The structure of mytilan, a bioglycan-immunomodulator isolated from the mussel Crenomytilus grayanus

Оводова, Р. Г.; Glazkova, Vera E.; Mikheyskaya, Lidiya V.; Molchanova, V. I.; Исаков, В. В.; Ovodov, Yu. S.; Molina, Lucas Matías

doi:10.1016/0008-6215(92)80018-v

Cited by 25 publications

(9 citation statements)

References 6 publications

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“…Mollusks are an excellent source of high‐quality nutrition in China as well as other parts of the world and have been used as food products and traditional Chinese medicinal drugs for centuries. In recent years, the water‐soluble polysaccharides present in mollusks have become the focus of intense interest due to their various bioactivities, such as immunomodulation activity (Dai, Zhang, Zhang, & Wang, ; Ovodova et al., ), antioxidant, and hepato‐protective activities (Jiang, Wang, Liu, Gan, & Zeng, ; Liao et al., ).…”

Section: Introductionmentioning

confidence: 99%

Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis

Qin

Fan

Zhang

et al. 2018

Food Science & Nutrition

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Crude polysaccharide was prepared from Crassostrea rivularis by 30% (w/v) potassium hydroxide solution at 90°C for 120 min. Three fractions (OG1, OG2, and OG3) were purified by DEAE‐52 cellulose and Sepharose 2B gel column chromatography. The chemical structures were determined using gas chromatography (GC), high‐performance gel permeation chromatography (HPGPC), Fourier‐transform infrared (FT‐IR) spectroscopy, and 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. The results indicated that OG1 was composed of rhamnose and little mannose (8.71%), the ratio of Rha: Gal: Xyl: Fuc in OG3 were 14:5.5:3:1. And their average molecular weights (Mw) were about 1.66 × 106 and 2.33 × 106 Da, respectively. OG2 was composed only of glucose (98.23%), which means it was glycogen. OG2 was consisted mainly of →4)‐ α‐D‐Glc‐(1→, with the branch chain every 6.5 glucose residues on average, which is →4,6)‐α‐D‐Glc‐(1→ and trace amount of α‐D‐Glc‐(1→ branched units. The Mw was 2.27 × 106 Da. It provides the bases for the bioactivity research.

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Section: Introductionmentioning

confidence: 99%

Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis

Qin

Fan

Zhang

et al. 2018

Food Science & Nutrition

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“…Some studies have investigated the structure and bioactivity of compounds from other shellfishes . Mytilan, isolated from the mantle of the mussel Crenomytilus grayanus , was shown to demonstrate high immunomodulating activity . A polysaccharide (glycogen) generated from Perna canaliculus was found to exhibit antiinflammatory activity .…”

Section: Introductionmentioning

confidence: 99%

“…3 Mytilan, isolated from the mantle of the mussel Crenomytilus grayanus, was shown to demonstrate high immunomodulating activity. 4 A polysaccharide (glycogen) generated from Perna canaliculus was found to exhibit antiinflammatory activity. 5 A heparin-like substance separated from the marine clam Anomalocardia brasiliana was able to bind antithrombin III, and was demonstrated to have effective anticoagulant activity.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry analysis and in silico prediction of allergenicity of peptides in tryptic hydrolysates of the proteins from Ruditapes philippinarum

Liu

et al. 2017

J Sci Food Agric

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“…If valid, the finding by Arvindekar and Patil 3 may add new clues to the elucidation of the bioactive components of the S. cerevisiae cell wall 33 . The bioactivity of (1→4)-α-D-glucans from microbial 10 , fungal 15 , plant 8,32 or animal 27 origin is well documented. Therefore, any additional research data that can confirm or deny the presence of (1→4)-α-D-glucan in a covalently bound form in S. cerevisiae cell wall might have, not only scientific, but also commercial value 29 .…”

Section: Introductionmentioning

confidence: 99%

A Study of Saccharomyces cerevisiae Cell Wall Glucans

Kwiatkowski

Thielen

Glenney

et al. 2009

Journal of the Institute of Brewing

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Much research has been carried out over the years examining cell wall glucans from Saccharomyces cerevisiae and this study further examines aspects of the binding of (1r̊4)‐α‐D‐glucan in the yeast cell wall, using a number of isolation techniques as well as monoclonal antibodies able to recognize a mixed (1r̊4)‐α‐D‐glucan/(1r̊6)‐β‐D‐glucan. Extraction of purified glucan, from S. cerevisiae cell wall, with 0.1N HCl, at 80°C for 6 h, released into the solution (1r̊4)‐α‐D‐glucan and (1r̊6)‐β‐D‐glucan as the major polysaccharides, along with an insoluble pellet highly enriched in (1r̊3)‐β‐D‐glucan. The released (1r̊4)‐α‐D‐glucan was composed of a high molecular size >100 kDa fraction (7.2% w/w) and a medium 5–50 kDa polysaccharide (10.2% w/w), with the (1r̊4)‐α‐D‐glucan covalently bound to the (1r̊6)‐β‐D‐glucan. The average molar ratio of the α:β glucan was 47: 53 in this mixed polysaccharide. The structure of this polysaccharide was different from the structure of plant starch or animal glycogen as monoclonal antibodies specific to yeast (1r̊4)‐α‐D‐glucan/(1r̊6)‐β‐D‐glucan did not recognize the plant starch or animal glycogen standards.

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The structure of mytilan, a bioglycan-immunomodulator isolated from the mussel Crenomytilus grayanus

Cited by 25 publications

References 6 publications

Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis

Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis

Mass spectrometry analysis and in silico prediction of allergenicity of peptides in tryptic hydrolysates of the proteins from Ruditapes philippinarum

A Study of Saccharomyces cerevisiae Cell Wall Glucans

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