The study of induced antimutagenesis of propionic acid bacteria

Vorobjeva, Lena I.; Khodjaev, E.Yu.; Cherdinceva, T.A.

doi:10.1016/0167-7012(95)00044-5

Cited by 13 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, Kumagai et al (1988) showed that the addition of L-cysteine and glycine to the fermentation medium induced GST activity in the yeast Issatchenkia orientalis. Vorobjeva et al (1996) and Zhang et al (2007) reported similar results. Placket-Burman data for GPx revealed top three significant factors including; sodium chloride, bile salt and urea.…”

Section: Discussionsupporting

confidence: 61%

Stress-Based Production, and Characterization of Glutathione Peroxidase and Glutathione S-Transferase Enzymes From Lactobacillus plantarum

Al-Madboly

Ali

Fakharany

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

More attention has been recently directed toward glutathione peroxidase and s-transferase enzymes because of the great importance they hold with respect to their applications in the pharmaceutical field. This work was conducted to optimize the production and characterize glutathione peroxidase and glutathione s-transferase produced by Lactobacillus plantarum KU720558 using Plackett-Burman and Box-Behnken statistical designs. To assess the impact of the culture conditions on the microbial production of the enzymes, colorimetric methods were used. Following data analysis, the optimum conditions that enhanced the s-transferase yield were the De Man-Rogosa-Sharp (MRS) broth as a basal medium supplemented with 0.1% urea, 0.075% H 2 O 2 , 0.5% 1-butanol, 0.0125% amino acids, and 0.05% SDS at pH 6.0 and anaerobically incubated for 24 h at 40 • C. The optimum s-transferase specific activity was 1789.5 U/mg of protein, which was ∼12 times the activity of the basal medium. For peroxidase, the best medium composition was 0.17% urea, 0.025% bile salt, 7.5% Na Cl, 0.05% H 2 O 2 , 0.05% SDS, and 2% ethanol added to the MRS broth at pH 6.0 and anaerobically incubated for 24 h at 40 • C. Furthermore, the optimum peroxidase specific activity was 612.5 U/mg of protein, indicating that its activity was 22 times higher than the activity recorded in the basal medium. After SDS-PAGE analysis, GST and GPx showed a single protein band of 25 and 18 kDa, respectively. They were able to retain their activities at an optimal temperature of 40 • C for an hour and pH range 4-7. The 3D model of both enzymes was constructed showing helical structures, sheet and loops. Protein cavities were also detected to define druggable sites. GST model had two large pockets; 185Å3 and 71 Å3 with druggability score 0.5-0.8. For GPx, the pockets were relatively Al-Madboly et al.Enzymes Production From Lactobacillus plantarum smaller, 71 Å3 and 32 Å3 with druggability score (0.65-0.66). Therefore, the present study showed that the consortium components as well as the stress-based conditions used could express both enzymes with enhanced productivity, recommending their application based on the obtained results.

show abstract

Section: Discussionsupporting

confidence: 61%

Stress-Based Production, and Characterization of Glutathione Peroxidase and Glutathione S-Transferase Enzymes From Lactobacillus plantarum

Al-Madboly

Ali

Fakharany

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Pr. shermanii was shown to have antimutagenic properties against 4-nitroquinoline-1-oxide (4NQO) which could be linked to the concomitantly enhanced GST activity as a result of exposure to the mutagen [ 50 ]. GSTs are known to degrade biphenyl, but no specific activity of GST in Lb.…”

Section: Distribution Of Glutathione In Selected Lab and Closely Relamentioning

confidence: 99%

Current status and emerging role of glutathione in food grade lactic acid bacteria

et al. 2012

View full text Add to dashboard Cite

Lactic acid bacteria (LAB) have taken centre stage in perspectives of modern fermented food industry and probiotic based therapeutics. These bacteria encounter various stress conditions during industrial processing or in the gastrointestinal environment. Such conditions are overcome by complex molecular assemblies capable of synthesizing and/or metabolizing molecules that play a specific role in stress adaptation. Thiols are important class of molecules which contribute towards stress management in cell. Glutathione, a low molecular weight thiol antioxidant distributed widely in eukaryotes and Gram negative organisms, is present sporadically in Gram positive bacteria. However, new insights on its occurrence and role in the latter group are coming to light. Some LAB and closely related Gram positive organisms are proposed to possess glutathione synthesis and/or utilization machinery. Also, supplementation of glutathione in food grade LAB is gaining attention for its role in stress protection and as a nutrient and sulfur source. Owing to the immense benefits of glutathione, its release by probiotic bacteria could also find important applications in health improvement. This review presents our current understanding about the status of glutathione and its role as an exogenously added molecule in food grade LAB and closely related organisms.

