The study of genetic diversity in a minicore collection of durum wheat genotypes using agro-morphological traits and molecular markers

Shaygan, N.; Etminan, Alireza; Hervan, I. Majidi; Azizinezhad, Reza; Mohammadi, Reza

doi:10.1007/s42976-020-00073-6

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…Crossing of genotypes from these groups can increase the micronutrient concentration by combining the diverse genes controlling different morphological traits into single plant. Such recommendations in wheat breeding has been advocated by different researchers in past by [7,8,9,10]. Genotype HP-44, HP-49, HP-13 and HP-14 lying in different clusters can be ideal candidate lines for utilization in hybridization with adapted varieties namely, HD-3086, RSP-561 and JAUW -683 provided they have high zinc and iron content.…”

Section: Resultsmentioning

confidence: 99%

Genetic Divergence in Micronutrient Rich wheat– A Tool to Identify Diverse Parents

Sheera

Dey

Pandey

et al. 2022

IJECC

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Aim: To Study genetic divergence in the micronutrient rich lines to identify diverse parents for hybridization. Study Design: Randomized Complete Block Design Place and Duration of Study: Division of Plant Breeding and Genetics, She-e-Kashmir University of Agricultural Sciences and Technology of Jammu during rabi 2019-20. Methodology: Nature and magnitude of variability in forty-nine zinc/iron rich genotypes along with three commercial varieties with stripe rust resistance could be grouped into five clusters using D2–Statistics. Results: Fifty-two lines were placed in five cluster with most of the micronutrient rich lines lying in cluster I except for HP-44 , HP 49 , HP 14 and HP 13 placed in cluster II, III, IV and V respectively indicating them to be divergent than the rest. Further HD 3086, RSP 561 and JAUW-683 were in the cluster I indicating them to be less diverse with respect to traits under study. The traits that contributed mast towards divergence were grain yield per plant(25) percent followed by number of tillers per plant (20.7) percent and 1000 grain weight (14) percent zinc and iron add little contribution of 8 and 7 percent respectively to the total divergence hence could not classified the genotypes into different cluster based on inter cluster distance Cluster III and Cluster V had the greatest inter-cluster distance, followed by Cluster III and Cluster IV, Cluster II and Cluster V, and Cluster II and Cluster IV. Conclusion: Developing wheat varieties with enhanced content of iron and zinc is one of the most sought objectives in a present world to alleviate micronutrient malnutrition. Modern wheat varieties exhibit little diversity in zinc and iron levels in the grain, but large-scale screening has found significant amounts of zinc and iron in wild relatives and progenitors of cultivated wheat.

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Section: Resultsmentioning

confidence: 99%

Genetic Divergence in Micronutrient Rich wheat– A Tool to Identify Diverse Parents

Sheera

Dey

Pandey

et al. 2022

IJECC

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“…Cluster analysis was performed using MEGA 6.0 software. First, the Jaccard's dissimilarity distance matrix was calculated using DARWIN software version 6.0 (Shaygan et al 2021). Afterward, the cluster dendrogram was obtained using the neighbor-joining method based on the distance matrix.…”

Section: Discussionmentioning

confidence: 99%

“…Signi cant polymorphisms and the formation of more than one band were among the practical factors in obtaining acceptable results. Some other kinds of FMs, such as CAAT box-derived polymorphism primers, have been used successfully to evaluate the genetic diversity of durum wheat (Shaygan et al 2021;Etminan et al 2019;Khodaee et al 2021;Ghobadi et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Rainfed Durum Wheat Genotypes Based On Functional Markers Associated With Grain Quality Proteins

