The substrate-dependent regeneration capacity of mesenchymal stem cell spheroids derived on various biomaterial surfaces

Huang, Guo-Shiang; Hsieh, Pai‐Shan; Tseng, Ching-Shiow; Hsu, Shan‐hui

doi:10.1039/c4bm00053f

Cited by 28 publications

(30 citation statements)

References 34 publications

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“…Besides, our previous studies demonstrated that MSC spheroids formed on PVA membranes had lower stemness and differentiation capabilities than those on chitosan [57]. Transplantation of PVA-derived spheroids in a rabbit chondral defect model did not significantly improve the cartilage regeneration, compared to chitosan-derived spheroids [58]. Therefore, we suggested that FOXD3-transfected spheroids obtained from PVA may not be as useful as FOXD3-transfected spheroids obtained from chitosan in repairing the neural deficit of the zebrafish.…”

Section: Discussionmentioning

confidence: 90%

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

et al. 2016

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Section: Discussionmentioning

confidence: 90%

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

et al. 2016

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“…MSCs grown on nonfouling hydrogel remained undifferentiated even after induction . Many hydrophilic material surfaces are nonadherent so cells are forced to form aggregates . On the current PU surface, cells attached prior to forming aggregates through cell assembly.…”

Section: Resultsmentioning

confidence: 93%

“…On the current PU surface, cells attached prior to forming aggregates through cell assembly. The self‐assembled aggregates are considered to be superior to forced aggregates . Moreover, the current PU can be conveniently processed for various biomedical applications .…”

Section: Resultsmentioning

confidence: 99%

Polymer Surface Interacts with Calcium in Aqueous Media to Induce Stem Cell Assembly

Hung

Hsu

2015

Adv Healthcare Materials

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Bioinspired surface with functional group rearrangement abilities are highly desirable for designing functional materials. Calcium ion (Ca(2+) ) is a pivotal life element and the ion transport is tightly regulated through calcium channels. It is demonstrated here that Ca(2+) can be transported by polymer surface to induce cell assembly. A series of polyurethane materials is synthesized with different abilities to rearrange the surface functional groups in response to aqueous environment. It is observed that surface recruitment of carboxyl and amino groups from the bulk material can interact with Ca(2+) and facilitate its translocation from aqueous media into cells. The surface rearrangement of functional group triggers the calcium trafficking and turns on signals involving cell merging and assembly. This observation provides an insight on adjusting material-calcium interaction to design nature-inspired smart interfaces to induce cell organization and tissue regeneration.

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“…Intercellular cadherin interaction regulates the cell-cell adhesion, cell migration, and multiple types of cell signaling by association with the actin filaments [72]. The up-regulated expression levels of N-cadherin and/or E-cadherin were observed in MSC spheroids formed by different scaffold-free 3D culture systems including hanging drop method and CS substrates, comparing to those cultured on TCPS [71,73], indicating the involvement of cadherin-mediated signaling in the formation of MSC spheroids. Meanwhile, the calcium ions were chelated by the CS substrates [73].…”

Section: Physical and Chemical Factors Affecting Cell Behaviors In Scmentioning

confidence: 93%

“…The up-regulated expression levels of N-cadherin and/or E-cadherin were observed in MSC spheroids formed by different scaffold-free 3D culture systems including hanging drop method and CS substrates, comparing to those cultured on TCPS [71,73], indicating the involvement of cadherin-mediated signaling in the formation of MSC spheroids. Meanwhile, the calcium ions were chelated by the CS substrates [73]. Combined with several findings mentioned above, we propose a mechanism of MSC spheroid formation on CS substrates.…”

Section: Physical and Chemical Factors Affecting Cell Behaviors In Scmentioning

confidence: 96%

Cellular Spheroids of Mesenchymal Stem Cells and Their Perspectives in Future Healthcare

2019

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Intrinsic cellular properties of several types of cells are dramatically altered as the culture condition shifts from two-dimensional (2D) to three-dimensional (3D) environment. Currently, several lines of evidence have demonstrated the therapeutic potential of mesenchymal stem cells (MSCs) in regenerative medicine. MSCs not only replenish the lost cells, they also promote the regeneration of impaired tissues by modulating the immune responses. Following the development of 3D cell culture, the enhanced therapeutic efficacy of spheroid-forming MSCs have been identified in several animal disease models by promoting differentiation or trophic factor secretion, as compared to planar-cultured MSCs. Due to the complicated and multifunctional applications in the medical field, MSCs are recently named as medicinal signaling cells. In this review, we summarize the predominant differences of cell-environment interactions for the MSC spheroids formed by chitosan-based substrates and other scaffold-free approaches. Furthermore, several important physical and chemical factors affecting cell behaviors in the cell spheroids are discussed. Currently, the understanding of MSCs spheroid interactions is continuously expanding. Overall, this article aims to review the broad advantages and perspectives of MSC spheroids in regenerative medicine and in future healthcare. Three-Dimensional (3D) Cell Culture SystemsCell-cell and cell-environment interactions cooperatively determine the physiological phenomena in vivo. Many cell growth mechanisms and signal transduction pathways occurring in cells have been unveiled by the traditional two-dimensional (2D) culture system. Plastic-based culture materials remain the predominant cell culture platforms to date, yet some concerns have been raised with the accumulating experimental evidence.A major drawback of cell culture on 2D plastic plates is the planar cell-cell interaction that has the insufficient and inappropriate cell-environment crosstalk. In vivo, cells are grown and stacked within a three-dimensional (3D) space, and extracellular matrix (ECM) secreted by cells constitutes the supporting framework to further organize tissues and organs. Therefore, some important cell-cell and cell-environment interactions may be ignored in a planar culture platform. Primary cells, cancer cells, and stem cells have been cultured in a 3D environment, and many interesting findings have been uncovered. In general, the definition of 3D cell culture systems is that cells are cultured on/in the 3D scaffolds composed by natural or synthetic materials, or they are organized into cellular spheroids. With the continuing development of 3D cell culture, it is now generally agreed that the cell physiology and cell behavior are dramatically distinct for cells cultured within a 2D or 3D environment [1][2][3].Cancer cells are the most common cell types that have been investigated by 3D culture systems, and obvious change of gene expression as well as intracellular signaling in these 3D-cultured cancer cells have been ...

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The substrate-dependent regeneration capacity of mesenchymal stem cell spheroids derived on various biomaterial surfaces

Abstract: ADSC spheroids derived on various biomaterials present different in vitro properties, which may explain their different efficacies in cartilage repair.

Cited by 28 publications

References 34 publications

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

Polymer Surface Interacts with Calcium in Aqueous Media to Induce Stem Cell Assembly

Cellular Spheroids of Mesenchymal Stem Cells and Their Perspectives in Future Healthcare

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