The Study on the Relationship between Leaf Colors and ptDNA Inheritance in Intersectional Cross of Rhododendron kiusianum×R. japonicum f. flavum, Resulting in an Unexpected Triploid Progeny

Ureshino, Kenji; Miyajima, Ikuo

doi:10.2503/jjshs.71.214

Cited by 20 publications

(10 citation statements)

References 19 publications

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“…PtDNA of most of BC 1 seedlings were inherited from the seed parent, evergreen azaleas, whereas those seedlings had a green leaf color. Ureshino and Miyajima (2002) reported that plastomegenome incompatibility between the plastome from evergreen azaleas and the nuclear genome from R. japonicum could be overcome by gene dosage of the nuclear genome from evergreen azaleas, i. e., the nuclear genome construction of seedlings was 2x evergreen azaleas-1x R. japonicum. Sakai et al (2004) also overcame hybrid albinism through an inter-ploidy cross of 4x evergreen azaleas × 2x R. japonicum f. flavum.…”

Section: Discussionmentioning

confidence: 99%

Cross Compatibility of Intersubgeneric Hybrids of Azaleas on Backcross with Several Evergreen Species

Ureshino¹,

Tashiro²,

Takeda³

et al. 2006

J. Japan. Soc. Hort. Sci.

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Section: Discussionmentioning

confidence: 99%

Cross Compatibility of Intersubgeneric Hybrids of Azaleas on Backcross with Several Evergreen Species

Ureshino¹,

Tashiro²,

Takeda³

et al. 2006

J. Japan. Soc. Hort. Sci.

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“…Inc., Siga, Japan). For analysis of cpDNA, three primer pairs of rbcL-PSA I, TrnD-TrnT and trnK-3914F-trnK-2R were used for amplification according to the methods of Cheng et al (2002) and Ureshino and Miyajima (2002). For analysis of mtDNA, three primer pairs of 18S rRNA-5S rRNA, nad 4 exon 1-nad 4 exon 2 and nad 7/1-nad 7/2r were used for amplification according to the method of Cheng et al (2002) and Dumolin-Lapegue et al (1997).…”

Section: Extraction Of Total Dnamentioning

confidence: 99%

Utilization of intergeneric somatic hybrids as an index discriminating taxa in the genus Citrus and its related species

et al. 2005

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Embryogenic protoplasts of 'Shogun' mandarin (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from Citropsis gabunensis Swing. & M. Kell, and two green embryoids were regenerated after 3 months of culture. Two months after transfer to the regeneration medium, numerous plantlets were obtained from the embryoids. These plants grew vigorously, had well-developed root systems, and exhibited leaf characteristics intermediate to those of the parents. The absolute nuclear genome size of the regenerated plant SH2 (1.75 pg/4C) was the sum of those of the 'Shogun' mandarin (0.75 pg/2C) and C. gabunensis (0.97 pg/2C). The chromosome counts of the young leaves revealed that they were tetraploids (2n=4x=36). Random amplified polymorphic DNA (RAPD) analysis of the two lines (SH1 and SH2) verified their hybridity. Cytoplasmic genome analysis using universal primers reveal that their chloroplast (cp) DNA banding pattern is identical to that of C. gabunensis, while the banding pattern of the mitochondrial (mt) DNA is identical to that of the 'Shogun' mandarin. These somatic hybrids are important materials for investigating the phylogenetic relationships between these two genera in the subfamily Aurantioideae.

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“…the incompatibility was overcome in the cross of 4x EV × 2x JP (Sakai et al, 2008;Ureshino et al, 2002). From the results, as a reason for overcoming the incompatibility, two hypotheses could be considered, i.e.…”

Section: Introductionmentioning

confidence: 99%

Relationship between Nuclear Genome Construction and the Plastome-genome Incompatibility of Progenies from Intra- and Inter-ploid Cross of Evergreen Azaleas × Rhododendron japonicum f. flavum

Ureshino

Abe

Akabane³

2010

J. Japan. Soc. Hort. Sci.

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In intersubgeneric crosses of diploid evergreen azaleas (EV) × diploid Rhododendron japonicum f. flavum (JP), many progenies become albino as a result of plastome-genome incompatibility between a plastid genome from EV and a nuclear genome from JP. To clarify the mechanism of the incompatibility, inter-and intra-ploid crosses of diploid (2x) EV × diploid (2x) JP, tetraploid (4x) EV × tetraploid (4x) JP, 2x EV × 4x JP, and 4x EV × 2x JP were conducted. Seeds were obtained in all crosses, although the number of progenies per capsule was relatively low in crosses with 4x JP as a pollen parent. Plastome-genome incompatibility between a plastid genome from EV and a nuclear genome from JP was observed in progenies from 2x EV × 2x JP, 4x EV × 4x JP, and 2x EV × 4x JP, but not in 4x EV × 2x JP. This result indicates that plastome-genome incompatibility was broken by relative increase of the genome from EV in the nuclear genome of progenies.

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The Study on the Relationship between Leaf Colors and ptDNA Inheritance in Intersectional Cross of Rhododendron kiusianum×R. japonicum f. flavum, Resulting in an Unexpected Triploid Progeny

Cited by 20 publications

References 19 publications

Cross Compatibility of Intersubgeneric Hybrids of Azaleas on Backcross with Several Evergreen Species

Cross Compatibility of Intersubgeneric Hybrids of Azaleas on Backcross with Several Evergreen Species

Utilization of intergeneric somatic hybrids as an index discriminating taxa in the genus Citrus and its related species

Relationship between Nuclear Genome Construction and the Plastome-genome Incompatibility of Progenies from Intra- and Inter-ploid Cross of Evergreen Azaleas × Rhododendron japonicum f. flavum

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