The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species

Kowalczyk, Agnieszka; Szpakowska, Nikola; Śledź, Wojciech; Motyka-Pomagruk, Agata; Ossowska, Karolina; Łojkowska, Ewa; Kaczyński, Zbigniew

doi:10.1016/j.carres.2020.108135

Cited by 7 publications

(13 citation statements)

References 13 publications

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“…The OPS of P. aquaticum IFB5637 LPS turned out to contain three different monosaccharides, namely, mannose, glucose, and abequose (3,6-dideoxy-D-xylo-hexose). Mannose and glucose are relatively common monosaccharides in bacterial O-polysaccharides, while abequose has not been identified yet in any strains of the Pectobacteriaceae family [ 59 , 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

“…Isolation and determination of the sugar composition of the O-polysaccharide (OPS) of bacterial lipopolysaccharide (LPS) was performed as described by Kowalczyk et al [ 59 ]. The analyzed strain was plated from frozen stock and cultured on TSA medium at 28 °C for 24 h. Then, 200 mL TSB medium was inoculated with a single bacterial colony taken from the TSA plate and subsequently incubated at 28 °C with 130 rpm shaking for 24 h. This overnight culture was used for inoculation of 2 L of TSB medium.…”

Section: Methodsmentioning

confidence: 99%

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The First Polish Isolate of a Novel Species Pectobacterium aquaticum Originates from a Pomeranian Lake

Babinska

Motyka-Pomagruk

Śledź

et al. 2021

IJERPH

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Pectinolytic bacteria from the genus Pectobacterium cause high economic losses in various crops, vegetables, and ornamentals including potato. Thus far, these strains have been isolated from distinct environments such as rotten or asymptomatic plants, soil, and waterways. The prevalence of soft rot Pectobacteriaceae in different depths of Pomeranian lakes was performed by a qualified scuba diver over 2 years of monitoring. It allowed for the isolation and broad characterization of a strain from the newly established species Pectobacterium aquaticum. Phylogenetic analysis on the sequences of dnaX and recA genes revealed the highest similarity of this strain to P. aquaticum CFBP 8637T. In addition to the determination of analytical profile index (API 20E), we discovered that this strain possesses a smooth form of a lipopolysaccharide with O-polysaccharide consisting of mannose, glucose, and abequose. Moreover, the characterized strain, described as P. aquaticum IFB5637, produced plant-cell–wall-degrading enzymes, such as pectinases, cellulases, proteases, and was capable of macerating potato and chicory tissues under laboratory conditions. In view of more frequent irrigation of seed potato fields resulting from the ongoing climate warming, it is important to monitor the occurrence of potential disease-causing agents in natural waterways.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The First Polish Isolate of a Novel Species Pectobacterium aquaticum Originates from a Pomeranian Lake

Babinska

Motyka-Pomagruk

Śledź

et al. 2021

IJERPH

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“…Three other OPS from pectinolytic bacteria from the closely related genus Dickeya were also structurally identified. In more detail, an identical OP structure was detected in four strains of D. solani and a D. dadantii 3937 strain [25,26], whil a different molecule was noted in D. aquatica IFB0154 [27]. All things considered, ther To date, only several structures of O-polysaccharides of the LPS of Pectobacterium spp.…”

Section: The Chemical Structure Of Ops Of P Parmentieri Scc3193 and I...mentioning

confidence: 98%

“…For instance, D. aquatica strain IFB0154 isolated from water had a different OPS structure (which contained fucose and rhamnose) than that of the other investigated Dickeya spp. strains isolated from diseased plants (in their case the OPS consisted of 6-deoxyaltrose repeating units) [25,27]. Notably, D. aquatica IFB0154 was reported to be much less efficient in rotting potato and chicory tissues than the other members of the Dickeya genus [28].…”

Section: Analysis Of the Phenotypic Features And Ability To Cause Dis...mentioning

confidence: 99%

Heterogenicity within the LPS Structure in Relation to the Chosen Genomic and Physiological Features of the Plant Pathogen Pectobacterium parmentieri

