The study of surface reactions in biological systems by ellipsometry

Poste, George; Moss, C. A.

doi:10.1016/0079-6816(72)90009-3

Cited by 36 publications

(12 citation statements)

References 165 publications

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“…The mechanism by which microexudates derived from exponentially growing cell cultures enhance cell growth at low densities remains to be established. The use of EDTA or trypsin, or both, to detach cells from substrata results in the removal or peripheral components from cells (6 8, 17, 20, 22, 38) that are resynthesized over a period of 1 8 h (2,13,15,17,19,25,26). The removal of these peripheral components creates abnormal permeability fluxes across the plasma membrane, and cells remain "leaky" until the surface components are resynthesized.…”

Section: Discussionmentioning

confidence: 99%

“…Mammalian cells cultured in vitro on solid substrates produce a thin layer of "microexudate" that remains attached to the substrate when the cells are removed. This material is not detected by light microscopy, but can be demonstrated by electron microscopy (1,3,5,14,27,31,37,40,45), ellipsometry (25,32,34), mixed hemadsorption (26,42), immunofluorescence techniques (3,26,43), and the incorporation of radioisotopes (8,34,44,45). The microexudates deposited on substrates by mammalian cells are actively synthesized by the cell and do not result merely from the passive leakage of intracellular macromolecules onto the substrate (18,25).…”

mentioning

confidence: 99%

“…The microexudates deposited on substrates by mammalian cells are actively synthesized by the cell and do not result merely from the passive leakage of intracellular macromolecules onto the substrate (18,25). The demonstration of cellspecific antigens (3,26,42,43), virus receptors (25), and lectin-binding sites (23,25) in cellular microexudates, together with data on the synthesis (25) and chemical characterization of such materials (8), have prompted the proposal that microexudates represent cell surface macromolecules derived from the so-called "cell coat" (25).…”

mentioning

confidence: 99%

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Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities.

Weiss¹,

Poste²,

MacKearnin³

et al. 1975

The Journal of Cell Biology

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Mammalian and avian cells cultured on glass or plastic substrates produce microexudates of cellular macromolecules which remain bound to the substrate when the cells are detached. The gross macromolecular composition of microexudates from a range of diploid, heteroploid, and virus-transformed cells was determined with cells labeled with radioisotopes. Significant differences in the amounts of cellular glycoproteins, proteins, and RNA present in microexudates were found between different cell types and between cells of the same type at different stages of growth. Inoculation of cells onto substrates "coated" with microexudates altered their growth behavior. Microexudates from exponentially growing subconfluent homotypic and heterotypic cell populations enhanced the growth of mouse and chick embryo cells seeded at very low densities, but similar microexudates had no effect on the proliferation of cells seeded at higher densities. The enhanced growth of low-density cell populations seeded on microexudates was compared with the growth enhancement produced by feeder cell layers and conditioned medium.Mammalian cells cultured in vitro on solid substrates produce a thin layer of "microexudate" that remains attached to the substrate when the cells are removed. This material is not detected by light microscopy, but can be demonstrated by electron microscopy (1, 3, 5, 14, 27, 31, 37, 40, 45), ellipsometry (25, 32, 34), mixed hemadsorption (26, 42), immunofluorescence techniques (3,26,43), and the incorporation of radioisotopes (8,34,44,45). The microexudates deposited on substrates by mammalian cells are actively synthesized by the cell and do not result merely from the passive leakage of intracellular macromolecules onto the substrate (18,25). The demonstration of cellspecific antigens (3,26,42,43), virus receptors (25), and lectin-binding sites (23,25) in cellular microexudates, together with data on the synthesis (25) and chemical characterization of such materials (8), have prompted the proposal that microexudates represent cell surface macromolecules derived from the so-called "cell coat" (25).In this paper we present information on the composition of cellular microexudates deposited on glass and plastic substrates by a variety of diploid, heteroploid, and transformed cells and on alterations in the growth behavior of cells plated at

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities.

