacteria in Various Transport Media

Gästrin, B.; Kallings, Lars Olof; Marcetic, A.

doi:10.1111/j.1699-0463.1968.tb03490.x

Cited by 115 publications

(14 citation statements)

References 10 publications

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“…The ideal transport medium should preserve the number of organisms at the same levels and in the same proportions as at the time of sampling. The modified Stuart transport medium used in this investigation has been thoroughly investgated with regard to these aspects ( Gastrin et al 1968). Staphylococcus aureus was found to be unchanged as regards numbers of viable bacteria over a period of 120 hours.…”

Section: Discussionmentioning

confidence: 99%

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The Microbiological Swab Sampler ‐ a Quantitative Experimental Investigation

Nyström

1978

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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Nystrom. P.-0. The microbiological swab sampler -.4 quantitative experimental investigation. Acta path. microbiol. scand. Sect. B. 86; 361-367, 1978.The aim of the investigation was to give an iri virro experimental account of the commonly used cotton swab as a sampler for quantitative determination of bacteria in operation wounds. The transfer of staphylococci with the swab from an inoculated to a non-inoculated agar culture plate was used as a model, and three different ways of swab handling were tested. A direct transfer of 19 per cent staphylococci from the inoculated to the non-inoculated plate was found. When the swab was rinsed in a small volume of saline and the rinsing fluid was plated, 39 per cent were transferred. When the swab was kept in a Stuart transport medium, as is the usual procedure in clinincal practice. only 4 per cent were transferred. Quantitative wound swab cultures depend on a thorough sampling of the wound and an efficient recovery of bacteria from the swab. I t is concluded that the use of a Stuart transport medium would appear to hamper quantitative determination. and a rinsing procedure should be preferred.

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Section: Discussionmentioning

confidence: 99%

“…The use of charcoal-treated swabs is recommended for neutralizing possible toxic compounds in the medium (Stuart et al. 1954, Ringertz 1960, Gastrin et al 1968). In the present study, no difference was found between ordinary and chard-treated swabs.…”

Section: Discussionmentioning

confidence: 99%

The Microbiological Swab Sampler ‐ a Quantitative Experimental Investigation

Nyström

1978

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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“…The following transport media were used: modified Stuart's (MS) medium (GASTRIN et al, 1968); Amies' modified medium with charcoal (AMIES, 1967) containing 0.4 YO (AM l), 0.6 YO (AM 2) and 1 YO (AM 3) agar, respectively. MS and AM 3 were supplied in rubber-membrane capped tubes by the National Bacteriological Laboratory, S-105 21 Stockholm, Sweden.…”

Section: Transport Mediamentioning

confidence: 99%

“…

The possibility of isolating the contagious equine metritis organism (CEMO) from swabs taken from horses infected with CEMO is enhanced if swabs are sent in transport media of suitable composition (POWELL, 1981; P L A~ and TAYLOR, 1982). Both Stuart's (STUART, 1959) and Amies' (AMIES, 1967) modified transport media are recommended, though the latter is considered superior by some authors (SAHU et al, 1979;TIMONEY et al, 1978).In Sweden, initially all CEM-swabs were transported in modified Stuart's medium (GASTRIN et al, 1968) in cooled containers. When a national control programme was introduced in 1983 (ENGVALL et al, 1983), it was thought desirable to find a transport medium that could be mailed in ordinary envelopes without special cooling arrangements and in which the CEMO would survive for at least 24 hours, which is the most common time taken for mailing a sample to the laboratory.

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confidence: 99%

“…In Sweden, initially all CEM-swabs were transported in modified Stuart's medium (GASTRIN et al, 1968) in cooled containers. When a national control programme was introduced in 1983 (ENGVALL et al, 1983), it was thought desirable to find a transport medium that could be mailed in ordinary envelopes without special cooling arrangements and in which the CEMO would survive for at least 24 hours, which is the most common time taken for mailing a sample to the laboratory.…”

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Survival of Contagious Equine Metritis Organisms (CEMO) in Different Transport Media as Influenced by Storage Time, Temperature and Contaminating Flora

