1999
DOI: 10.1016/s0092-8674(00)80963-0
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The Structure of the Protein Phosphatase 2A PR65/A Subunit Reveals the Conformation of Its 15 Tandemly Repeated HEAT Motifs

Abstract: The PR65/A subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit, generating functionally diverse heterotrimers. Mutations of the beta isoform of PR65 are associated with lung and colon tumors. The crystal structure of the PR65/Aalpha subunit, at 2.3 A resolution, reveals the conformation of its 15 tandemly repeated HEAT sequences, degenerate motifs of approximately 39 amino acids present in a variety of protei… Show more

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Cited by 406 publications
(357 citation statements)
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References 60 publications
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“…Domain searches also predicted the presence of five HEAT (huntingtin-elongation-A subunit-TOR) motifs (Groves et al, 1999) distributed throughout KapI as indicated in Fig. 1(a) (dark rectangles).…”
Section: Resultsmentioning
confidence: 98%
“…Domain searches also predicted the presence of five HEAT (huntingtin-elongation-A subunit-TOR) motifs (Groves et al, 1999) distributed throughout KapI as indicated in Fig. 1(a) (dark rectangles).…”
Section: Resultsmentioning
confidence: 98%
“…Recent structural studies of PI3K␥ show that it contains many modular regions including a helical domain (also called a HEAT sequence motif) (14) that could be involved in proteinprotein interactions (24,25). Since the HEAT sequence is common to PI3K␣, -␤, and -␥ isoforms, it is possible that this is the common interacting domain for all PI3Ks.…”
Section: Discussionmentioning
confidence: 99%
“…Residues 1-321 are organized in classical HEAT repeats 13 with a total of 16 helices. Instead of forming a single long, curved structure as found in PP2A 14 , the HEAT domain of eEF3 makes a sharp bend after the first 10 helices. The HEAT domain is connected through a flexible linker to a four-helix bundle (4HB domain; residues 336-413), which packs against the bottom of the concave face of the HEAT domain.…”
Section: Crystal Structure Of Eef3mentioning
confidence: 94%