The Study of Ozone Ointment on Human Gingival Fibroblasts Cell Proliferation Ability and Anti-Inflammatory

Abstract

Ozone is currently being considered as a possible oral antiseptic agent because it is strongly antimicrobial and does not induce microbial resistance. Here, we examined the eff ects of ozone exposure on the production of collagen type-1 and infl ammatory cytokines in primary human gingival fi broblasts (HGFs) in vitro using enzyme-linked immunosorbent assays. In this study, we demonstrated that ozone ointment increased type 1 collagen production and hindered pro-infl ammatory cytokine secretion from primary HGFs in vitro. HGFs were isolated from a 65-year-old patient who had undergone surgery due to chronic periodontitis. The cells were exposed to media with or without 0.05, 0.5 and 5 ppm ozone ointment for 24 hours 2 min. No cytotoxic eff ect of the ozone ointment was observed up to the concentration of 0.5 ppm, cell viability was attenuated at the dose of 5 ppm. When ozone ointment was used at the non-cytotoxic concentration of 0.5 ppm, it signifi cantly enhanced type 1 collagen production by HGFs within for 24 hours. Secretion of the pro-infl ammatory cytokines interleukin (IL)-6 and IL-8 by HGFs treated with lipopolysaccharide (LPS) decreased when ozone ointment was present in the medium. These results suggest that the therapeutic eff ect of ozone ointment against periodontal disease is partially due to modulation of the function of HGFs.

Ozonioterapia como coadjuvante no tratamento da periodontite: revisão de literatura

“…Com foco na periodontia, estudos pré-clínicos demonstraram resultados satisfatórios desde as primeiras etapas do processo científico. Os efeitos in vitro foram apresentados nos estudos de Huth, et al (2007); Wang. et al (2018) Em sequência, às etapas do processo científico, estudos em animais devem ser realizados para comprovar os efeitos terapêuticos.…”

“…The Study of Ozone Ointment on Human Gingival Fibroblasts Cell Proliferation Ability and Anti-Inflammatory Wang., et al, 2018 1. Verificar os efeitos da exposição ao ozônio em pomada na produção de colágeno.…”

Latest advancement in periodontology

“…33 Examination is being done in the field of nanotechnology for delivering better and even adjusted items for periodontal recovery with the end of reactions and expanding the biocompatibility of the item. 34,35…”

Comparative Study of Ozonated Glycerol and Macrogol Ointment on Bone Matrix Production by Human Osteosarcoma Cell Line Saos-2

“…To apply ozonated glycerol for clinical use, ozonated macrogol ointment has been developed by adding macrogol ointment to ozonated glycerol to increase retention in the affected area. We have shown the anti-inflammatory effect of ozonated macrogol ointment on human gingival fibroblasts and its osteogenic potential on osteoblasts [ 15 ]. However, no studies have investigated the effects of macrogol addition in the ozonated gel.…”