Engvall

1985

Zentralblatt für Veterinärmedizin Reihe B

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Summary A modified Stuart's medium and a modified Amies' medium with three different levels of agar were tested for their suitability as transport media for the contagious equine metritis organism. The modified Stuart's medium was inferior to the other tested media for this purpose. Amies' media with 0.6% agar or 1.0% agar were equally effective as transport media, whereas Amies' medium with 0.4 % agar was liquified when transported. Zusammenfassung Ein modifiziertes Stuart's‐Medium und ein modif. Amies'‐Medium mit drei verschiedenen Agar‐Konzentrationen wurden auf ihre Brauchbarkeit als Transportmedium für den contagious equine metritis‐Erreger untersucht. Das modifizierte Stuart's‐Medium war den anderen getesteten Medien für diesen Zweck unterlegen. Amies'‐Medien mit 0,6% Agar oder 1,0% Agar waren als Transportmedium gleichgut brauchbar, während Amies'‐Medium mit 0,4% Agar beim Transport verflüssigt wurde. Résumé Survivance de l'agent de la métrite équine contagieuse dans différents milieux de transport en fonction du temps de conservation, de la température et de la flore contaminante Un milieu de Stuart modifié et un milieu d'Amies modifié à trois concentrations d'agar différentes ont été étudiés en fonction de leur utilisation comme milieu de transport pour l'agent de la métrite équine contagieuse. Le milieu de Stuart modifié fut inférieur aux autres milieux dans ce but. Les milieux d'Amies avec 0,6% ou 1% d'agar furent également valables pour le transport alors que le milieu d'Amies avec 0,4% d'agar s'est liquifié lors du transport. Resumen La supervivencia de los organismos de la metritis equina contagiosa (CEMO) en vehículos diferentes de transporte a medida que son influenciados por el tiempo de almacenamiento, temperatura y flora contaminate Se analizaron un medio de Stuart modificado y un medio de Amies modificado con tres concentraciones diferentes de agar en cuanto a su aptitud como vehiculo de transporte para el agente etiológico de la metritis equina contagiosa. El medio de Stuart modificado era inferior para esta finalidad a los otros medios contrastados. Los medios de Amies con un 0,6% de agar o 1,0% de agar eran igual de aptos como vehículo de transporte, mientras que el medio de Amies con un 0,4% de agar se licuaba durante el transporte.

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Intestinal Permeability Assessed with Polyethylene Glycols in Children with Diarrhea Due to Rotavirus and Common Bacterial Pathogens in a Developing Community

Johansen,

Stintzing,

Magnusson

et al. 1989

J. pediatr. gastroenterol. nutr.

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Intestinal permeability was assessed with different‐sized polyethylene glycols (PEG 400 and PEG 1,000) in small children with acute diarrhea. All children with acute diarrhea absorbed and excreted less PEG of all molecular sizes into the urine when compared with healthy control children (p < 0.001). Children with acute rotavius infection excreted significantly less PEG of all sizes than children with Shigella, Salmonella, and enteropathogenic Escherichia coli (EPEC) infection (p < 0.001–0.01), suggesting a more severe mucosal lesion caused by rotavirus. In patients with severe malnutrition there was also a significant decrease in absorption of PEGs observed. In addition, malnourished patients with rotavirus diarrhea showed a pronounced decrease of PEGs in comparison with well‐nourished patients. The ratio between the recovery of a large PEG molecule, 1,074 Da, and a small molecule, 370 Da, was utilized to assess the absorption of large molecules in relation to that of smaller ones. On applying this ratio, it was noted that the intestine in children with Shigella and EPEC infection was relatively more permeable to larger molecules than in healthy controls, while in rotavirus and Salmonella infection it was less permeable to larger molecules. In this study significant differences in the permeability characteristics were observed, suggesting etiology‐specific effects on the mucosal barrier.

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The Survival Time for Different Bacteria in Various Transport Media

Cited by 115 publications

References 10 publications

The Microbiological Swab Sampler ‐ a Quantitative Experimental Investigation

The Microbiological Swab Sampler ‐ a Quantitative Experimental Investigation

Survival of Contagious Equine Metritis Organisms (CEMO) in Different Transport Media as Influenced by Storage Time, Temperature and Contaminating Flora

Intestinal Permeability Assessed with Polyethylene Glycols in Children with Diarrhea Due to Rotavirus and Common Bacterial Pathogens in a Developing Community

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