The study on mechanical properties of Phytagel medium

Abstract

Background: The mechanical properties of plant culture medium such as Phytagel affect plant growth and development. Given that the mechanical properties of Phytagel medium are vital for biomechanical experiments designing, a systematic study on mechanical properties of Phytagel medium with different concentrations were carried out here to better understand the response of plant to mechanical stimulation. Results: Uniaxial compression test was conducted for the mechanical strength and Young’s modulus. The variation of concentrations of media results in different mechanical strength. The linear-regression analysis of the breaking load shows that there is a lack of fit of the linear regression model to the observed data points for all these Phytagel concentrations (R2 = 0.9708). The spline regression model, however, fits well to the Young’s modulus for Phytagel medium data. The rheological measurements from the oscillation tests (frequency sweep from 0.1 to 20 Hz at 1% strain) indicate that increasing the Phytagel concentrations results in a stiffer structure at 0.5%-1.2%. Conclusions: Although the biological effects of Phytagel on plant along with mechanical power are expected to check, this contribution provides a useful reference in biomechanical experiments to choose the best Phytagel concentration for the culture of plants and tissues.

Influence of Media Composition and Genotype on Potato (Solanum tuberosum L.) Microtuberization

“…Successful plant tissue culture depends on properties of growth medium, especially the mechanical property. Phytagel belongs to a complex fluid (Zhou et al, 2019). The addition of phytagel in media clearly support number of microtubers and weight of microtubers produced.…”

“…This may be due to present of glucose in phytagel together with sucrose in the media, induced microtuber production. Phytagel was commonly added at 0.5% -2.0% for optimum plant growth in vitro (Zhou et al, 2019). In this experiment, phytagel provided support for the shoots to grow upward and performed photosynthesis in the first four weeks, followed by microtuber formation for six weeks.…”

ZeBraInspector, a platform for the automated segmentation and analysis of body and brain volumes in whole 5 dpf zebrafish following simultaneous visualization with identical orientations

“…This mounting strategy is allowed by the fact that EE in high RI medium do not dessicate as fastly as if they were in water. EE are immobilized with a drop of phytagel, a transparent plant culture medium (Zhou et al, 2019) which serendipitously proved to be ideal for microscopy. EE can be placed with the dorsal or ventral side up, depending on the region of the brain to analyze.…”

Root discoloration and shoot symptoms in silver birch afterPhytophthorainfectionin vitro

“…In total, 57 birch seedlings were cultivated as described above except that agar was replaced with Phytagel (Zhou et al . 2019). The Phytophthora treatment included 40 seedlings, with 17 seedlings left as controls.…”

“…A separate experiment was established to investigate the effects of Phytophthora on the shoots of silver birch seedlings. In total, 57 birch seedlings were cultivated as described above except that agar was replaced with Phytagel (Zhou et al 2019). The Phytophthora treatment included 40 seedlings, with 17 seedlings left as controls.…”