show abstract

“…This reinforces their potentialities as probiotics. The characterisation of a cytoplasmic protein, which exhibits reactivating effects on both prokaryotic and eukaryotic organisms [29,30], might further highlight the beneficial role of these bacteria in human alimentation [27].…”

Section: Introductionmentioning

confidence: 99%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

View full text Add to dashboard Cite

-Propionibacteria, which are largely used as Swiss-type cheese starters, also constitute promising human probiotics because of their beneficial effects on intestinal bifidobacteria and bacterial enzyme activities. To be efficient, they must survive acid stress imposed within the stomach and the surfactant effects of bile salts. We thus investigated the ability of Propionibacterium freudenreichii (strain Propiofidus ® SI41) to adapt to, and survive these stresses. In an in vivo study, 7 volunteers received Propiofidus ® SI41 in 3 different modes. The probiotic was supplied during 3 periods (i) in classical capsules at a low dose (5 × 10 9 CFU·d -1 ), (ii) in the same capsules at a higher dose (5 × 10 10 CFU·d -1 ), (iii) in acid-resistant capsules at the low dose. In the first case, propionibacteria were recovered above 10 5 CFU·g -1 in only 8 out of 14 faecal samples. In the two following cases, they were enumerated in all faecal samples between 10 5 and 10 7 CFU·g -1 . The recovery of live propionibacteria in faecal samples seemed to be related to a higher content in short chain fatty acids. This study, which clearly showed the role of gastric protection in the survival of propionibacteria, led us to investigate stress adaptation in this strain. Acid tolerance response was evidenced: transient exposure to pH 5 afforded protection towards acid challenge at pH 2. Moreover, we showed that pre-exposure to a moderate bile salts pre-treatment at 0.2 g·L -1 sharply increased its survival against a subsequent lethal challenge (1 g·L -1 ). Important changes in cellular protein synthesis were observed during adaptation using two-dimensional electrophoresis. Global protein synthesis decreased during adaptation treatments. In contrast, specific proteins were overexpressed during acid adaptation and others during bile salts adaptation. Among the up-regulated polypeptides, a carboxyl carrier protein (BCCP) and the recombinase RecR were identified during both stress responses while the universal chaperonins GroEL and GroES, as well as the replicase RepB, were only up-regulated during acid adaptation. These results bring new insights on the tolerance of P. freudenreichii to acid and bile salts, which should be taken into consideration for the development of probiotic preparations. Résumé -Survie et effets bénéfiques des bactéries propioniques dans l'intestin humain : étu-des in vivo et in vitro. Les propionibactéries, qui sont largement utilisées comme levain d'affinage des fromages à pâte pressée cuite, constituent également des probiotiques humains prometteurs du fait de leurs effets bénéfiques sur la flore bifide et les activités enzymatiques bactériennes, au niveau de l'intestin. Pour être efficaces, elles doivent survivre au stress acide subi dans l'estomac et à l'action détergente des sels biliaires. Nous avons donc étudié la capacité de Propionibacterium freudenreichii (souche Propiofidus ® SI41) à s'adapter et à survivre à ces stress. Dans une étude in vivo, 7 volontaires ont reçu Propiofidus ® SI41 selon 3 mo...

show abstract

The study of induced antimutagenesis of propionic acid bacteria

Cited by 13 publications

References 13 publications

Stress-Based Production, and Characterization of Glutathione Peroxidase and Glutathione S-Transferase Enzymes From Lactobacillus plantarum

Stress-Based Production, and Characterization of Glutathione Peroxidase and Glutathione S-Transferase Enzymes From Lactobacillus plantarum

Current status and emerging role of glutathione in food grade lactic acid bacteria

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Contact Info

Product

Resources

About