The structure of the capsular polysaccharide from a swarming strain of pathogenic Proteus vulgaris

Rahman, Motiur; Guard, Jean; Asokan, K.; Carlson, Russell W.

doi:10.1016/s0008-6215(97)00093-1

Cited by 16 publications

(3 citation statements)

References 23 publications

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“…Capsule preparations were separated by deoxycholic acid (MP Biomedicals) polyacrylamide gel electrophoresis (DOC-PAGE) [65] and visualised by silver stain (Bio-Rad).…”

Section: Methodsmentioning

confidence: 99%

Attachment and Invasion of Neisseria meningitidis to Host Cells Is Related to Surface Hydrophobicity, Bacterial Cell Size and Capsule

et al. 2013

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We compared exemplar strains from two hypervirulent clonal complexes, strain NMB-CDC from ST-8/11 cc and strain MC58 from ST-32/269 cc, in host cell attachment and invasion. Strain NMB-CDC attached to and invaded host cells at a significantly greater frequency than strain MC58. Type IV pili retained the primary role for initial attachment to host cells for both isolates regardless of pilin class and glycosylation pattern. In strain MC58, the serogroup B capsule was the major inhibitory determinant affecting both bacterial attachment to and invasion of host cells. Removal of terminal sialylation of lipooligosaccharide (LOS) in the presence of capsule did not influence rates of attachment or invasion for strain MC58. However, removal of either serogroup B capsule or LOS sialylation in strain NMB-CDC increased bacterial attachment to host cells to the same extent. Although the level of inhibition of attachment by capsule was different between these strains, the regulation of the capsule synthesis locus by the two-component response regulator MisR, and the level of surface capsule determined by flow cytometry were not significantly different. However, the diplococci of strain NMB-CDC were shown to have a 1.89-fold greater surface area than strain MC58 by flow cytometry. It was proposed that the increase in surface area without changing the amount of anchored glycolipid capsule in the outer membrane would result in a sparser capsule and increase surface hydrophobicity. Strain NMB-CDC was shown to be more hydrophobic than strain MC58 using hydrophobicity interaction chromatography and microbial adhesion-to-solvents assays. In conclusion, improved levels of adherence of strain NMB-CDC to cell lines was associated with increased bacterial cell surface and surface hydrophobicity. This study shows that there is diversity in bacterial cell surface area and surface hydrophobicity within N. meningitidis which influence steps in meningococcal pathogenesis.

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“…Capsule preparations were separated by deoxycholic acid (MP Biomedicals) polyacrylamide gel electrophoresis (DOC-PAGE) [65] and visualised by silver stain (Bio-Rad).…”

Section: Methodsmentioning

confidence: 99%

Attachment and Invasion of Neisseria meningitidis to Host Cells Is Related to Surface Hydrophobicity, Bacterial Cell Size and Capsule

et al. 2013

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“…To avoid in vivo immune clearance, [21] we extracted CPs from S. pneumoniae, a bacterium known for its ability to colonize the lung, using a thermal enzymatic hydrolysis method. [22] The successful extraction of CPs was confirmed by an obvious absorption peak at 206 nm in the UV-vis spectrum (Figure 3b and Figure S8c, Supporting Information). The bioinspired GaTa-CP NPs were constructed by shielding GaTa network with CP cloaks.…”

Section: Construction and Characterization Of Bioinspired Gata-cp@eto...mentioning

confidence: 86%

Inhalable Capsular Polysaccharide‐Camouflaged Gallium‐Polyphenol Nanoparticles Enhance Lung Cancer Chemotherapy by Depleting Local Lung Microbiota

et al. 2023

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Local lung microbiota is closely associated with lung tumorigenesis and therapeutic response. It is found that lung commensal microbes induce chemoresistance in lung cancer by directly inactivating therapeutic drugs via biotransformation. Accordingly, an inhalable microbial capsular polysaccharide (CP)‐camouflaged gallium‐polyphenol metal–organic network (MON) is designed to eliminate lung microbiota and thereby abrogate microbe‐induced chemoresistance. As a substitute for iron uptake, Ga3+ released from MON acts as a “Trojan horse” to disrupt bacterial iron respiration, effectively inactivating multiple microbes. Moreover, CP cloaks endow MON with reduced immune clearance by masquerading as normal host‐tissue molecules, significantly increasing residence time in lung tissue for enhanced antimicrobial efficacy. In multiple lung cancer mice models, microbe‐induced drug degradation is remarkably inhibited when drugs are delivered by antimicrobial MON. Tumor growth is sufficiently suppressed and mouse survival is prolonged. The work develops a novel microbiota‐depleted nanostrategy to overcome chemoresistance in lung cancer by inhibiting local microbial inactivation of therapeutic drugs.

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“…Alternatively, the tail spike might be active against a capsular polysaccharide (CPS) produced by Proteus cells. A number of publications report the presence of a capsule and its involvement in the formation of crystalline biofilms by Proteus mirabilis [ 4 , 79 , 80 , 81 , 82 , 83 , 84 ], although not all strains of Proteus can synthesize it [ 85 ]; no capsule was detected following multiple capsule staining attempts of P. mirabilis BB2000 (data not shown). In Proteus strains that produce CPS, its structure appears to be identical to O-specific chains of their LPS [ 79 , 85 , 86 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis Biofilms

et al. 2021

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The adherence of Proteus mirabilis to the surface of urinary catheters leads to colonization and eventual blockage of the catheter lumen by unique crystalline biofilms produced by these opportunistic pathogens, making P. mirabilis one of the leading causes of catheter-associated urinary tract infections. The Proteus biofilms reduce efficiency of antibiotic-based treatment, which in turn increases the risk of antibiotic resistance development. Bacteriophages and their enzymes have recently become investigated as alternative treatment options. In this study, a novel Proteus bacteriophage (vB_PmiS_PM-CJR) was isolated from an environmental sample and fully characterized. The phage displayed depolymerase activity and the subsequent genome analysis revealed the presence of a pectate lyase domain in its tail spike protein. The protein was heterologously expressed and purified; the ability of the purified tail spike to degrade Proteus biofilms was tested. We showed that the application of the tail spike protein was able to reduce the adherence of bacterial biofilm to plastic pegs in a MBEC (minimum biofilm eradication concentration) assay and improve the survival of Galleria mellonella larvae infected with Proteus mirabilis. Our study is the first to successfully isolate and characterize a biofilm depolymerase from a Proteus phage, demonstrating the potential of this group of enzymes in treatment of Proteus infections.

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The structure of the capsular polysaccharide from a swarming strain of pathogenic Proteus vulgaris

Cited by 16 publications

References 23 publications

Attachment and Invasion of Neisseria meningitidis to Host Cells Is Related to Surface Hydrophobicity, Bacterial Cell Size and Capsule

Attachment and Invasion of Neisseria meningitidis to Host Cells Is Related to Surface Hydrophobicity, Bacterial Cell Size and Capsule

Inhalable Capsular Polysaccharide‐Camouflaged Gallium‐Polyphenol Nanoparticles Enhance Lung Cancer Chemotherapy by Depleting Local Lung Microbiota

Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis Biofilms

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