The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance

Rosales-Reyes, Roberto; Saldías, M. Soledad; Aubert, Daniel; El-Halfawy, Omar M.; Valvano, Miguel A.

doi:10.1099/mic.0.060988-0

Cited by 20 publications

(17 citation statements)

References 54 publications

(60 reference statements)

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“…Heteroresistance to PmB was confirmed by E-test, which demonstrated small colonies growing within the zone of inhibition surrounding the highest concentrations of PmB on the E-test strips, both in K56-2 and K56-2Δ rpoE (Figure 1B and C respectively). A similar pattern of heteroresistance was also previously observed for the K56-2Δ suhB [8], which lacks an inositol monophosphatase and like K56-2Δ rpoE , has intermediate sensitivity to PmB. Heteroresistance to PmB was also observed in the B. cenocepacia clinical isolate CP706-J, indicating that it is not a phenomenon unique to a single strain (Figure1D).…”

Section: Resultssupporting

confidence: 81%

Chemical Communication of Antibiotic Resistance by a Highly Resistant Subpopulation of Bacterial Cells

2013

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The overall antibiotic resistance of a bacterial population results from the combination of a wide range of susceptibilities displayed by subsets of bacterial cells. Bacterial heteroresistance to antibiotics has been documented for several opportunistic Gram-negative bacteria, but the mechanism of heteroresistance is unclear. We use Burkholderia cenocepacia as a model opportunistic bacterium to investigate the implications of heterogeneity in the response to the antimicrobial peptide polymyxin B (PmB) and also other bactericidal antibiotics. Here, we report that B. cenocepacia is heteroresistant to PmB. Population analysis profiling also identified B. cenocepacia subpopulations arising from a seemingly homogenous culture that are resistant to higher levels of polymyxin B than the rest of the cells in the culture, and can protect the more sensitive cells from killing, as well as sensitive bacteria from other species, such as Pseudomonas aeruginosa and Escherichia coli. Communication of resistance depended on upregulation of putrescine synthesis and YceI, a widely conserved low-molecular weight secreted protein. Deletion of genes for the synthesis of putrescine and YceI abrogate protection, while pharmacologic inhibition of putrescine synthesis reduced resistance to polymyxin B. Polyamines and YceI were also required for heteroresistance of B. cenocepacia to various bactericidal antibiotics. We propose that putrescine and YceI resemble "danger" infochemicals whose increased production by a bacterial subpopulation, becoming more resistant to bactericidal antibiotics, communicates higher level of resistance to more sensitive members of the population of the same or different species.

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Section: Resultssupporting

confidence: 81%

Chemical Communication of Antibiotic Resistance by a Highly Resistant Subpopulation of Bacterial Cells

2013

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“…An example of this is suhB , a global virulence regulator controlling the type III and type VI secretion systems, flagellum biosynthesis, and biofilm formation in the human pathogens Burkholderia cenocepacia and Pseudomonas aeruginosa (Rosales-Reyes et al, 2012; Li et al, 2013). Similarly, a P. aeruginosa orthologue of the in planta induced type IV secretion gene Rhs has been described as a virulence determinant (Kung et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…Although classified as a phosphate mobilization gene (Table 3), suhB is a super-regulator involved in the proper rRNA folding (Singh et al, 2016). It plays a role in virulence of animal bacterial pathogens, influencing T3SS, T6SS, flagellum and biofilm regulation and probably acts in opposite ways in different bacteria (Rosales-Reyes et al, 2012; Li et al, 2013). Interestingly, SuhB differential expression was also observed in two R. solanacearum strains (Meng et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomes of Ralstonia solanacearum during Root Colonization of Solanum commersonii

et al. 2017

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Bacterial wilt of potatoes—also called brown rot—is a devastating disease caused by the vascular pathogen Ralstonia solanacearum that leads to significant yield loss. As in other plant-pathogen interactions, the first contacts established between the bacterium and the plant largely condition the disease outcome. Here, we studied the transcriptome of R. solanacearum UY031 early after infection in two accessions of the wild potato Solanum commersonii showing contrasting resistance to bacterial wilt. Total RNAs obtained from asymptomatic infected roots were deep sequenced and for 4,609 out of the 4,778 annotated genes in strain UY031 were recovered. Only 2 genes were differentially-expressed between the resistant and the susceptible plant accessions, suggesting that the bacterial component plays a minor role in the establishment of disease. On the contrary, 422 genes were differentially expressed (DE) in planta compared to growth on a synthetic rich medium. Only 73 of these genes had been previously identified as DE in a transcriptome of R. solanacearum extracted from infected tomato xylem vessels. Virulence determinants such as the Type Three Secretion System (T3SS) and its effector proteins, motility structures, and reactive oxygen species (ROS) detoxifying enzymes were induced during infection of S. commersonii. On the contrary, metabolic activities were mostly repressed during early root colonization, with the notable exception of nitrogen metabolism, sulfate reduction and phosphate uptake. Several of the R. solanacearum genes identified as significantly up-regulated during infection had not been previously described as virulence factors. This is the first report describing the R. solanacearum transcriptome directly obtained from infected tissue and also the first to analyze bacterial gene expression in the roots, where plant infection takes place. We also demonstrate that the bacterial transcriptome in planta can be studied when pathogen numbers are low by sequencing transcripts from infected tissue avoiding prokaryotic RNA enrichment.

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“…The biofilm production was quantified as previously described [17]. Briefly, 5 ml of overnight cultures grown at 37°C were diluted to OD 600 0.003 in LB media, and triplicate 500-μl aliquots were dispensed into polystyrene tubes.…”

Section: Methodsmentioning

confidence: 99%

Biofilm Formation and Susceptibility to Polymyxin B by a Highly Prevalent Clone of Multidrug-Resistant <b><i>Acinetobacter baumannii</i></b> from a Mexican Tertiary Care Hospital

Rosales-Reyes¹,

Alcántar‐Curiel²,

Jarillo-Quijada³

et al. 2015

Chemotherapy

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Background:Acinetobacter baumannii has emerged as a major cause of hospital-associated infections with increased morbidity and mortality among those affected. Methods: A total of 85 isolates of a highly prevalent multidrug-resistant clone, identified during the period 2007-2011, were analyzed for biofilm formation on a polystyrene surface. The minimal inhibitory concentration was determined by the Sensititre System, the agar disk diffusion method and then read by means of the BIOMIC system and serial dilutions on Müller-Hinton agar. Results: In this study, covering a period of 5 years (2007-2011), we demonstrate that a particular clone emerged as the most prevalent, with an associated lethality of 28.2%. We demonstrate that 92.9% of strains corresponding to this clone are biofilm producers. Our results also demonstrate that all isolates were 100% susceptible to polymyxin B. Conclusion: Our study suggests that the high prevalence and lethality of this multidrug-resistant clone of A. baumannii and its persistence over close to 5 years in a Mexican tertiary hospital environment can be explained in part by the ability of these clinical isolates of A. baumannii to form biofilms.

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The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance

Cited by 20 publications

References 54 publications

Chemical Communication of Antibiotic Resistance by a Highly Resistant Subpopulation of Bacterial Cells

Chemical Communication of Antibiotic Resistance by a Highly Resistant Subpopulation of Bacterial Cells

Transcriptomes of Ralstonia solanacearum during Root Colonization of Solanum commersonii

Biofilm Formation and Susceptibility to Polymyxin B by a Highly Prevalent Clone of Multidrug-Resistant <b><i>Acinetobacter baumannii</i></b> from a Mexican Tertiary Care Hospital

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