The study of dielectrophoresis of cells using the optical technique of measuring

Fomchenkov, V. M.; Gavrilyuk, B. K.

doi:10.1007/bf02311219

Cited by 9 publications

(1 citation statement)

References 23 publications

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“…This method gave dielectrophoretic coefficients of the order of 10~25 A 2s4/kg for human red blood cells [5]. Another advantage of this method and of the method of rota tion [6,7] compared with other methods for investi gations of the effective dieletric properties of cells, e.g., by measuring the optical density of cell suspen sion [8] is that it opperates with single cells.…”

Section: Introductionmentioning

confidence: 97%

Protoplast Dielectrophoresis in Axisymmetric Fields

Stoicheva

Tsoneva

Dimitrov

1985

Zeitschrift Für Naturforschung C

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Pea protoplasts dielectrophoretic coefficients were measured in alternating electric fields of frequency 1 M Hz and voltage 5 V applied between two concentric cylindrical metal electrodes of outer and inner radii 0.24 mm and 1 mm, respectively. They do not vary significantly with solution osmolarity, but* show a clear expressed maximum in isotonic conditions; the values in 0.4 m , 0.5 m , 0.6 m and 0.7 m manitol solutions are (6.5, 10.2, 8.8 and 5.6) x lO * 24 A 2s4/kg, respectively. The average cell radii in those conditions are 14.2 (im, 14.6 [im, 13.8 jim and 13.6 [im. The radii of cell-to-electrode contacts follow the same dependence on the osmolarity as the dielectrophoretic coefficients; they do not depend on the applied voltages up to 18V. The times of cell approach near to the electrode were too short to be explained by the action only of the dielectrophoretic force; consequently an attractive force appeared at very close approach. These results may be of use in future studies of membrane adhesion and fusion.

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Section: Introductionmentioning

confidence: 97%

Protoplast Dielectrophoresis in Axisymmetric Fields

Stoicheva

Tsoneva

Dimitrov

1985

Zeitschrift Für Naturforschung C

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Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen

Krause

Schade

Gläser

et al. 1982

J of Basic Microbiology

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Dielectrophoresis as a new method for preparative separation of cells is being present5d. This method responds to differences in the polarization of cells, which correlate with a number of complex physiological properties of cells. It is shown t h a t yeast cells of different thermotolerant properties can be differentiated by dielectrophoresis. Moreover, the application of this method permitted to separate cells with a high selectivity, which produce more biomass a t a temperature of 40 "C than the initial population.Investigations on the ability of cells to survive in an inhomogeneous a. c. field have shown that the number of dead cells a t frequencies of lo5 cps and 106 cps and a t field strengths (on the surface of the central electrode) below 2.4 x lo5 V/m is small. This fact should be considered when picking out suitable separation conditions.

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Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen

Krause¹,

Schade²,

Gläser³

et al. 1982

Z Allg Mikrobiol

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Dielectrophoresis as a new method for preparative separation of cells is being presented. This method responds to differences in the polarization of cells, which correlate with a number of complex physiological properties of cells. It is shown that yeast cells of different thermotolerant properties can be differentiated by dielectrophoresis. Moreover, the application of this method permitted to separate cells with a high selectivity, which produce more biomass at a temperature of 40 degrees C than the initial population. Investigations on the ability of cells to survive in an inhomogeneous a.c. field have shown that the number of dead cells at frequencies of 10(5) cps and 10(6) cps and at field strengths (on the surface of the central electrode) below 2.4 X 10(5) V/m is small. This fact should be considered when picking out suitable separation conditions.

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The study of dielectrophoresis of cells using the optical technique of measuring

Cited by 9 publications

References 23 publications

Protoplast Dielectrophoresis in Axisymmetric Fields

Protoplast Dielectrophoresis in Axisymmetric Fields

Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen

Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen

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