The SUMO conjugation pathway in Populus: genomic analysis, tissue-specific and inducible SUMOylation and in vitro de-SUMOylation

Reed, Jon; Dervinis, Christopher; Morse, Alison M.; Davis, John M.

doi:10.1007/s00425-010-1151-8

Cited by 18 publications

(10 citation statements)

References 46 publications

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“…At the same time, the level of free SUMO was greatly reduced from 1 h of treatment, and when treated for 24 h, most free SUMOs have been exhausted in soybean roots. Consistent with the results got from poplar (Reed et al, 2010 ), SUMOylation by salinity treatment in soybean roots was rapidly inducible and probably has some functional implications.…”

Section: Resultssupporting

confidence: 89%

“…SUMO conjugation was reported to be essential in Arabidopsis , and an increasing number of papers have demonstrated that mutants that were not able to attach SUMO1/2 onto substrate proteins exhibit typical phenotypes such as growth reduction, impaired salinity, drought, heat and freezing tolerance, and altered response to phosphate starvation (Roden et al, 2004 ; Catala et al, 2007 ; Miura et al, 2007b , 2011 ; van den Burg et al, 2010 ). Previous studies with rice ( Oryza sativa ) and poplar ( Populus strichocarpa ) showed that SUMOylation can be significantly enhanced upon heat, cold, high salinity, and abscisic acid exposure (Chaikam and Karlson, 2010 ; Reed et al, 2010 ; Li et al, 2013 ), which was always accompanied by a decrease in the pool of free SUMO and correlated with the duration and intensity of the stress. Interestingly, SUMOylation levels decrease within hours or even minutes when the heat shocked plants returned to the normal temperature, suggesting that SUMOylation acts transiently and reversibly (Kurepa et al, 2003 ; van den Burg et al, 2010 ).…”

Section: Introductionmentioning

confidence: 97%

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Organization and Regulation of Soybean SUMOylation System under Abiotic Stress Conditions

Wang

et al. 2017

Front. Plant Sci.

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Covalent attachment of the small ubiquitin-related modifier, SUMO, to substrate proteins plays a significant role in plants under stress conditions, which can alter target proteins' function, location, and protein-protein interactions. Despite this importance, information about SUMOylation in the major legume crop, soybean, remains obscure. In this study, we performed a bioinformatics analysis of the entire soybean genome and identified 40 genes belonged to six families involved in a cascade of enzymatic reactions in soybean SUMOylation system. The cis-acting elements analysis revealed that promoters of SUMO pathway genes contained different combinations of stress and development-related cis-regulatory elements. RNA-seq data analysis showed that SUMO pathway components exhibited versatile tissue-specific expression patterns, indicating coordinated functioning during plant growth and development. qRT-PCR analysis of 13 SUMO pathway members indicated that majority of the SUMO pathway members were transcriptionally up-regulated by NaCl, heat and ABA stimuli during the 24 h period of treatment. Furthermore, SUMOylation dynamics in soybean roots under abiotic stress treatment were analyzed by western blot, which were characterized by regulation of SUMOylated proteins. Collectively, this study defined the organization of the soybean SUMOylation system and implied an essential function for SUMOylation in soybean abiotic stress responses.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 97%

Organization and Regulation of Soybean SUMOylation System under Abiotic Stress Conditions

Wang

et al. 2017

Front. Plant Sci.

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“…In plants, proteomic and mass spectrometric analyses identified a large number of SUMO substrates implicated in a wide array of biological pathways, implying important roles sumoylation may play in the regulation of diverse plant processes (Elrouby and Coupland, ; Miller and Vierstra, ; Miller et al ., ; Park et al ., ). Indeed, numerous studies in Arabidopsis and other plant species have established biological functions of different components of SUMO conjugation system in various aspects of plant development and stress responses (Park et al ., ; Reed et al ., ). For example, genetic analysis of SUMO conjugation mutants and transgenic (TG) studies in Arabidopsis revealed that sumoylation is essential for viability.…”

