The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: which isomer for what purpose?

Schiller, Jürgen; Süß, Rosmarie; Fuchs, Beate; Müller, Mat­thias; Petković, Marijana; Zschörnig, Olaf; Waschipky, Hanka

doi:10.1007/s00249-006-0090-6

Cited by 126 publications

(120 citation statements)

References 32 publications

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“…Like DHB [27], where small isomeric changes have a dramatic effect on the efficacy of its behavior as a MALDI matrix, a similar effect is observed here. Clearly, not every rhodamine dye behaves the same as a matrix.…”

Section: Discussionsupporting

confidence: 64%

A useful binary matrix for visible-MALDI of low molecular weight analytes

Yang

Лобода³

et al. 2010

J. Am. Soc. Mass Spectrom.

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In this work, a new absorbing candidate, rhodamine (R) 575, is described, which forms the basis of a binary matrix operating at 532 nm. Analyte ionization is found to be much more efficient when the dye is combined with a proton donor such as hydrochloric acid or ␣-cyano-4-hydroxycinnamic acid, or a proton acceptor such as sodium hydroxide. This makes the matrix more generic than many others that have been tried. Furthermore, under visible illumination R575 produces very few chemical fragments, making it useful for small molecular weight analyte detection. Spectra for a variety of analytes are shown. Insight into the MALDI mechanism was obtained by comparing the similarities and differences of visible-MALDI with the more common UV and IR-MALDI strategies. (J Am Soc Mass Spectrom 2010, 21, 294 -299) © 2010 American Society for Mass Spectrometry O ver the last two decades, matrix assisted laser/ desorption ionization (MALDI) mass spectrometry (MS), using either ultraviolet (UV) [1,2] or infrared (IR) lasers [3], has become an important analytical tool for the analysis of molecules of chemical and biological interest. The success of MALDI-MS is largely due to the relative "softness" of this technique, which allows for ionization and detection of intact analytes with minimal fragmentation. IR-MALDI has been shown to be superior to UV-MALDI in this regard for the analysis of biological macromolecules including DNA and RNA [4], although the former technique typically operates at higher pulse energies and features higher sample consumption per laser pulse. Today, UV lasers and, in particular, the N 2 gas lasers with an output wavelength of 337 nm and Nd:YAG lasers with a wavelength of 355 nm dominate in MALDI-MS instruments; and they are usually less expensive than their IR counterparts.One could expect that MALDI mass spectrometry using visible wavelength lasers will have several advantages over UV-MALDI and IR-MALDI. Many macromolecules of interest absorb UV-light but are transparent at visible wavelengths. Evidence suggests that visible-MALDI is softer than UV-MALDI [5]. Similar to UV-MALDI, visible-MALDI is expected to require lower pulse energies than IR-MALDI. Furthermore, readily available pulsed visible laser sources such as the solid-state frequency-doubled Nd:YAG laser (532 nm wavelength) are becoming relatively inexpensive and have longer operational lifetimes compared with standard UV devices. Delivery of light via fiber optics is also more routine at visible wavelengths.Despite its potential, visible MALDI has not been extensively explored. Studies have dealt with rhodamine (R) B, R6G [5,6], neutral red [7], and 2-amino-3-nitrophenol [8] as possible matrices for visible-MALDI using the 532 nm output of a doubled Nd:YAG laser. Recently, Au-assisted visible laser MALDI has emerged [9] as a new analysis technique as well as visible surface-assisted laser desorption/ionization from a graphite substrate [10]. Our group recently studied visible-MALDI coumarin laser dyes as potential MALDI matrixes using the 480 ...

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Section: Discussionsupporting

confidence: 64%

A useful binary matrix for visible-MALDI of low molecular weight analytes

Yang

Лобода³

et al. 2010

J. Am. Soc. Mass Spectrom.

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“…5B and C. These DHB matrix clusters were similar to those reported in a previous study. 50 Fig. 5C shows that the lipid profile was poor when the matrix concentration was <2 mM.…”

Section: Effects Of Experimental Conditions On Lipid Profilingmentioning

confidence: 99%

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

et al. 2012

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Rapid identification of Bacillus spores has attracted great interest in the medical and forensic communities due to the highly pathogenic nature of certain Bacillus species and its potential application in bioweapons. In this study, we evaluated the feasibility of rapid and reliable identification of several Bacillus spp. (B. subtilis, B. cereus, B. megaterium, B. pumilus, and B. sphaericus) using online MALDI-TOF mass spectrometry combined with lipid profiling. The bacterial cultures in liquid medium were extracted with methanol and chloroform, and the extracts were aerosolized into droplets and immediately analyzed with an online MALDI-TOF mass spectrometer. Results showed that phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and diglycosyldiacylglycerol (DGDG) were present in the mass spectra, as alkali metal adducts. The unique lipid profiles obtained from these Bacillus spp. allowed us to differentiate between them. The reliability and reproducibility of the rapid identification method for Bacillus were tested under different experimental conditions. An alternative online chemotaxonomic method for bacterial identification is demonstrated.

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“…Various matrices have been used for this purpose, but the most established one is 2,5-dihydroxybenzoic acid (DHB) [1]. This matrix does not interfere with peaks arising from analytes, does not lead to marked fragmentation reactions and can be used for the acquisition of positive as well as of negative ion mass spectra [5]. Additionally, in contrast to matrices with olefinic residues, DHB has only a weak tendency to give signals of matrix oligomers [1,5].…”

Section: Introductionmentioning

confidence: 99%

Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids

Petković

Schiller

Müller

et al. 2009

Zeitschrift Für Naturforschung B

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It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-offlight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix -2,5-dihydroxybenzoic acid -yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.

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The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: which isomer for what purpose?

Cited by 126 publications

References 32 publications

A useful binary matrix for visible-MALDI of low molecular weight analytes

A useful binary matrix for visible-MALDI of low molecular weight analytes

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids

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