The Structure of the Cyclopropylmethyl Carbonium Ion

Richey, Herman G.; Richey, Jane M.

doi:10.1021/ja00973a035

Cited by 22 publications

(4 citation statements)

References 3 publications

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“…Anion-exchange chromatography separates substances according to their net negative charge. The Mono Q column we have used for this purpose provides separations similar to those achieved with DEAEcellulose, using the same buffer systems (Richey, 1982). We have been able to achieve some satisfactory resolution of the smaller E2-and F-components ( Fig.…”

Section: Discussionmentioning

confidence: 76%

Chromatographic resolution of chicken phosvitin. Multiple macromolecular species in a classic vitellogenin-derived phosphoprotein

Wallace¹

1986

Biochemical Journal

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Chicken phosvitin was prepared from egg yolk by a variety of published methods, including a modification of our own original procedure. Yolk granules and all phosvitin preparations have been previously found to contain major phosphoproteins at Mr 40,000 and 33,000 and minor satellite components when electrophoresed on polyacrylamide gradient gels and stained with Stains-all. However, only our current preparation contained three additional phosphoproteins (Mr 18,000, 15,000 and 13,000) that are also present in yolk granules. Our current phosvitin preparation also appeared to have additional components when compared with other preparations by size-exclusion and anion-exchange chromatography. Particularly complex but entirely reproducible patterns were obtained by hydrophobic-interaction chromatography. However, a cross-referencing of fractions eluted by size-exclusion chromatography to the other procedures employed, including gel electrophoresis, reinforced the notion that unfractionated chicken phosvitin contains at least five major components, designated B, C, E1, E2 and F for the Mr 40,000, 33,000, 15,000, 18,000, and 13,000 phosphoproteins, respectively. Stoichiometric considerations lead us to suggest that vitellogenin I gives rise to phosvitins C and F, vitellogenin II gives rise to phosvitin B, and vitellogenin III gives rise to either phosvitin E1 or E2, but not both. Thus, a fourth, as yet undetected, vitellogenin may exist for the chicken.

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Section: Discussionmentioning

confidence: 76%

Chromatographic resolution of chicken phosvitin. Multiple macromolecular species in a classic vitellogenin-derived phosphoprotein

Wallace¹

1986

Biochemical Journal

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“…Iridectomies had skeptics since they were not uniformly effective, although iridectomies did prevent blindness in some individuals and the mechanism of action (ie, prevention of pupillary block) was not understood. 36 The case is also noteworthy because it documents Cushing's process for using a relatively new treatment strategy for a disorder with which he had relatively little prior experience. Cushing did not perform the treatment without first consulting Mills, an instructor of ophthalmology at Johns Hopkins who clearly expressed approval.…”

Section: Commentmentioning

confidence: 95%

Insight Into Glaucoma Treatment in the Early 1900s

et al. 2012

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“…This alcohol had vpc and spectral characteristics completely congruent with those of peak 2 of the deamination. Nmr (CC1«) peaks appeared at 0.10-0.55 ppm (multiplet, 4 ), 0.58-0.90 (multiplet, 1 ), 0.91 (center of CH3 doublet, 6 ), 1.63 (center of symmetrical quintet), 2.0 (OH), 2.48 (center of X quartet, J = 7 cps, 1 H). Anal.…”

Section: Methodsmentioning

confidence: 99%