Kheibary

Azizinezhad

Mehrabi

et al. 2021

Preprint

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Analysis of genetic diversity provides helpful information necessary to develop the breeding and conservation strategies of crops. In this study, the genetic diversity and population structure of 90 durum wheat genotypes maintained at Sararud Dryland Agricultural Research Institute, Kermanshah, Iran, were evaluated by using 23 gene-specific markers (functional markers, FMs) encoding high and low molecular weight glutenin and gliadin alleles. Results showed that 12 out of the 23 FMs used were polymorphic and amplified 52 polymorphic loci. Primer Ax2 ⃰ had the highest discriminatory power. The population structure analysis classified the durum wheat collection into four populations. On average, population 4, consisting of 8 genotypes, had the highest allele number as well as genetic variation. Analysis of molecular variance indicated that 82% of the total variation was distributed among populations. The diversity among populations and gene flow were 0.14 and 3.03, respectively. The Jaccard distance coefficient revealed that genetic dissimilarities ranged from 0.031 between G62 and G65 to 0.725 between G36 and G51. Neighbor-joining method clustered individuals into six main groups. Results showed a remarkable level of genetic diversity among studied durum wheat genotypes which can be of interest for future breeding programs.

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“…Research, Society andDevelopment, v. 9, n. 12, e2991210648, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i12.10648 Os marcadores ISSR utilizados neste estudo mostraram-se bastante eficazes na determinação das variações genéticas entre os genótipos de trigo. Esses resultados estão de acordo com estudos anteriores obtidos por outros autores utilizando diferentes germoplasmas de trigo (Zeb et al, 2009;Mangini et al, 2010;Varsha et al, 2018;Nazarzadeh et al, 2020;Shaygan et al, 2020). Além disso, o presente estudo demonstrou que existe variabilidade genética no banco de germoplasma de trigo.…”

Section: Análise Estatística De Dados Issrsunclassified

“…Eles são particularmente interessantes uma vez que não necessitam do conhecimento prévio da sequência de DNA. Os ISSRs têm sido aplicados com sucesso em estudos sistemáticos de plantas, especialmente na identificação da variabilidade genética em germoplasma e na mensuração da variação para estabelecer relações evolutivas dentro ou entre espécies, subespécies ou populações e genomas (El-Assal & Gaber, 2012;Shaygan et al, 2020). Os marcadores ISSRs são encontradas em regiões genômicas de 100 a 3.000 pb, flanqueados por sequências de microssatélites, no qual são desenhados primers únicos e amplificados via PCR para gerar marcadores multilocos e dominates (Yang et al, 1996;Faleiro, 2007).…”

Section: Introductionunclassified

Marcadores ISSR são eficazes para acessar a diversidade genética em germoplasma de trigo (Triticum aestivum)

Polido

Bonacina

Ito

et al. 2020

RSD

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Conhecer a variabilidade genética existente entre cultivares de trigo (Triticum aestivum) é de grande importância para os programas de melhoramento. Desta forma, esse trabalho teve como objetivo utilizar marcadores ISSR para acessar a diversidade genética entre acessos contrastantes de trigo. Para isso, foram avaliados 32 acessos de trigo e um acesso de triticale provenientes do provenientes do Banco Ativo de Germoplasma de Trigo do Instituto de Desenvolvimento Rural do Paraná (IDR-Paraná). A partir de 10 primers de ISSR foram amplificados um total de 66 loci polimórficos com variação média de loci por primer de 6,6. A similaridade genética variou de 0,29 a 0,93, com média de 0,57. O dendrograma resultante mostrou 4 grupos, no qual observou-se que os genótipos LD 111202 e LD 111203 apresentaram alto índice de similaridade genética entre si. A análise bayesiana de agrupamento formou quatro grupos, parcialmente concordantes com o agrupamento hierárquico de UPGMA. Os marcadores ISSR foram capazes de discriminar acessos morfologicamente distintos e similares, além de fornecer informações consistentes sobre a variabilidade genética existente entre os genótipos de trigo que auxiliará nos programas de melhoramento genético.

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The study of genetic diversity in a minicore collection of durum wheat genotypes using agro-morphological traits and molecular markers

Cited by 14 publications

References 22 publications

Genetic Divergence in Micronutrient Rich wheat– A Tool to Identify Diverse Parents

Genetic Divergence in Micronutrient Rich wheat– A Tool to Identify Diverse Parents

Genetic Characterization of Rainfed Durum Wheat Genotypes Based On Functional Markers Associated With Grain Quality Proteins

Marcadores ISSR são eficazes para acessar a diversidade genética em germoplasma de trigo (Triticum aestivum)

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