The Study on Biological Function of Keratin 26, a Novel Member of Liaoning Cashmere Goat Keratin Gene Family

Jin, Mei; Wang, Jing; Chu, Mingxing; Piao, Jinhua; Piao, Jinhua; Zhao, Fengqin

doi:10.1371/journal.pone.0168015

Cited by 18 publications

(12 citation statements)

References 49 publications

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“…RNA-seq is usual and convenient for researchers to study different animal phenotypes due to differentially expressed genes (DEGs), because we could get enough transcriptome data from small quantities of tissue samples[ 6 , 7 ]. In previous studies of hair follicle in Cashmere goats, the scientists have already found many important factors that may affect hair follicle cycle in Wnt signal transduction pathway, fibroblast growth factor (FGF) family, bone morphogenetic protein (BMP) family, Sonichedgehog (Shh) signal transduction pathway, transforming growth factor (TGF) family, Notch signal transduction pathway and so on [ 8 , 9 , 10 ]. Some genes play an activator role while other genes play roles in these pathways as the inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis reveals critical genes for the hair follicle of Inner Mongolia cashmere goat from catagen to telogen

Fan

Qiao

et al. 2018

PLoS ONE

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There are two main types of hair follicle in Inner Mongolia Cashmere goats, the primary hair follicle (PHF) producing hair fibers and the secondary hair follicle (SHF) producing cashmere fibers. Of both fibers from cashmere-bearing goats in Aerbasi, Inner Mongolia, the timing of cyclical phases for the cashmere have been well clarified but hair fibers have been less noticeable. Herein, we evaluated transcriptome of PHF and SHF from the same three goats in Aerbasi at the catagen- and telogen phase of cashmere growth. We totally found 1977 DEGs between PHFs at the telogen and catagen phases of SHF, 1199 DEGs between telogen- and catagen SHF, 2629 DEGs between PHF at the catagen phase of SHF and catagen SHF, and 755 DEGs between PHF at the telogen phase of SHF and telogen SHF. By analyzing gene functions based on GO and KEGG database, we found that the DEGs have functions in muscle contraction and muscle filament sliding between catagen- and telogen SHF, indicating that arrector pilli muscles might play a role on the transition from catagen to telogen. Moreover, considering that the enriched GO and KEGG categories of the DEGs between PHF and SHF, we suggested that part of PHF might rest in their own anagen phase when SHF are at catagen, but PHF might enter into the telogen phase at SHF’s telogen. Finally, we highly recommended the several potential genes acting as the regulators of the transition between growth phases including IL17RB and eight members of ZNF. These results provide insight into molecular mechanisms on the transition of SHF from catagen to telogen together with PHF’s growth situation at SHF’s catagen and telogen in Inner Mongolia Cashmere goats.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis reveals critical genes for the hair follicle of Inner Mongolia cashmere goat from catagen to telogen

Fan

Qiao

et al. 2018

PLoS ONE

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“…KRT and KRTAPs contribute nearly 90% of the cashmere yield [38][39][40], demonstrating indirectly that the composition and interactions of these proteins play an important role in cashmere quality [41][42][43].…”

Section: Discussionmentioning

confidence: 99%

Skin transcriptome reveals the Periodic changes in genes underlying hair follicle cycling in Cashmere goats

Liu

Yang

Zhao

et al. 2019

Preprint

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Background Cashmere goats, as an important part of animal husbandry production, make outstanding contributions to animal fiber industry. In recent years, a great deal of research has been done on the molecular regulation mechanism of hair follicle cycle growth. However, there are few reports on the molecular regulation mechanisms of secondary hair follicle growth cycle in cashmere goats. In order to explore the changing regularity of wool follicle cycle and the role of key genes in wool follicle cycle of cashmere goats. we used transcriptome sequencing technique to sequence the skin of Inner Mongolia cashmere goats in different periods, Analyze the variation and difference of genes in the whole hair follicle cycle. And then, we verified the regulation mechanism of cashmere goat secondary hair follicle growth cycle by fluorescence quantitative PCR. Results As the result shows: growth period (March-September), regression period (September-December) and resting period (December-March). The results of differential gene analysis showed that March was considered the beginning of the cycle, and the difference of gene expression was the most significant. Cluster analysis of gene expression in the whole growth cycle further supported the key nodes of the three periods of villus growth, and the differential gene expression of keratin corresponding to the villus growth cycle further supported the results of tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1 and KRTAP 24-1 genes had close positive correlation with the growth cycle of cashmere, and their regulation was consistent with the growth cycle of cashmere. However, there was a sequence of expression time, indicating that the results of cycle regulation made the growth of cashmere change. Conclusion The growth cycle of cashmere hair could be divided into three distinct periods: growth period (March-September), regression period (September-December) and resting period (December-March).March was considered the beginning of the cycle, KAP and KRTAP had close positive correlation with the growth cycle of cashmere, and their regulation was consistent with the growth cycle of cashmere. However, there was a sequence of expression time, indicating that the results of cycle regulation made the growth of cashmere change.

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“…KRT and KRTAPs together make up nearly 90% of the cashmere yield [38][39][40], demonstrating indirectly that the composition and interactions of these proteins play an important role in cashmere quality [41][42][43]. KRT and KRTAP gene expression directly affect the fineness and density of cashmere as well as other characteristics [44].…”

Section: Discussionmentioning

confidence: 99%

Skin transcriptome reveals the periodic changes in genes underlying cashmere (ground hair) follicle transition in cashmere goats

et al. 2020

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Background: Cashmere goats make an outstanding contribution to the livestock textile industry and their cashmere is famous for its slenderness and softness and has been extensively studied. However, there are few reports on the molecular regulatory mechanisms of the secondary hair follicle growth cycle in cashmere goats. In order to explore the regular transition through the follicle cycle and the role of key genes in this cycle, we used a transcriptome sequencing technique to sequence the skin of Inner Mongolian cashmere goats during different months. We analyzed the variation and difference in genes throughout the whole hair follicle cycle. We then verified the regulatory mechanism of the cashmere goat secondary hair follicle growth cycle using fluorescence quantitative PCR. Results: The growth cycle of cashmere hair could be divided into three distinct periods: a growth period (March-September), a regression period (September-December), and a resting period (December-March). The results of differential gene analyses showed that March was the most significant month. Cluster analysis of gene expression throughout the whole growth cycle further supported the key nodes of the three periods of cashmere growth, and the differential gene expression of keratin corresponding to the ground haircashmere growth cycle further supported the results from tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1, and KRTAP 24-1 genes had close positive correlation with the cashmere growth cycle, and their regulation was consistent with the growth cycle of cashmere.

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The Study on Biological Function of Keratin 26, a Novel Member of Liaoning Cashmere Goat Keratin Gene Family

Cited by 18 publications

References 49 publications

Transcriptomic analysis reveals critical genes for the hair follicle of Inner Mongolia cashmere goat from catagen to telogen

Transcriptomic analysis reveals critical genes for the hair follicle of Inner Mongolia cashmere goat from catagen to telogen

Skin transcriptome reveals the Periodic changes in genes underlying hair follicle cycling in Cashmere goats

Skin transcriptome reveals the periodic changes in genes underlying cashmere (ground hair) follicle transition in cashmere goats

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