The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels

Hao, Tong; Wen, Ning; Cao, Jun; Wang, H.-B.; Lu, Shuaiyao; Liu, T.; Lin, Qiuxia; Duan, Cuimi; Wang, C.-Y.

doi:10.1016/j.joca.2009.08.007

Cited by 141 publications

(85 citation statements)

References 44 publications

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“…A number of previous studies have investigated the use of chitosan hydrogels for cartilage tissue engineering applications [39][40][41][42][43]. A linear polysaccharide, chitosan consists of β (1→4) linked D-glucosamine residues with a variable number of randomly located N-acetylglucosamine groups and is structurally similar to a number of sGAGs and hyaluronic acids present in articular cartilage [44].…”

Section: Discussionmentioning

confidence: 99%

Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels

et al. 2015

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Cartilaginous tissues engineered using mesenchymal stem cells (MSCs) have been shown to generate bone in vivo by executing an endochondral program. This may hinder the use of MSCs for articular cartilage regeneration, but opens the possibility of using engineered cartilaginous tissues for large bone defect repair. Hydrogels may be an attractive tool in the scaling-up of such tissue engineered grafts for endochondral bone regeneration. In this study, we compared the capacity of different naturally derived hydrogels (alginate, chitosan, and fibrin) to support chondrogenesis and hypertrophy of MSCs in vitro and endochondral ossification in vivo. In vitro, alginate and chitosan constructs accumulated the highest levels of sGAG, with chitosan constructs synthesising the highest levels of collagen. Alginate and fibrin constructs supported the greatest degree of calcium accumulation, though only fibrin constructs calcified homogenously. In vivo, chitosan constructs facilitated neither vascularization nor endochondral ossification, and also retained the greatest amount of sGAG, suggesting it to be a more suitable material for the engineering of articular cartilage.Both alginate and fibrin constructs facilitated vascularization and endochondral bone formation as well as the development of a bone marrow environment. Alginate constructs accumulated significantly more mineral and supported greater bone formation in central regions of the engineered tissue. In conclusion, this study demonstrates the capacity of chitosan hydrogels to promote and better maintain a chondrogenic phenotype in MSCs and highlights the potential of utilizing alginate hydrogels for MSC-based endochondral bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels

et al. 2015

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“…Another possible explanation would be that a longer postoperative time is required for repair tissue remodeling. This is supported by studies carried out in sheep [14] and canine [61] models, where emergence of repair tissue The scaffold fragments are stained and blood vessels can be clearly seen (arrowheads) in the middle of the hollow spaces, which were previously occupied by the degraded scaffold fragments.…”

Section: Discussionmentioning

confidence: 55%

“…It was surprising to find the quality of repair tissue in our control group surpassed the thin layer of sparsely populated fibrous tissue, commonly found to occupy the osteochondral defects of large-animal models [14,25]. This was despite the use of skeletally mature animals, as germinal cells from the physis of skeletally immature animals [1] could contribute to cartilage regeneration.…”

Section: Discussionmentioning

confidence: 83%

“…More than 90% tissue filling was also noted in 2.4-mm [63] and 3.7-mm [41] defects of the rabbit model, which were left untreated for 2 and 3 months, respectively. However, osteochondral defects implanted with hydrogel scaffolds generally have incomplete filling in large animals [14,48]. Differences in repair could be attributed to different intrinsic spontaneous healing capacities between species, as shown by Kon et al who reported the implantation of the same collagen-hydroxyapatite scaffold into sheep [35] and equine [37] models generated inferior fibrocartilaginous tissue in the largersized animal.…”

Section: Discussionmentioning

confidence: 99%

“…This is promising as other osteochondral defect treatments with scaffold only [4,8,14] result in the predominant formation of fibrocartilage, which possesses inferior biomechanical properties [6] and would translate to a shorter repair tissue life span [3].…”

Section: Discussionmentioning

confidence: 99%

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Oligo[poly(ethylene glycol)fumarate] Hydrogel Enhances Osteochondral Repair in Porcine Femoral Condyle Defects

Hui

Ren

Afizah

et al. 2013

Clinical Orthopaedics &Amp; Related Research

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Background Management of osteochondritis dissecans remains a challenge. Use of oligo[poly(ethylene glycol)fumarate] (OPF) hydrogel scaffold alone has been reported in osteochondral defect repair in small animal models. However, preclinical evaluation of usage of this scaffold alone as a treatment strategy is limited.Questions/purposes We therefore (1) determined in vitro pore size and mechanical stiffness of freeze-dried and rehydrated freeze-dried OPF hydrogels, respectively; (2) assessed in vivo gross defect filling percentage and histologic findings in defects implanted with rehydrated freeze-dried hydrogels for 2 and 4 months in a porcine model; (3) analyzed highly magnified histologic sections for different types of cartilage repair tissues, subchondral bone, and scaffold; and (4) assessed neotissue filling percentage, cartilage phenotype, and Wakitani scores. Methods We measured pore size of freeze-dried OPF hydrogel scaffolds and mechanical stiffness of fresh and rehydrated forms. Twenty-four osteochondral defects from 12 eight-month-old micropigs were equally divided into scaffold and control (no scaffold) groups. Gross and histologic examination, one-way ANOVA, and one-way Mann-Whitney U test were performed at 2 and 4 months postoperatively. Results Pore sizes ranged from 20 to 433 lm in diameter. Rehydrated freeze-dried scaffolds had mechanical stiffness of 1 MPa. The scaffold itself increased percentage of

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Chitin – and Chitosan‐Based Scaffolds

2017

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The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels

Abstract: The chitosan-based hydrogel could support matrix accumulation of chondrocytes and could repair sheep cartilage defects in 24 weeks. This study showcased the success of a new technique in its ability to repair articular cartilage defects.

Cited by 141 publications

References 44 publications

Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels

Engineering cartilage or endochondral bone: A comparison of different naturally derived hydrogels

Oligo[poly(ethylene glycol)fumarate] Hydrogel Enhances Osteochondral Repair in Porcine Femoral Condyle Defects

Chitin – and Chitosan‐Based Scaffolds

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