The Study of Anticancer Activity of Satureja Khuzestanica Alcoholic Extracts On Expression of Bcl2 And Bax Genes In The Pc3 Cell Line

Abstract

Introduction: Prostate cancer is the most common cancer among men after lung cancer. It has grown in Iran in recent years. The use of medicinal plants is one of the most useful ways that causes the least side effects. Due to high levels of antioxidant compounds, Satureja khuzestanica is a good source for drug use to treat and prevent the development and progress of cancers. The aim of the present study was to evaluate the anti-cancer property of Satureja khuzestanica extract on the expression of Bcl2 and Bax genes in prostate cancer cell lines.Methodology: After collecting the plant in spring, the chloroform extract was prepared by rotary device. PC3 cancer cells were incubated at different concentrations of the extract for 24 hours. The inhibitory effect of the extract was evaluated using MTT assay as IC50. To evaluate apoptosis, the level of expression of Bax and BCL-2 genes after RNA extraction and transformation to cDNA were evaluated using Real Time PCR. All data were analyzed using REST software.Results: The results revealed a direct and significant relationship between the two variables of drug composition and rate of PC3 cell death. This composition increased Bax gene expression and decreased BCL-2 gene expression and induced apoptosis (P <0.05).Discussion and Conclusion: Based on the results, Satureja khuzestanica extract is likely to have anticancer properties and seems to be a new drug for killing prostate cancer cells.

The effects of methanolic extract of the aerial parts of Galium verum on HT29 and AGO cell lines

“…In general, active phytochemical components applied in ethnomedicine are mostly unknown, and the plant extract is considered an active ingredient [17]. The plant extract is a combination of active compounds, the combined action of which can mediate therapeutic activity.…”

“…Total RNA was extracted according to kit brochures provided by the manufacturer (cat: YT9080), and cDNA was synthesized (cat: YT4500). Gene expression was measured using the Amplicon Master Mix Kit under cycle conditions at 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 15 s with RT-PCR (Step One ABI), and gene analysis was performed using the Livak 2 −∆∆ct method [12,17]. The primer sequences are summarized in Table 1…”