The Study of the Higher Aquatic Flora of the Middle Don Basin within Rostov Region

Sereda, Michael M.; Kapralova, Olga A.; Sereda, Vlada A.; Kozlovskiy, B. L.; Лысенко, В. С.; Varduni, Tatiana V.; Dmitriev, Pavel; Lysenko, Olesya G.

doi:10.3844/ojbsci.2015.244.250

Cited by 1 publication

(1 citation statement)

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“…The average values of the shoots per explant when using the DKW environment were lower than those for the MS. A significant excess of the shoots per explant when using the MS medium compared with the DKW medium. As a result of the combined action of BAP and IAA in MS and DKW media, the data obtained are consistent with the literature data that combinations of various growth regulators often have a high synergistic effect [30,31]. In most cases, the average values of the length of H. cretaceum shoots on different media are consistent with the values of the shoots per explant.…”

Section: Discussionsupporting

confidence: 89%

An Efficient Method for Micropropagation of Red-List Herbaceous Plant Species (Hedysarum cretaceum)

Chokheli¹,

Bakulin²,

Ermolaeva³

et al. 2023

OBM Genet

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Hedysarum cretaceum Fisch is a critically endangered herbaceous species with high ornamental and phytomeliorative properties; thus, it needs urgent conservation and protection methods to conserve. In the present study, an efficient method for microtonal propagation of H. cretaceum is developed. The seed sterilization was optimized as treatment with 96% alcohol (2 min) and burned (1 sec) through a burner flame. Murashige and Skoog (MS) medium with the addition of 0.5 mg/L 6-Benzylaminopurine (BAP) (shoots per explant – 18.0) and 1.0 mg/L BAP and 0.1 mg/L Indole-3-acetic acid (IAA) (shoots per explant – 18.4) for the shoot and ⅓ MS supplemented with 0.1 mg/L IAA (rooting indicator – 62%) for roots were identified best composition for the proliferation and regeneration. Additionally, the medium for maintaining callus culture was determined – DKW with the addition of 2.5 mg/L BAP and 0.1 mg/L IAA, the possibility of direct organogenesis in vitro in the studied plant was also standardized. In addition, seed germination after various pre-sowing treatments was studied. It is shown that scarification makes achieving 80% germination of seeds possible. Also, sulfuric acid was used as a pre-sowing seed treatment, germination was 45%. Thus, using the protocols we developed, it is possible to maintain the H. cretaceum culture in vitro and increase seed germination using pre-sowing treatment.

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Section: Discussionsupporting

confidence: 89%