The structure of O-polysaccharides isolated from plant pathogenic bacteria Pectobacterium wasabiae IFB5408 and IFB5427

Ossowska, Karolina; Czerwicka, Małgorzata; Śledź, Wojciech; Żołędowska, Sabina; Motyka, Agata; Szulta, Sylwia; Łojkowska, Ewa; Kaczyński, Zbigniew

doi:10.1016/j.carres.2016.03.017

Cited by 17 publications

(18 citation statements)

References 19 publications

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“…Despite the diversity of species and strains comprising SRP, very limited information is available on both the composition of polysaccharides of Pectobacterium and Dickeya, and the details of phage interaction with the polysaccharides. Only eight structures of polysaccharides of P. atrosepticum [36,46,47], P. wasabiae [48], P. carotovorum (then Erwinia carotovora subsp. carotovora) [49], P. brasilense [45] and D. solani [44,50] have been identified.…”

Section: Discussionmentioning

confidence: 99%

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Shneider

Lukianova

Evseev

et al. 2020

IJMS

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Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.

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Section: Discussionmentioning

confidence: 99%

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Shneider

Lukianova

Evseev

et al. 2020

IJMS

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“…Attachment of the tail spike to the OPS on the bacterial surface and enzymatic depolymerization of the polysaccharide are key initial events in phage infection. The structure of the OPS is the same for all D. solani strains (Ossowska et al, 2016), as well as some other Pectobacteriaceae and Enterobacteriaceae . We present an example of Enterobacteriaceae phage host Lelliottia F154.…”

Section: Discussionmentioning

confidence: 99%

Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host

et al. 2019

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Dickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriophages recently re-ranked as a separate Ackermannviridae family, such as phage PP35 described in this work, are the attractive candidates for this bacterial biocontrol. PP35 has a very specific host range due to the presence of tail spike protein PP35 gp156, which can depolymerize the O-polysaccharide (OPS) of D. solani. The D. solani OPS structure, →2)-β-D-6-deoxy-D-altrose-(1→, is so far unique among soft-rot Pectobacteriaceae, though it may exist in non-virulent environmental Enterobacteriaceae. The phage tail spike depolymerase degrades the shielding polysaccharide, and launches the cell infection process. We hypothesize that non-pathogenic commensal bacteria may maintain the population of the phage in soil environment.

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“…Methylation analysis was performed to determine the substitution position of sugar residues in OPS. It was made by the Ciukanu and Kerek method using methyl iodine in DMSO in the presence of potassium hydroxide [16,17]. The methylated OPS was hydrolyzed, reduced and acetylated, as described above.…”

Section: Compositional Analysesmentioning

confidence: 99%

“…The LPS (335 mg) was isolation from dried bacterial cells using Westphal and Jann methods then purified by nucleic acid precipitation with 40% ethanol at pH 4.5. Finally, LPS was precipitated with 80% ethanol at pH 7.0 [15,16]. The LPS (150 mg) was hydrolyzed with 1% acetic acid at 100 C for 2 h. Lipid A was removed from the mixture by centrifugation.…”

Section: Bacterial Growth Lps and Ops Isolationmentioning

confidence: 99%

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Structural characterization of the O-polysaccharide isolated from Franconibacter helveticus LMG23732T

Szulta

Czerwicka

Forsythe

et al. 2016

Carbohydrate Research

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The structure of O-polysaccharides isolated from plant pathogenic bacteria Pectobacterium wasabiae IFB5408 and IFB5427

Cited by 17 publications

References 19 publications

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host

Structural characterization of the O-polysaccharide isolated from Franconibacter helveticus LMG23732T

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