The Survival of Marine Vibrios in Mercenaria Mercenaria, the Hardshell Clam

Greenberg, E. P.; Duboise, Monroe; Palhof, B.

doi:10.1111/j.1745-4565.1982.tb00436.x

Cited by 22 publications

(20 citation statements)

References 18 publications

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“…As already reported in other studies carried out with different shellfish under various experimental conditions (Greenberg et al . 1982; Richards 1988), the present experiments confirm that V. cholerae O1 and V. parahaemolyticus react differently from E. coli to the depuration processes to which molluscs are subjected.…”

Section: Discussionsupporting

confidence: 80%

Effects of depuration of molluscs experimentally contaminated with Escherichia coli, Vibrio cholerae O1 and Vibrio parahaemolyticus

et al. 2002

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Aims: The aim of the present study was to investigate the behaviour of two pathogenic vibrios (Vibrio cholerae O1 and Vibrio parahaemolyticus) during depuration and to compare it with that of Escherichia coli, used as an indicator of suitability for consumption. Methods and Results: Samples of Mytilus galloprovincialis were experimentally contaminated with E. coli, V. cholerae O1 and V. parahaemolyticus, depurated in a pilot plant using ozone and analysed at selected intervals. Numbers of E. coli and vibrios were estimated using an MPN method. The presence of vibrios was con®rmed by the use of PCR. The target genes used were ctx for V. cholerae O1 and the restriction fragment pR72H for V. parahaemolyticus. There was a substantially smaller reduction in the numbers of both vibrios (approximately 1 log) during the depuration process than of E. coli (approximately 3 log). Conclusions:The results con®rm the inadequacy of E. coli as an indicator that molluscs have been cleansed of other microbiological agents. Signi®cance and Impact of the Study: The study con®rms the risk associated with the consumption of mussels and the need to correctly conserve and cook them prior to consumption.

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Section: Discussionsupporting

confidence: 80%

Effects of depuration of molluscs experimentally contaminated with Escherichia coli, Vibrio cholerae O1 and Vibrio parahaemolyticus

et al. 2002

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“…The preference of luminous bacteria towards host gut was experimentally demonstrated by GREENBERG et al (1982) in the clam Mercenaria mercenaria. When the clams were subjected to depuration at temperatures of 8, 15 and 25 "C, V .…”

Section: Tolrrance To Environmental Conditions In the Host Gutmentioning

confidence: 99%

Ecological dynamics of marine luminous bacteria

Alluri¹,

Loganathan²,

Venkateswaran³

1990

J. Basic Microbiol.

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Marine luminous bacteria are heterotrophic, Gram negative microorganisms which continuously emit light. Eight species of luminous bacteria have been described belonging to three different genera, viz. Phorohncreriirni. Vihrio and A~/WOW{JMIS. In this review we report on the distribution of luminous procaryotes in abiotic and biotic components of the marine environment and the interactions among them.Marine luminous bacteria are heterotrophic, gram negative, oxidase positive, facultatively anaerobic, motile rods which emit light continuously. They are ecologically versatile and enjoy more than one life style occurring as free living forms, epiphytes, saprophytes, parasites, symbionts in the light organs of fish and squids and commensals in the gut of various marine organisms.Eight species of luminous bacteria falling under three genera have been reported so h r from the world oceans. They are Plzotobacterium Ieiognntlii, P. plzosphoreum, Vibrio hnrveyi, I/. fisclzeri, V. iogei, V. spendidus biovar. I, V. orientalis and Alteromonus hanedui.Ecology of these microbes is interesting as well as intriguing because. i) They enjoy a variety of life styles such as free living, commensal and symbiotic etc. ii) Some species exhibit restricted and not uniform distribution, for eg. P. Ieiognatlzi is present only in the light organ of leiognathids whereas V. jischeri is symbiotic in the light organ of monocentrids. This type of species specific association is unique.iii) They were reported to exibit seasonal variation in distribution in some environments. iv) Strains of the same species differ in physiological traits depending on the environmental niche from which they are isolated. v) Not many geographical areas have been systematically explored to study their distribution patterns.Given the mounting evidences on their ecological versatility (HASTINGS and NEALSON 1981), role in sea food spoilage (MORII ef al. 1986), detection of toxic substances in the aquatic environment (BULICH 1982, DESILSTRATY 1984, determination of antibiotic activities in dairy and pharmaceutical industries (ULITZUR 1986) and even for catching fish using immobilized luminous bacterial cells (MAKIGUCHI et al. 1980), a comprehensive information on the ecological associations of luminous procaryotes is essential to elucidate the ecological dynamics of these bacteria. With this objective, the distribution of luminous bacteria in abiotic and biotic components of the marine environment and the interactions among them are reviewed here.Bacterial luminescence has been reviewed earlier by NEALSON and HASTINGS (1979), 44 J. Bacic Microbiol 30 (1990) 9

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“…As such, Vibrio spp. appear to selectively colonize, persist, and may even multiply in oysters between the time they are harvested and consumed (8,11,14,16,21,39). Presently, the precise mechanism involved in their persistence is not understood.…”

mentioning

confidence: 99%