The surface phenotype of swine blood and tissue eosinophil granulocytes

Magyar, Anna; Rudolf, Martin; Oláh, Imré

doi:10.1016/0165-2427(94)05369-4

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…According to Magyar et al. (1994, 1995) and Haverson et al. (1994), subpopulations of myeloid cells (neutrophils, eosinophils, monocytes and macrophages) in swine peripheral blood and lymphoid tissue express SWC3 antigen.…”

Section: Discussionmentioning

confidence: 99%

“…According to Magyar et al (1994Magyar et al ( , 1995 and Haverson et al (1994), subpopulations of myeloid cells (neutrophils, eosinophils, monocytes and macrophages) in swine peripheral blood and lymphoid tissue express SWC3 antigen. The results of the present study on SWC3-expressing cells of the sow endometrium showed that SWC3-expressing cells were commonly found in all layers of the endometrium.…”

Section: Discussionmentioning

confidence: 99%

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The Sow Endometrium at Different Stages of the Oestrous Cycle: Immunohistochemical Study on the Distribution of SWC3‐Expressing Cells (Granulocytes, Monocytes and Macrophages)

Kaeoket

Dalin

Magnusson

et al. 2001

Journal of Veterinary Medicine Series A

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Uterine samples from sows taken immediately after slaughter at late di-oestrus, pro-oestrus, oestrus, early di-oestrus and di-ocstrus, were analysed by immunohistochemistry with an avidinbiotin-peroxidase method using a monoclonal antibody (anti-SWC3) to granulocyte, monocyte and macrophage populations. The endometrium was then examined by light microscopy. In the surface and glandular epithelium, the largest numbers of SWC3-expressing cells (P < or = 0.01 and P < or = 0.05) were found at oestrus, and at pro-oestrus and oestrus, respectively. The numbers of SWC3-expressing cells in the epithelium were positively correlated with the plasma levels of oestradiol-17beta. In the connective tissue of the subepithelial and glandular layers, no significant effect of the oestrous Cycle stage was found on the number of SWC3-expressing cells. The present study showed a variation in the distribution of SWC3-expressing cells in the sow endometrium, especially in the surface and glandular epithelium, during different stages of the oestrous cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Sow Endometrium at Different Stages of the Oestrous Cycle: Immunohistochemical Study on the Distribution of SWC3‐Expressing Cells (Granulocytes, Monocytes and Macrophages)

Kaeoket

Dalin

Magnusson

et al. 2001

Journal of Veterinary Medicine Series A

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“…Flow cytometric analysis of the porcine granulocyte preparations used in the current study revealed the presence of two cell populations that differed in granularity. The side scatter signals of the high SSC and low SSC populations were consistent with those described for EOS (126) and PMN (80), respectively. The identity of the cells could not be absolutely determined by flow cytometry because specific antibodies for identifying porcine EOS were not available at the time of this study.…”

Section: Effect Of Stx2 On the Oxidative Burst Of Porcine Granulocytessupporting

confidence: 83%

Effects of Shiga toxin 2 on isolated porcine granulocytes

Scherer

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Objectives and Specific Aims CHAPTER 2: LITERATURE REVIEW CHAPTER 3: MATERIALS AND METHODS 15 Stx2 and rStxB2 Isolation of Porcine Granulocytes 16 Flow Cytometric Evaluation of Stx2 and rStxB2 Binding and Gb 3 /CD77 Expression by Porcine Granulocytes Stx2 Binding to Porcine Granulocytes at 4°C 17 Flow Cytometric Analysis Fluorescence Microscopic Evaluation of Stx2 Binding Flow Cytometric rStxB2 Binding Assays 20 Flow Cytometric Gb 3 /CD77 Receptor Assay Stx2 and rStxB2 Binding to Porcine Granulocytes Under Conditions Required for Functional Studies 21 Internalization of Stx2 and rStxB2 Effect of Stx2 on Granulocyte Apoptosis Effect of Stx2 on Granulocyte Oxidative Burst Statistical Analysis iii CHAPTER 4: RESULTS Characterization of Granulocyte Preparations 26 Stx2 Binding to Porcine Granulocytes at 4°C 26 Stx2 and rStxB2 Binding and Gb 3 /CD77 Expression at 4°C 27 Stx2 Binding Under Conditions Required for Functional Studies rStxB2 Binding Under Conditions Required for Functional Studies Internalization of Stx2 and rStxB2 Effect of Stx2 on Apoptosis in Porcine Granulocytes Effect of Stx2 on the Oxidative Burst of Porcine Granulocytes Figures CHAPTER 5: DISCUSSION 52

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“…However, this has been documented for human and swine eosinophils that normally express CD4. [29][30][31] The N12 monoclonal antibody and the monoclonal antibody for B cells also bound to eosinophils. Normally, this upper region is separated by gating and ignored when conducting flow cytometric analysis of CD4, N12, or B-cell marker expression on lymphocytes; therefore, binding of a primarily mononuclear cell marker to eosinophils would often be missed.…”

Section: Discussionmentioning

confidence: 97%

Flow cytometric analysis of intracellular complexity and CD45 expression for use in rapid differentiation of leukocytes in bovine blood samples

Pelan-Mattocks

Pesch²,

Kehrli³

2001

ajvr

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This is a rapid assay that is simple to use. More importantly, it is more accurate than the conventional method that involves the use of blood slides and light microscopy, because of the ability of the assay to readily distinguish bovine monocytes and lymphocytes. Rapid preparation of samples and short analysis times allow for efficient and reliable examination of a large number of samples, and the task of viewing slides by light microscopy is eliminated. The labor-savings benefit of this procedure is most apparent in research environments that require frequent processing of batches of blood samples.

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The surface phenotype of swine blood and tissue eosinophil granulocytes

Cited by 15 publications

References 19 publications

The Sow Endometrium at Different Stages of the Oestrous Cycle: Immunohistochemical Study on the Distribution of SWC3‐Expressing Cells (Granulocytes, Monocytes and Macrophages)

The Sow Endometrium at Different Stages of the Oestrous Cycle: Immunohistochemical Study on the Distribution of SWC3‐Expressing Cells (Granulocytes, Monocytes and Macrophages)

Effects of Shiga toxin 2 on isolated porcine granulocytes

Flow cytometric analysis of intracellular complexity and CD45 expression for use in rapid differentiation of leukocytes in bovine blood samples

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