The substrate selection and spawning behaviour of pikeperch Sander lucioperca L. broodstock under pond conditions

Abstract

Spawning substrate is a critical factor in reproduction of commercially valuable percids. Substrate preferences of pikeperch Sander lucioperca were investigated using three types of artificial spawning substrates: long fibred brush, artificial turf and smooth plastic. There was a significantly higher preference for the brush nest, with thick rigid structures, than for artificial turf, while plastic nests remained unoccupied.The majority of pikeperch pairs (94.4%) spawned within 14 days of the beginning of the experiment. No difference was observed between substrates in time from stocking of fish to nest occupation and spawning. After egg laying, nests were moved to controlled conditions of a recirculation aquaculture system for incubation. Hatching rate and larva production did not differ significantly among tested substrates. Either used substrate was suitable for spawning and incubation in a recirculation aquaculture system. Obtained results can be used for semi-artificial reproduction as well as for the support and control of wild pikeperch stocks in natural habitats. K E Y W O R D S artificial nest, broodstock, egg incubation, reproduction, Sander lucioperca ). Information about substrate selectivity and the minimum quantity of suitable spawning grounds How to cite this article: Malinovskyi O, Veselý L, Blecha M, Křišťan J, Policar T. The substrate selection and spawning behaviour of pikeperch Sander lucioperca L. broodstock under pond conditions. Aquac Res. 2018;49:3541-3547.

Improvements on Live Feed Enrichments for Pikeperch (Sander lucioperca) Larval Culture

“…The trial was run at the University of South Bohemia, Facility of Fisheries and Protection of Waters, Czech Republic (USB, FFPW). Spawning and fertilized egg production were obtained from pond-cultured pikeperch broodstock [29,30] (TL = 517 ± 35 mm and W = 1215 ± 200 g), held at the same facility under controlled conditions [31] in RAS. Used broodstock was regularly spawned since 2017, Final oocyte and sperm maturation was performed under spawned 15:9 h light:darkness regime with a light intensity of 100 lux, water temperature of 15 ± 0.5 • C [6,31,32], and synchronized with a dose of the intramuscular hormonal injection Human Chorionic Gonadotropin (hCG; Chorulon, Intervet International B.V.) at 500 IU.kg −1 , as previously cited by Křištan [33] and Blecha [31].…”

“…All broodstock were anesthetized with clove oil (Dr. Kulich Pharma Ltd., Hradec Králové, Czech Republic) at a concentration 30 mgL −1 [34], before manipulation. After hormonal treatment, pairs composed of both sexes were separated and stocked in RAS tanks for nest spawning, following the research method set forth by Malinovskyi [29,30]. After spawning, egg fertilization, and laying, broodstock were removed and eggs on the nest were incubated in each tank under a water temperature of 16 ± 0.5 • C for 8 days until hatching occurred [30].…”

Use of Lactic Acid Bacteria During Pikeperch (Sander lucioperca) Larval Rearing

“…The trial was run at the University of South Bohemia, Facility of Fisheries and Protection of Waters, Czech Republic (USB, FFPW). Spawning and fertilized egg production was from pond-cultured pikeperch broodstock [25,26] (TL = 517 ± 35 mm and W = 1215 ± 200 g) held at the same facility under controlled conditions [27] in RAS. Final oocyte and sperm maturation were performed under a 15 h:9 h light/darkness regime with a light intensity of 100 lux, and a water temperature of 15 ± 0.5 • C [27][28][29].…”

“…All broodstock were anesthetized with clove oil (Dr. Kulich Pharma Ltd., Hradec Králové, Czech Republic) at a concentration of 30 mgL −1 [31] before manipulation. After hormonal treatment, pairs of both sexes were separated and stocked in RAS tanks for nest spawning, as previously studied by Malinovskyi [25,26]. After spawning, egg fertilization, and laying, broodstock were removed and eggs on the nest were incubated in each tank under a water temperature of 16 ± 0.5 • C, for 8 days until hatching occurred [25].…”

“…After hormonal treatment, pairs of both sexes were separated and stocked in RAS tanks for nest spawning, as previously studied by Malinovskyi [25,26]. After spawning, egg fertilization, and laying, broodstock were removed and eggs on the nest were incubated in each tank under a water temperature of 16 ± 0.5 • C, for 8 days until hatching occurred [25]. Three-day old larvae were stocked at 100 larvae per liter into 2 L larval rearing tanks (n = 12).…”