The Study of Cyclic Diarylheptanoids, Flavonoids Isolation from Betula Hippolytii. Sukacz Leaves

Abstract

In Mongolia, there are 12 species of birch grow. Numerous researches conducted in Russia, Bulgaria, Japan, and China on B.pubescens, B.pendula, B.Rezniczenkoana (Litv) Schischk, B.humilis Schrank, and B.mandshurica Rgl Nakai found that birch barks and leaves contain antioxidants and they have anti-cancer, anti-yeast, anti-bacterial and anti-inflammatory properties, protect liver and promote bile secretion. In Mongolian traditional medicine, the birch leaves and barks are used to treat acute liver diseases. The birch leaves are considered to be not poisonous and detailed chemical compound structure of birch leaves hasn't been identified. Therefore, the purpose of this research is to conduct phytochemical research and isolate chemical compounds and their structures. The leaves of B.Hippolytii were powdered and extracted using 80% acetones. The fluid extracts were further evaporated and thickened. The thick-extract was suspended by ether, water and methanol 22.9 grams of diethyl ether were extracted and 12 compounds were identified using column and liquid chromatography methods and Mass spectral (MS) spectrometric methods including 13 C-NMR, 1 H-NMR, DEBT, COSY and HMBC methods. From the isolated 12 compounds, 4 compounds were cyclic diarylheptanoids and 8 compounds were flavonoids and their flavonol glycosides. The identified 4 diarylheptanoids compounds were macrocyclic diarylheptanoids: Alnusonol (4.4 mg), Alnusonon (5.6 mg), Acerogenin K (3.4 mg) and ether of diarylheptanoids 3,5'-dihydroxy-4'-methoxy-3',4''-oxo -1,7-diphenyl-1-heptene (6.5 mg). Flavonoids and their glycosides quercetin (1.6 mg), quercetin-3-O-(4-O-acetyl)-α-Lrhamnopyranoside (35.5 mg), myricetin (1.0mg), myricetin-3-O-rutinoside (1.0 mg), myricetin-3-O-(4-O-acetyl)-α-L-rhamnopyranoside (8.2 mg), keampferol-3-O-β-D-(6 // -O-E-p-coumaroyl) glucopyranoside (2.9 mg), kaempferol-3-O-α-L-rhamnopyranoside (11.9 mg) and acacetin-7-O-(4-O-acetyl)-α-L-rhamnopyranoside (9.9 mg). All compounds were isolated for the first time from this leaves B.Hippolytii.