The structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas bestiarum strain 207

Turska-Szewczuk, Anna; Kozinska, A.; Russa, Ryszard; Holst, Otto

doi:10.1016/j.carres.2009.12.030

Cited by 21 publications

(17 citation statements)

References 25 publications

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“…A prominent member of this genus found in this study, Aeromonas hydrophila , can cause a variety of diseases in humans from gastrointestinal disorders to more severe illnesses such as septicemia, meningitis, wound infections, etc. [45, 46]. Bacillus spp.…”

Section: Resultsmentioning

confidence: 99%

Microbial Diversity of Source and Point-of-Use Water in Rural Haiti – A Pyrosequencing-Based Metagenomic Survey

et al. 2016

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Haiti endures the poorest water and sanitation infrastructure in the Western Hemisphere, where waterborne diseases cause significant morbidity and mortality. Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of selected source and point-of-use water from rural areas in Central Plateau, Haiti using pyrosequencing of 16s rRNA genes. Taxonomic composition of water samples revealed an abundance of Firmicutes phyla, followed by Proteobacteria and Bacteroidetes. A total of 38 bacterial families and 60 genera were identified. The presence of several Klebsiella spp. (tentatively, K. pneumoniae, K. variicola and other Klebsiella spp.) was detected in most water samples. Several other human pathogens such as Aeromonas, Bacillus, Clostridium, and Yersinia constituted significantly higher proportion of bacterial communities in the point-of-use water samples compared to source water. Bacterial genera traditionally associated with biofilm formation, such as Chryseobacterium, Fusobacterium, Prevotella, Pseudomonas were found in the point-of-use waters obtained from water filters or domestic water storage containers. Although the pyrosequencing method utilized in this study did not reveal the viability status of these pathogens, the abundance of genetic footprints of the pathogens in water samples indicate the probable risk of bacterial transmission to humans. Therefore, the importance of appropriate handling, purification, and treatment of the source water needed to be clearly communicated to the communities in rural Haiti to ensure the water is safe for their daily use and intake.

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Section: Resultsmentioning

confidence: 99%

Microbial Diversity of Source and Point-of-Use Water in Rural Haiti – A Pyrosequencing-Based Metagenomic Survey

et al. 2016

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“…20 In the TOCSY spectrum cross-peaks between H-1 and H-2; H-2 and H-3,4,5,6 as well as correlations between H-6 and H-2-H-5 were observed for the Rha spin system. 25 The large 3 J 2,3 , 3 J 3,4 , and 3 J 4,5 coupling constant values ($10 Hz), 22 and correlations of each H-1, to H-6 with all other protons of residue B in the TOCSY spectrum provided evidence for the gluco configuration of ring system B. In addition, in the NOESY spectrum correlations between the H-1,H-3 and H-1,H-5 pairs of protons confirmed the b-anomeric configuration of B.…”

Section: Resultsmentioning

confidence: 97%

“…A majority of the hitherto described repeating units of OPS from Aeromonas species bacteria contained 6-deoxyhexopyranoses Rhap 15,25,[28][29][30] or 6dTalp 14,16 as constituents. The disaccharidic structure of the OPS from A. sobria strain Pt312 presented in this paper is similar to that of the OPS from A. hydrophila strain LL1 in both the sugar composition and glycosylation pattern.…”

Section: Resultsmentioning

confidence: 99%

“…23,24 The latter conclusion was further confirmed by the intra-residue H-1,H-2 correlations observed in the NOESY spectrum (as compared to published data). 19,[24][25][26] The manno configuration of A was inferred on the basis of relatively high coupling constant values of 3 J 3,4 and 3 J 4,5 equal to $10 Hz contrasting with the small value of 3 J 2,3 $3.5 Hz. 20 In the TOCSY spectrum cross-peaks between H-1 and H-2; H-2 and H-3,4,5,6 as well as correlations between H-6 and H-2-H-5 were observed for the Rha spin system.…”

Section: Resultsmentioning

confidence: 99%

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Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312

Turska-Szewczuk

Pietras

Duda

et al. 2015

Carbohydrate Research

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“…The sequence of the sugar residues was determined by ROESY and HMBC experiments. It is concluded that the O -polysaccharide is composed of branched pentasaccharide repeating units (31) [121]:…”

Section: Structure Of Carbohydrate Antigens Of Gammaproteobacteriamentioning

confidence: 99%

The Structural Diversity of Carbohydrate Antigens of Selected Gram-Negative Marine Bacteria

2011

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Marine microorganisms have evolved for millions of years to survive in the environments characterized by one or more extreme physical or chemical parameters, e.g., high pressure, low temperature or high salinity. Marine bacteria have the ability to produce a range of biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents, and as a result, they have been a topic of research interest for many years. Among these biologically active molecules, the carbohydrate antigens, lipopolysaccharides (LPSs, O-antigens) found in cell walls of Gram-negative marine bacteria, show great potential as candidates in the development of drugs to prevent septic shock due to their low virulence. The structural diversity of LPSs is thought to be a reflection of the ability for these bacteria to adapt to an array of habitats, protecting the cell from being compromised by exposure to harsh environmental stress factors. Over the last few years, the variety of structures of core oligosaccharides and O-specific polysaccharides from LPSs of marine microrganisms has been discovered. In this review, we discuss the most recently encountered structures that have been identified from bacteria belonging to the genera Aeromonas, Alteromonas, Idiomarina, Microbulbifer, Pseudoalteromonas, Plesiomonas and Shewanella of the Gammaproteobacteria phylum; Sulfitobacter and Loktanella of the Alphaproteobactera phylum and to the genera Arenibacter, Cellulophaga, Chryseobacterium, Flavobacterium, Flexibacter of the Cytophaga-Flavobacterium-Bacteroides phylum. Particular attention is paid to the particular chemical features of the LPSs, such as the monosaccharide type, non-sugar substituents and phosphate groups, together with some of the typifying traits of LPSs obtained from marine bacteria. A possible correlation is then made between such features and the environmental adaptations undertaken by marine bacteria.

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The structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas bestiarum strain 207

Cited by 21 publications

References 25 publications

Microbial Diversity of Source and Point-of-Use Water in Rural Haiti – A Pyrosequencing-Based Metagenomic Survey

Microbial Diversity of Source and Point-of-Use Water in Rural Haiti – A Pyrosequencing-Based Metagenomic Survey

Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312

The Structural Diversity of Carbohydrate Antigens of Selected Gram-Negative Marine Bacteria

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