The subthalamic corticotropin-releasing hormone neurons mediate adaptive REM-sleep responses to threat

Tseng, Yu-Ting; Zhao, Binghao; Chen, Shan-Ping; Ye, Jialin; Liu, Jingjing; Liang, Lisha; Ding, Hui; Schaefke, Bernhard; Yue, Qin; Wang, Lina; Feng, Wenfang

doi:10.1016/j.neuron.2021.12.033

Cited by 43 publications

(19 citation statements)

References 98 publications

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“…Interestingly, we identified a population of RMTg GABAergic neurons that quickly and selectively arouse mice at REM sleep by disinhibiting glutamatergic LDT neurons. A recent study revealed a similar finding, in which activation of the subthalamic corticotropin-releasing hormone neurons mediates waking from REM sleep in response to predator odors 74 . These findings further our understanding of how the brain controls arousal at different sleep stages when animals are challenged with dangerous situations or biologically relevant stimuli.…”

Section: Discussionmentioning

confidence: 58%

GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression

et al. 2022

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Rapid eye movement (REM) sleep disturbances are prevalent in various psychiatric disorders. However, the neural circuits that regulate REM sleep remain poorly understood. Here, we found that in male mice, optogenetic activation of rostromedial tegmental nucleus (RMTg) GABAergic neurons immediately converted REM sleep to arousal and then initiated non-REM (NREM) sleep. Conversely, laser-mediated inactivation completely converted NREM to REM sleep and prolonged REM sleep duration. The activity of RMTg GABAergic neurons increased to a high discharge level at the termination of REM sleep. RMTg GABAergic neurons directly converted REM sleep to wakefulness and NREM sleep via inhibitory projections to the laterodorsal tegmentum (LDT) and lateral hypothalamus (LH), respectively. Furthermore, LDT glutamatergic neurons were responsible for the REM sleep-wake transitions following photostimulation of the RMTgGABA-LDT circuit. Thus, RMTg GABAergic neurons are essential for suppressing the induction and maintenance of REM sleep.

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Section: Discussionmentioning

confidence: 58%

GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression

et al. 2022

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“…PSTN Crh neurons are activated by refeeding (14) and Crh expression in the PSTN is upregulated by an anorexia-inducing valine-deficient diet (9), but they do not influence food consumption (14 and results reported here). Aside from consummatory behaviors, PSTN Crh neurons have been shown to mediate defensive responses to acute predator threats and to regulate REM sleep (34).…”

Section: Discussionmentioning

confidence: 99%

The parasubthalamic nucleus refeeding ensemble delays feeding initiation

Dunning

Lopez

Krull

et al. 2023

Preprint

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The parasubthalamic nucleus (PSTN) is responsive to refeeding after food deprivation and PSTN subpopulations can suppress feeding. However, no study directly addressed the role of PSTN neurons activated upon food access resumption. Here we show that the ensemble of refeeding-activated PSTN neurons massively increases the latency to initiate refeeding with both familiar and novel food but exerts limited control over the amount of food consumed by hungry mice. This ensemble also delays sucrose consumption but accelerates water consumption in thirsty mice. We next sought to identify which subpopulations of PSTN neurons might be driving these effects. We discovered that PSTN Tac1 neurons projecting to the CeA selectively suppress feeding initiation while PSTN Crh neurons surprisingly promote the consumption of novel, palatable substances. Our results demonstrate the key role of endogenous PSTN activity in the control of feeding initiation and identify PSTN subpopulations counteracting each others influence on consummatory behaviors.

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“…Performance and capabilities of three configurations of the MiniLFOV, i.e., 1.8-mm-WD, 3.5-mm-WD, and wire-free, are listed in Table 1 . Furthermore, with independently controlled dual-LED excitation, the MiniLFOV can support future two-color excitation configurations by changing one of the LEDs as well as changing filters and dichroic mirror 49 . With a mass of only 13.9 grams, it is feasible for two MiniLFOVs to be mounted onto the skull of a rat for multi-region imaging.…”

Section: Discussionmentioning

confidence: 99%

Miniscope-LFOV: A large field of view, single cell resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals

Guo

Blair

Sehgal

et al. 2021

Preprint

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We present a large field of view (FOV) open-source miniature microscope (MiniLFOV) designed to extend the capabilities of the UCLA Miniscope platform to large-scale, single cell resolution neural imaging in freely behaving large rodents and head-fixed mice. This system is capable of multiple imaging configurations, including deep brain imaging using implanted optical probes and cortical imaging through cranial windows. The MiniLFOV interfaces with existing open-source UCLA Miniscope DAQ hardware and software, can achieve single cell resolution imaging across a 3.6 × 2.7 mm field of view at 23 frames per second, has an electrically adjustable working distance of up to 3.5 mm±150 µm using an onboard electrowetting lens, incorporates an absolute head-orientation sensor, and weighs under 14 grams. The MiniLFOV provides a 30-fold larger FOV and yields 20-fold better sensitivity than Miniscope V3, and a 12-fold larger FOV with 2-fold better sensitivity than Miniscope V4. Power and data transmission are handled through a single, flexible coaxial cable down to 0.3 mm in diameter facilitating naturalistic behavior. We validated the MiniLFOV in freely behaving rats by simultaneously imaging >1000 GCaMP7s expressing neurons in the CA1 layer of the hippocampus and in head-fixed mice by simultaneously imaging ∼2000 neurons in the mouse dorsal cortex through a 4 × 4 mm cranial window. For freely behaving experiments, the MiniLFOV supports optional wire-free operation using a 3.5 g wire-free data acquisition expansion board which enables close to 1-hour of wire-free recording with a 400 mAh (7.5 g) on-board single-cell lithium-polymer battery and expands wire-free imaging techniques to larger animal models. We expect this new open-source implementation of the UCLA Miniscope platform will enable researchers to address novel hypotheses concerning brain function in freely behaving animals.

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The subthalamic corticotropin-releasing hormone neurons mediate adaptive REM-sleep responses to threat

Cited by 43 publications

References 98 publications

GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression

GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression

The parasubthalamic nucleus refeeding ensemble delays feeding initiation

Miniscope-LFOV: A large field of view, single cell resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals

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