The survival of irradiated lactobacilli in the simulated gastrointestinal conditions with antibiotic ceftazidime

Abstract

Significance and Impact of the Study: The probiotic property of lactobacilli is defined with survival in different conditions of human digestive tract even in the presence of antibiotics and subjected to electromagnetic irradiation (EMI) at the extremely high frequency. Despite the fact that EMI and antibiotic ceftazidime affected Lactobacillus acidophilus; the viable number of bacterial cells was decreased in in vitro gastrointestinal model, but they could to grow in fresh growth medium. The changes in the F O F 1 -ATPase activity were obtained at acidic pH. Thus, these bacteria can overcome acid barrier due to the F O F 1 -ATPase: the irradiation stimulates the F O F 1 -ATPase activity in the acidic conditions, but enhances the effects of ceftazidime. The results are important for identifying the mechanisms of lactobacilli survival for physical and chemical factors and valuable for use. AbstractLactobacillus acidophilus is one of the widespread probiotic bacteria that can overcome acid and bile barrier of stomach and intestine, respectively, and then have beneficial effects on the host improving its intestinal microbial balance. The cell membrane F O F 1 -ATPase is an important factor in the response and tolerance to low pH through the action of controlling the H + concentration between the cell cytoplasm and external medium. In this study, the effects of extremely high-frequency EMI at the frequencies of 51Á8 GHz and 53 GHz and cetfazidime ( lmol l À1 ) on survival of L. acidophilus VKM B-1660 in the gastrointestinal model in vitro and on ATPase activity of their membrane vesicles were investigated. Irradiated L. acidophilus survived in media with acid pH; the irradiation stimulated N,N'-dicyclohexylcarbodiimide-sensitive F O F 1 -ATPase activity under acidic conditions, but enhanced the inhibitory effects of ceftazidime. Probably irradiated L. acidophilus is overcoming the acid barrier even in the presence of ceftazidime due to the F O F 1 -ATPase. The obtained results can allow the use of L. acidophilus in food industry, veterinary and medicine.