Section: Introductionmentioning

confidence: 97%

Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass

Zhou

et al. 2012

Plant Biotechnology Journal

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SummarySumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to environmental stress in plants. To study the feasibility of manipulating SUMO E3 ligase, one of the important components in the sumoylation pathway in transgenic (TG) crop plants for improving overall plant performance under adverse environmental conditions, we have analysed TG creeping bentgrass (Agrostis stolonifera L.) plants constitutively expressing OsSIZ1, a rice SUMO E3 ligase. Overexpression of OsSIZ1 led to increased photosynthesis and overall plant growth. When subjected to water deficiency and heat stress, OsSIZ1 plants exhibited drastically enhanced performance associated with more robust root growth, higher water retention and cell membrane integrity than wild-type (WT) controls. OsSIZ1 plants also displayed significantly better growth than WT controls under phosphatestarvation conditions, which was associated with a higher uptake of phosphate (Pi) and other minerals, such as potassium and zinc. Further analysis revealed that overexpression of OsSIZ1 enhanced stress-induced SUMO conjugation to substrate in TG plants, which was associated with modified expression of stress-related genes. This strongly supports a role sumoylation plays in regulating multiple molecular pathways involved in plant stress response, establishing a direct link between sumoylation and plant response to environmental adversities. Our results demonstrate the great potential of genetic manipulation of sumoylation process in TG crop species for improved resistance to broad abiotic stresses.

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“…Furthermore, heat-induced SUMO conjugates were detected in the nuclear fraction, suggesting that sumoylation is involved in the signaling and early response to heat stress (Saracco et al, 2007). In addition to Arabidopsis and tomato, SUMO gene families are present in other plants such as poplar, grape, sorghum, and rice (Kurepa et al, 2003;Reed et al, 2010;van den Burg et al, 2010). AtSUM1 is expressed from the early embryo stages (globule, heart, and torpedo stage) and its expression is constitutive in most organs (especially in the leaf and root), except for the vasculature, lateral root primordia and the root apex where AtSUM2 is highly expressed.…”

Section: Small Ubiquitin Like Modifier (Sumo)mentioning

confidence: 99%

SUMO and SUMOylation in Plants

Park

Kim

Park

et al. 2011

Molecules and Cells

123

113

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The traditional focus on the central dogma of molecular biology, from gene through RNA to protein, has now been replaced by the recognition of an additional mechanism. The new regulatory mechanism, post-translational modifications to proteins, can actively alter protein function or activity introducing additional levels of functional complexity by altering cellular and sub-cellular location, protein interactions and the outcome of biochemical reaction chains. Modifications by ubiquitin (Ub) and ubiquitin-like modifiers systems are conserved in all eukaryotic organisms. One of them, small ubiquitin-like modifier (SUMO) is present in plants. The SUMO mechanism includes several isoforms of proteins that are involved in reactions of sumoylation and de-sumoylation. Sumoylation affects several important processes in plants. Outstanding among those are responses to environmental stresses. These may be abiotic stresses, such as phosphate deficiency, heat, low temperature, and drought, or biotic stressses, as well including defense reactions to pathogen infection. Also, the regulations of flowering time, cell growth and development, and nitrogen assimilation have recently been added to this list. Identification of SUMO targets is material to characterize the function of sumoylation or desumoylation. Affinity purification and mass spectrometric identification have been done lately in plants. Further SUMO noncovalent binding appears to have function in other model organisms and SUMO interacting proteins in plants will be of interest to plant biologists who dissect the dynamic function of SUMO. This review will discuss results of recent insights into the role of sumoylation in plants.

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The SUMO conjugation pathway in Populus: genomic analysis, tissue-specific and inducible SUMOylation and in vitro de-SUMOylation

Cited by 18 publications

References 46 publications

Organization and Regulation of Soybean SUMOylation System under Abiotic Stress Conditions

Organization and Regulation of Soybean SUMOylation System under Abiotic Stress Conditions

Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass

SUMO and SUMOylation in Plants

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