The surface proteins InlA and InlB are interdependently required for polar basolateral invasion by Listeria monocytogenes in a human model of the blood–cerebrospinal fluid barrier

Gründler, Tim; Quednau, Natascha; Stump, Carolin; Orian-Rousseau, Véronique; Ishikawa, Hiroshi; Wolburg, Hartwig; Schroten, Horst; Tenenbaum, Tobias; Schwerk, Christian

doi:10.1016/j.micinf.2012.12.005

Cited by 59 publications

(62 citation statements)

References 48 publications

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“…In addition, we could show basolateral localization of ABCC1 by means of immunohistochemistry. This fact supports polarity of HIBCPP cells grown in permeable filter inserts, which had been demonstrated before by polarized localization of E-cadherin and the receptor Met (11).…”

Section: Discussionsupporting

confidence: 89%

“…HIBCPP cells were obtained from a CP papilloma of the lateral ventricle from a 29-year-old woman (8) and have been established as a functional model of the BCSFB presenting a high TEER and low permeability for macromolecules (9). Subsequently, HIBCPP cells have been used as an in vitro model of the BCSFB to analyse origin and development of bacterial and viral meningitis (9)(10)(11)(12)(13). Still, the HIBCPP cell line has not yet been characterized with respect to transport protein expression and function, preventing an assessment concerning their suitability for pharmacological research.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier

et al. 2015

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier

et al. 2015

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“…It was suggested that these inconsistent findings may be attributable to differences in experimental conditions, especially involving the addition of normal human serum, which markedly affects InlBmediated invasion due to the presence of anti-Listeria antibodies (231). In an inverted Transwell model of human choroid plexus epithelial cells, the cellular receptors for InlA and InlB (E-cadherin and met receptor tyrosine kinase, respectively) were expressed on the basolateral, "blood" side but not the apical, "CSF" side (232). In this model, L. monocytogenes invaded human choroid plexus epithelial cells exclusively from the basolateral side, and both InlA and InlB were required for efficient invasion.…”

Section: Bacterial Mechanisms Of Brain Invasionmentioning

confidence: 99%

Pathogens Penetrating the Central Nervous System: Infection Pathways and the Cellular and Molecular Mechanisms of Invasion

et al. 2014

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SUMMARY The brain is well protected against microbial invasion by cellular barriers, such as the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). In addition, cells within the central nervous system (CNS) are capable of producing an immune response against invading pathogens. Nonetheless, a range of pathogenic microbes make their way to the CNS, and the resulting infections can cause significant morbidity and mortality. Bacteria, amoebae, fungi, and viruses are capable of CNS invasion, with the latter using axonal transport as a common route of infection. In this review, we compare the mechanisms by which bacterial pathogens reach the CNS and infect the brain. In particular, we focus on recent data regarding mechanisms of bacterial translocation from the nasal mucosa to the brain, which represents a little explored pathway of bacterial invasion but has been proposed as being particularly important in explaining how infection with Burkholderia pseudomallei can result in melioidosis encephalomyelitis.

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“…Although there are many different virulence factors, internalins play a key role for LM in the invasion of non-phagocytic cells [18] . Previous studies have shown that the surface proteins InlA and InlB are interdependently required for the invasion by LM in a human model of the bloodcerebrospinal fluid barrier based on human choroid plexus papilloma cells [13] .…”

Section: Introductionmentioning

confidence: 99%

“…Listeria monocytogenes (LM) is a Gram-positive, facultative intracellular bacterium known to cause invasive disease in humans and animals, especially central nervous system (CNS) infections [13,14] . LM is a food-borne pathogen that typically enters the host through the gastrointestinal tract, from where it can cross the intestinal epithelium.…”

Section: Introductionmentioning

confidence: 99%

Primer Koyun Serebral Mikrodamar Endotelyal ve Astrosit Hücrelerinin Birlikte Kültüre Edilmesiyle Oluşturulmuş In vitro Kan Beyin Bariyeri Modeli

Ren¹,

Yang²,

Yan³

et al. 2018

Kafkas Univ Vet Fak Derg

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In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells. Kafkas Univ Vet Fak Derg, 24 (3): 379-386, 2018379-386, . DOI: 10.9775/kvfd.2017 Abstract Blood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain microvessel endothelial cells and neighbouring elements of the neurovascular unit. It plays a primary role in a selective diffusion barrier at the level of the cerebral microvascular endothelium. Here, we have developed and characterized an in vitro model of the blood-brain barrier by seeding primary brain microvascular endothelial cells (BMECs) and astrocyte cells (ACs) from sheep on a polyester Transwell cell culture membrane with 0.4-μm pores, and conducted transepithelial electrical resistance measurements (TEER), leakage tests and assays for the permeability of horseradish peroxidase (HRP). The results of TEER and 4-h leakage assay showed that the new syngeneic BBB model had a characteristic of BBB in vivo. The HRP permeability test showed that the BBB model had good barrier function. Results showed that our model may be a valuable tool in the study of the mechanisms of brain invasion mediated by internalins of Listeria monocytogenes. Keywords: Blood-brain barrier, Astrocytes, Brain microvascular endothelial cells, Co-culture, In vitro BBB model, Sheep Primer Koyun Serebral Mikrodamar Endotelyal ve Astrosit Hücrelerinin Birlikte Kültüre Edilmesiyle Oluşturulmuş In vitro Kan Beyin Bariyeri Modeli ÖzKan beyin bariyeri beyin mikrodamar endotelyal hücreleri ve nörovasküler birimin komşu elementleri tarafından oluşturulur ve devamlılığı sağlanır. Kan beyin bariyeri serebral mikrovasküler endotel seviyesinde seçici difüzyon bariyeri olarak önemli bir rol oynar. Bu çalışmada 0.4-μm gözeneğe sahip polyester Transwell hücre kültürü membranına koyun primer beyin microdamar endotel hücreleri ve astrosit hücrelerinin ekimiyle in vitro kan beyin bariyeri oluşturuldu. Karakterizasyonu transepitelyal elektrik direnç ölçümleri, sızıntı testleri ve horseradish peroksidaz geçirgenliği testleri ile gerçekleştirildi. Transepitelyal elektrik direnç ölçümleri ve 4-h sızıntı testleri sonuçları yeni oluşturulan singeneik kan beyin bariyeri modelinin in vivo kan beyin bariyeri özelliklerine sahip olduğunu gösterdi. Horseradish peroksidaz geçirgenliği testi yeni oluşturulan kan beyin bariyerinin iyi bariyer fonksiyonlarına sahip olduğunu gösterdi. Elde edilen sonuçlar oluşturulan Listeria monocytogenes internalinleri tarafından oluşturulan beyin invazyonunun mekanizmasının çalışılmasında bu modelin yararlı olabileceğini göstermektedir.

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The surface proteins InlA and InlB are interdependently required for polar basolateral invasion by Listeria monocytogenes in a human model of the blood–cerebrospinal fluid barrier

Cited by 59 publications

References 48 publications

Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier

Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier

Pathogens Penetrating the Central Nervous System: Infection Pathways and the Cellular and Molecular Mechanisms of Invasion

Primer Koyun Serebral Mikrodamar Endotelyal ve Astrosit Hücrelerinin Birlikte Kültüre Edilmesiyle Oluşturulmuş In vitro Kan Beyin Bariyeri Modeli

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