The Sulfolobus-?Caldariella? group: Taxonomy on the basis of the structure of DNA-dependent RNA polymerases

Zillig, Wolfram; Stetter, Karl O.; Wunderl, Simon; Schulz, Wolfgang A.; Priess, Harro; Scholz, Ingrid

doi:10.1007/bf00446886

Cited by 368 publications

(178 citation statements)

References 17 publications

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“…Strains and growth conditions Cultures of S. solfataricus P2 (DSM 1617, Zillig et al 1980) were grown as reported previously (Brinkman et al 2002). The different carbon sources were added to a final concentration of 0.2% (w/v).…”

Section: Methodsmentioning

confidence: 99%

The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner–Doudoroff pathway

et al. 2007

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Archaea utilize a branched modification of the classical Entner-Doudoroff (ED) pathway for sugar degradation. The semi-phosphorylative branch merges at the level of glyceraldehyde 3-phosphate (GAP) with the lower common shunt of the Emden-Meyerhof-Parnas pathway. In Sulfolobus solfataricus two different GAP converting enzymes-classical phosphorylating GAP dehydrogenase (GAPDH) and the non-phosphorylating GAPDH (GAPN)-were identified. In Sulfolobales the GAPN encoding gene is found adjacent to the ED gene cluster suggesting a function in the regulation of the semi-phosphorylative ED branch. The biochemical characterization of the recombinant GAPN of S. solfataricus revealed that-like the well-characterized GAPN from Thermoproteus tenax-the enzyme of S. solfataricus exhibits allosteric properties. However, both enzymes show some unexpected differences in co-substrate specificity as well as regulatory fine-tuning, which seem to reflect an adaptation to the different lifestyles of both organisms. Phylogenetic analyses and database searches in Archaea indicated a preferred distribution of GAPN (and/or GAP oxidoreductase) in hyperthermophilic Archaea supporting the previously suggested role of GAPN in metabolic thermoadaptation. This work suggests an important role of GAPN in the regulation of carbon degradation via modifications of the EMP and the branched ED pathway in hyperthermophilic Archaea.Keywords Glyceraldehyde-3-phosphate Á Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase Á GAPN Á Aldehyde dehydrogenase superfamily Á Branched Entner-Doudoroff pathway Á Carbohydrate metabolism Á Archaea Abbreviations EDEntner-Doudoroff sp Semi-phosphorylative np Non-phosphorylative EMP Embden-Meyerhof-Parnas G1P

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Section: Methodsmentioning

confidence: 99%

The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner–Doudoroff pathway

et al. 2007

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“…Mainly on the basis of its similar morphology and its serologically crossreacting RNA Polymerase, Brierley's isolate was described as Sulfolobus brierleyi (Zillig et al, 1980). Further investigations demonstrated that S. brierleyi was a facultative anaerobe.…”

Section: Organisms Able To Extract Metal Ions From Sulfidic Ores ("Bimentioning

confidence: 99%

Metallosphaera sedula gen, and sp. nov. Represents a New Genus of Aerobic, Metal-Mobilizing, Thermoacidophilic Archaebacteria

Huber

Spinnler

Gambacorta

et al. 1989

Systematic and Applied Microbiology

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247

182

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“…In addition, the Sulfolobaceae possess glycoprotein subunit cell envelopes and caldariellaquinone (Weiss, 1974;Zillig et al, 1980;Huber et al, 1989;de Rosa and Gambacorta, 1988). The different genera within the Sulfolobaceae can be distinguished from each other by their metabolic and biochemical properties: Members of Sulfolobus exhibit a GC-content of around 37 mol% and are able to utilize sugars, amino acids, and complex organic substances as energy and carbon sources (Brock et al, 1972;Zillig et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

Sulfolobus metallicus, sp. nov., a Novel Strictly Chemolithoautotrophic Thermophilic Archaeal Species of Metal-Mobilizers

Huber

Stetter

1991

Systematic and Applied Microbiology

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134

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SummaryFive new isolates of archaeal coccoid thermoacidophiles were obtained from Icelandic solfataric fields. They are strict chemolithoautotrophs gaining energy by oxidation of S° and sulfidic ores. The new strains grow between 50 and 75 °C and pH 1 and 4.5 and tolerate NaCl concentrations of up to 3.0%. The GCcontent of their DNA is 38 mol%.The new isolates resemble members of Sulfolobus in their morphology, their ability to oxidize reduced sulfur compounds and their GC-content. They are different in their strictly chemolithoautotrophic mode of life, their ore-leaching capacity, DNA/DNA hybridization and incomplete serological cross-reaction of RNA polymerase. Therefore, we describe here a new species, Sulfolobus metallicus. Type strain is Sulfolobus metallicus (Kra 23; DSM 6482).

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The Sulfolobus-?Caldariella? group: Taxonomy on the basis of the structure of DNA-dependent RNA polymerases

Cited by 368 publications

References 17 publications

The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner–Doudoroff pathway

The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner–Doudoroff pathway

Metallosphaera sedula gen, and sp. nov. Represents a New Genus of Aerobic, Metal-Mobilizing, Thermoacidophilic Archaebacteria

Sulfolobus metallicus, sp. nov., a Novel Strictly Chemolithoautotrophic Thermophilic Archaeal Species of Metal-Mobilizers

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