Ossowska

Motyka-Pomagruk

Kaczyńska

et al. 2022

IJMS

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Pectobacterium parmentieri is a pectinolytic plant pathogenic bacterium causing high economic losses of cultivated plants. The highly devastating potential of this phytopathogen results from the efficient production of plant cell wall-degrading enzymes, i.e., pectinases, cellulases and proteases, in addition to the impact of accessory virulence factors such as motility, siderophores, biofilm and lipopolysaccharide (LPS). LPS belongs to pathogen-associated molecular patterns (PAMPs) and plays an important role in plant colonization and interaction with the defense systems of the host. Therefore, we decided to investigate the heterogeneity of O-polysaccharides (OPS) of LPS of different strains of P. parmentieri, in search of an association between the selected genomic and phenotypic features of the strains that share an identical structure of the OPS molecule. In the current study, OPS were isolated from the LPS of two P. parmentieri strains obtained either in Finland in the 1980s (SCC3193) or in Poland in 2013 (IFB5432). The purified polysaccharides were analyzed by utilizing 1D and 2D NMR spectroscopy (1H, DQF-COSY, TOCSY, ROESY, HSQC, HSQC-TOCSY and HMBC) in addition to chemical methods. Sugar and methylation analyses of native polysaccharides, absolute configuration assignment of constituent monosaccharides and NMR spectroscopy data revealed that these two P. parmentieri strains isolated in different countries possess the same structure of OPS with a very rare residue of 5,7-diamino-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (pseudaminic acid) substituted in the position C-8: →3)-β-d-Galf-(1→3)-α-d-Galp-(1→8)-β-Pse4Ac5Ac7Ac-(2→6)-α-d-Glcp-(1→6)-β-d-Glcp-(1→. The previous study indicated that three other P. parmentieri strains, namely IFB5427, IFB5408 and IFB5443, exhibit a different OPS molecule than SCC3193 and IFB5432. The conducted biodiversity-oriented assays revealed that the P. parmentieri IFB5427 and IFB5408 strains possessing the same OPS structure yielded the highest genome-wide similarity, according to average nucleotide identity analyses, in addition to the greatest ability to macerate chicory tissue among the studied P. parmentieri strains. The current research demonstrated a novel OPS structure, characteristic of at least two P. parmentieri strains (SCC3193 and IFB5432), and discussed the observed heterogenicity in the OPS of P. parmentieri in a broad genomic and phenotype-related context.

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“…[ 97 , 98 , 99 , 100 , 101 , 102 ] and Dickeya sp. [ 80 , 95 , 103 , 104 ] have been revealed by NMR, and they were found to be very diverse. It is reasonable to propose that additional tailspikes should provide recognition of OPS with different structures.…”

Section: Morphotype Myoviridaementioning

confidence: 99%

Tailed Lytic Bacteriophages of Soft Rot Pectobacteriaceae

et al. 2021

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The study of the ecological and evolutionary traits of Soft Rot Pectobacteriaceae (SRP) comprising genera Pectobacterium and Dickeya often involves bacterial viruses (bacteriophages). Bacteriophages are considered to be a prospective tool for the ecologically safe and highly specific protection of plants and harvests from bacterial diseases. Information concerning bacteriophages has been growing rapidly in recent years, and this has included new genomics-based principles of taxonomic distribution. In this review, we summarise the data on phages infecting Pectobacterium and Dickeya that are available in publications and genomic databases. The analysis highlights not only major genomic properties that assign phages to taxonomic families and genera, but also the features that make them potentially suitable for phage control applications. Specifically, there is a discussion of the molecular mechanisms of receptor recognition by the phages and problems concerning the evolution of phage-resistant mutants.

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The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species

Cited by 7 publications

References 13 publications

The First Polish Isolate of a Novel Species Pectobacterium aquaticum Originates from a Pomeranian Lake

The First Polish Isolate of a Novel Species Pectobacterium aquaticum Originates from a Pomeranian Lake

Heterogenicity within the LPS Structure in Relation to the Chosen Genomic and Physiological Features of the Plant Pathogen Pectobacterium parmentieri

Tailed Lytic Bacteriophages of Soft Rot Pectobacteriaceae

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