The survival and recovery of bacteria in vacuum cleaner dust

Haysom, I; Sharp, Kay

doi:10.1177/146642400312300114

Cited by 26 publications

(20 citation statements)

References 14 publications

(18 reference statements)

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“…Vacuums can represent a reservoir for antibiotic resistance genes, and those genes may remain stable and were detectable using a molecular approach in this study, in keeping with previous results for bacteria (8). The possible impact of this reservoir on spreading of drug resistance to humans is unclear.…”

Section: Less (4e6 16s/g Of Dust)supporting

confidence: 75%

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Microbial Contents of Vacuum Cleaner Bag Dust and Emitted Bioaerosols and Their Implications for Human Exposure Indoors

Veillette

Knibbs

Pelletier

et al. 2013

Appl Environ Microbiol

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Vacuum cleaners can release large concentrations of particles, both in their exhaust air and from resuspension of settled dust. However, the size, variability, and microbial diversity of these emissions are unknown, despite evidence to suggest they may contribute to allergic responses and infection transmission indoors. This study aimed to evaluate bioaerosol emission from various vacuum cleaners. We sampled the air in an experimental flow tunnel where vacuum cleaners were run, and their airborne emissions were sampled with closed-face cassettes. Dust samples were also collected from the dust bag. Total bacteria, total archaea, Penicillium/Aspergillus, and total Clostridium cluster 1 were quantified with specific quantitative PCR protocols, and emission rates were calculated. Clostridium botulinum and antibiotic resistance genes were detected in each sample using endpoint PCR. Bacterial diversity was also analyzed using denaturing gradient gel electrophoresis (DGGE), image analysis, and band sequencing. We demonstrated that emission of bacteria and molds (Penicillium/Aspergillus) can reach values as high as 1E5 cell equivalents/min and that those emissions are not related to each other. The bag dust bacterial and mold content was also consistent across the vacuums we assessed, reaching up to 1E7 bacterial or mold cell equivalents/g. Antibiotic resistance genes were detected in several samples. No archaea or C. botulinum was detected in any air samples. Diversity analyses showed that most bacteria are from human sources, in keeping with other recent results. These results highlight the potential capability of vacuum cleaners to disseminate appreciable quantities of molds and human-associated bacteria indoors and their role as a source of exposure to bioaerosols.

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Section: Less (4e6 16s/g Of Dust)supporting

confidence: 75%

“…(5,6) and other bacteria, including Clostridium botulinum (7). Environmental dust could be a source of gastrointestinal infection in the home environment, and the causative microbes collected during vacuum cleaning can remain viable in vacuum dust bags or chambers over extended periods (8).…”

mentioning

confidence: 99%

Microbial Contents of Vacuum Cleaner Bag Dust and Emitted Bioaerosols and Their Implications for Human Exposure Indoors

Veillette

Knibbs

Pelletier

et al. 2013

Appl Environ Microbiol

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“…The only concern raised regarding samples received from the operators was that some boot swabs had dried out by the time they had arrived. Salmonella is able to survive in dry conditions better than other Enterobacteriacae (Haysom and Sharp, 2003), and Salmonella has been recovered from dried litter and faeces up to 26 months old (Davies and Wray, 1996;Davies and Breslin, 2003). Therefore, the drying out of boot swabs may not be of major concern for the detection of high numbers of organisms, but low levels may not survive during transit.…”

Section: Condition Of the Samplesmentioning

confidence: 99%

Investigation of laboratory testing issues in the context of theSalmonellaNational Control Programme in Great Britain

Gosling

Davies

2015

British Poultry Science

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1. The objective of this study was to identify any issues arising during the laboratory testing of samples collected under the National Control Programme for Salmonella. 2. A questionnaire-based survey was conducted among Defra (Department for Environment, Food and Rural Affairs)-approved testing laboratories in order to identify any complicating factors that the laboratories may encounter during the processing of samples. 3. Samples were reported to arrive in good condition and within the specified time frame after collection. The only concern was that new clients may be unaware of the procedure or correct sampling consumables to use. 4. There was evidence of variability between laboratories regarding the sample testing procedure used, with deviation from the guidelines in some cases. 5. This finding suggests that further guidance for laboratories on methodology is likely to be beneficial as this could help improve the detection of low levels of Salmonella.

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“…It has also been reported that aerosolization of carpet dust can be a source to spread other pathogenic bacteria such as Salmonella and Clostridium botulinum [28]. What make things worse is that the presence of such microorganisms in dust bags or the chambers of the vacuum cleaners can be extended over long periods of time [29]. The amount of dust present in the A/C and carpets varied considerably.…”

Section: IVmentioning

confidence: 99%