The structure of Yersinia pestis Caf1 polymer in free and adjuvant bound states

Increasing the Potency of an Alhydrogel-Formulated Anthrax Vaccine by Minimizing Antigen-Adjuvant Interactions

“…Structural studies have shown that Alhydrogel-bound proteins, including rPA, preserve their secondary (19,20), tertiary (18), and quaternary (21,22) structures but exhibit decreased thermal stability, compared to their free counterparts in solution (23,24). At the surfaces of adjuvant particles, bound proteins form a monolayer in which individual biomolecules are packed closely together, with no apparent preference for any particular surface orientation.…”

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“…The highest values of AUROC among all injury severity objective methods were got for ВПХ-МТ (0,7569, (0,6448-0,869), р = 0,0001913) and for TRISS model (0,8076 (0,701-0,9142), р < 0,0001). Cut-off values were established according to Youden coefficient in points of ISS > 25,5 (acc = 65,75 %, odds = 5,044 (1,715-14,83), p = 0,0034), ВПХ-МТ > 13,15 (acc = 72,6 %, LR = 2,71, odds = 6,899 (2,43), p = 0,0003), RTS < 7,004 (acc = 78,08 %, odds = 12,57 (4,105-38,5), p < 0,0001) and TRISS < 0,8339 (acc = 79,45 %, LR = 5,69, odds = 15,54 (4,681-51,59), p < 0,0001). It is possible to calculate probability of mortality according to Bayes theorem with the help of this data considering that apriory probability is 18,47 -19,37 %.…”

“…Рисунок 2 показывает возможный механизм взаи-модействия полиадгезинов и системы секреции III типа (инъектисома). Фрагменты капсулы F1 Y. pestis слущиваются в культуральную среду [43], активиру-ют перитонеальные макрофаги in vitro и индуцируют продукцию провоспалительных цитокинов, TNF-α, IL-1 и IL-6, вдали от места инфекции, дезориенти-руя иммунную систему [42].…”

Identification of host factors required for Yersinia pestis macrophage intracellular survival and their impact on vacuole maturation, acidification and trafficking.

“…Lastly, Y. pestis has an atypical capsule encoded by the caf operon [1,[72][73][74]. This capsule is comprised mainly of helical proteins that are loosely attached to the outer membrane of the bacterium [1,[72][73][74].…”

“…This capsule is comprised mainly of helical proteins that are loosely attached to the outer membrane of the bacterium [1,[72][73][74]. Caf1 is an antiphagocytic virulence factor which prevents phagocytosis by macrophages and monocytes [75,76].…”