Weiss¹,

Poste²,

MacKearnin³

et al. 1975

The Journal of Cell Biology

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“…The method has proven to be well suited for in situ investigations of a variety of adsorption/desorption processes (Trurnit, 1953;Vroman & Adams, 1969;Poste & Moss, 1972;Arnebrant, 1987;Lundström et al, 1987;Nyländer, 1987). Studies regarding adsorption of human saliva at different solid/liquid interfaces by this technique have previously been reported in the literature (Ericson et al, 1982;Vassilakos et al, 1992).…”

Section: Introductionmentioning

confidence: 95%

Concentration dependence of adsorption from human whole resting saliva at solid/liquid interfaces: An ellipsometric study

Lindh¹,

Arnebrant

Isberg

et al. 1999

Biofouling

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The rates and plateau values of adsorption at the solid/ liquid interface from human whole resting saliva from three male donors were studied in vitro. The influence of saliva concentration, the total protein content and the wettability of the exposed solids was investigated. Ellipsometry was used to study the rate and plateau values of the adsorption process in situ. The solid surfaces used were hydrophilic, negatively charged, silica and hydrophobic methylated silica. Salivary films were formed by adsorption from solutions of 0.05%, 0.10%, 0.20%, 0.50%, 1.0% and 2.0% (v/v) saliva in phosphate buffered saline. The results revealed that adsorption onto both types of surfaces was a rapid and, as expected, a concentration dependent process. The adsorbed amounts were in the range of monomolecular layers and were found to be larger onto the hydrophobic than the hydrophilic surfaces (p 0.0062). A strong concentration dependence was denoted in all cases (p< 0.0001). Inter-individual differences were found not to be significant (0.0636 p 0.8508) at any of the saliva concentrations. However, an interaction was shown (p < 0.0001) between the protein concentrations and the donors, indicating qualitative differences in protein content and surface activity.

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“…In the life sciences, there were a few pioneering works using ellipsometry for immunological studies and for measuring the kinetics of protein adsorption. A review of these works from 1972 [22] demonstrated the use of ellipsometry in only a small number of applications. Over a decade later, some of the first quantitative comparisons, between ellipsometry and radiolabeling techniques, of the surface mass density of adsorbed layers of proteins were demonstrated [23].…”

Section: Introductionmentioning

confidence: 99%

Kinetic Analysis of Glycoprotein–Lectin Interactions by Label-Free Internal Reflection Ellipsometry

et al. 2008

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Introduction Glycoproteomics is undergoing rapid development, largely as a result of advances in technologies for isolating glycoproteins and analyzing glycan structures. However, given the number and diversity of glycans, there is need for new technologies that can more rapidly provide differential carbohydrate-protein structural information on a large scale. We describe a new microarray platform based on a label-free imaging ellipsometry technique, which permits simultaneous detection of multiple glycoproteinlectin interactions without the need for reporter labels, while still providing high throughput kinetic information at much lower cost. Our results demonstrate the utility of LFIRE™ (Label-Free Internal Reflection Ellipsometry) for the rapid kinetic screening of carbohydrate-lectin recognition. The technology was also used to evaluate the benefits of the lectin immobilization format using multi-lectin affinity chromatography (M-LAC) to capture glycoproteins (with enhanced binding strength or avidity) from biological samples. Using a printed panel of lectins, singly or in combination, we examined the binding characteristics of standard glycoproteins.Results and Discussion Using kinetic measurements, it was observed that the binding strength of lectins to carbohydrates is enhanced using a multi-lectin strategy, suggesting that improved selectivity and specificity can lead to increased functional avidity. The data presented confirm that this label-free technology can be used to effectively screen single or combinations of lectins. Furthermore, the combination of LFIRE™ and M-LAC may permit more rapid and sensitive identification of novel biomarkers based on carbohydrate changes in glycoproteins, and lead to a better understanding of the connections of glycan function in cellular mechanisms of health and disease.

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The study of surface reactions in biological systems by ellipsometry

Cited by 36 publications

References 165 publications

Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities.

Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities.

Concentration dependence of adsorption from human whole resting saliva at solid/liquid interfaces: An ellipsometric study

Kinetic Analysis of Glycoprotein–Lectin Interactions by Label-Free Internal Reflection Ellipsometry

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