The Structure of Human High Density Lipoprotein and the Levels of Apolipoprotein A-I in Plasma as Determined by Radioimmunoassay

Schonfeld, Gustav; Pfleger, Barbara

doi:10.1172/jci107758

Cited by 209 publications

(43 citation statements)

References 36 publications

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“…Provided that similar methods are used for the pretreatment of the sera, our data are in good agreement with data from other authors (4)(5)(6)(7)(8). Both methods, RIA and EID, produce comparable results, but reproducibility seems to be slightly higher with the EID.…”

Section: Discussionsupporting

confidence: 90%

“…Several assays for the quantitative determination of apolipoproteins A-I and A-II, the two major apolipoproteins of HDL, have been published during the last few years (4)(5)(6)(7)(8)(9). The objective of this study was to compare an electroimmunoassay (EID) for apolipoproteins A-I and A-II with our previously described radioimmunoassay (RIA) (9).…”

Section: Introductionmentioning

confidence: 99%

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Electroimmunoassay and Radioimmunoassay for the Quantitation of High Density Apolipoproteins A-I and A-II

Mordasini¹,

Riesen²

1980

CCLM

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Summary: An electroimmunodiffusion assay (EID) for the quantitative determination of the major apolipoproteins of the human high density lipoprotein fraction, apolipoproteins A-I and A-II, was compared with a solid phase radioimmunoassay (RIA), previously described by us. Data obtained by both methods largely depended on the pretreatment of the sera. Pretreatment consisted either of delipidation with an ether/ethanol mixture or tetramethyl urea, or of denaturation by heating the sera to 52 °C for 3 hours. Delipidation with ether/ethanol produced a decrease of apolipoprotein A-I values in both methods as compared to treatment with tetramethyl urea. In contrast, the ether/ ethanol treatment did not alter apolipoprotein A-II values in the RIA, while in the EID this procedure led to an increase of apolipoprotein A-II values. Heating gave an increase of both apolipoproteins A-I and A-II in both assays, but results obtained by EID were difficult to interpret because of the appearance of indistinct or double rockets. Comparable results by both methods were obtained, when sera were pretreated with tetramethyl urea. Handling and reproducibility seemed to be slightly better with the EID than with the RIA. Vergleich zwischen Elektroimmunoassay und Radioimmunoassay zur Bestimmung der Apolipoproteine A-I und A-IIZusammenfassung: Ein Elektroimmunodiffusionstest (EID) zur quantitativen Bestimmung der häufigsten Apoproteine der menschlichen High Density Lipoprotein Fraktion, Apolipoprotein A-I und A-II, wurde mit dem von uns früher beschriebenen Festphasen-Radioimmunoassay (RIA) verglichen. Die Resultate, welche mit den beiden Methoden erhalten wurden, hingen in hohem Maße von der Vorbehandlung der Seren ab. Die Vorbehandlung bestand entweder aus der Delipidierung mit einem Ether/Ethanol-Gemisch oder mit Tetramethyl-Harnstoff oder aus Erhitzen auf 52 °C während 3 Stunden. Die Delipidierung mit Ether/Ethanol führte bei beiden Methoden zu einer Erniedrigung der Apolipoprotein Arl-Werte gegenüber Werten, welche nach Tetramethyl-Harnstoff-Behandlung erhalten wurden. Die mit RIA erhaltenen Apolipoprotein --Werte erfuhren dagegen durch die Ether/Ethanol-Behandlung keine Änderung, während beim EIA, im Gegensatz zu Apolipoprotein A-I, eine Erhöhung der Apolipoprotein A-H-Werte festgestellt wurde. Das Erhitzen der Seren ergab bei beiden Methoden eine Erhöhung sowohl der Apolipoprotein A-I-wie der Apolipoprotein A-II-Werte, wobei beim EID die Resultate schwer interpretierbar waren, weil undeutliche oder doppelte "Rockets" auftraten. Vergleichbare Resultate bei beiden Methoden wurden erhalten, wenn die Seren mit Tetramethyl-Hanistoff vorbehandelt wurden. Die Handhabung und die Reproduzierbarkeit schienen beim EID besser als beim RIA zu sein. Introductionexert a P rotective effect against coronary heart disease

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Electroimmunoassay and Radioimmunoassay for the Quantitation of High Density Apolipoproteins A-I and A-II

Mordasini¹,

Riesen²

1980

CCLM

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“…Overall, HDL triglycerides were most affected by polyunsaturated f:at ingestion whereas phospholipids showed the least change. We predicted that these changes in saturation of the fatty acyl chains in HDL 26.5°and 180C on the saturated and polyunsaturated diets, respectively.…”

Section: Resultsmentioning

confidence: 98%

Effects of Dietary Polyunsaturated and Saturated Fat on the Properties of High Density Lipoproteins and the Metabolism of Apolipoprotein A-I

Shepherd¹,

Packard²,

Patsch³

et al. 1978

J. Clin. Invest.

446

116

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A B S T R A C T In this study we have investigated, in four normal males the effects of dietary saturated and polyunsaturated fat on the chemical composition and thermotropic properties of human high density lipoproteins (HDL) and have measured the influence of the diets on the metabolism of that fraction of HDL apolipoprotein A-I (apoA-I) that undergoes exchange in vitro and accounts for approximately twothirds of the lipoprotein's apoA-I complement. When compared with the saturated fat diet, the polyunsaturated diet reduced plasma cholesterol (24%, P < 0.01) by affecting the cholesterol content in the very low density lipoprotein (125%, P < 0.02), low density lipoprotein (120%, P < 0.01), and high density lipoprotein fractions (133%, P < 0.01). Plasma triglyceride was also lowered (by 13%, P < 0.01). Furthermore, polyunsaturated fat ingestion caused a significant fall in the palmitate and stearate content of HDL triglyceride (41 and 37%, respectively), cholesteryl esters (29 and 35%), and phospholipids (17 and 9%) with a concomitant increase in the linoleate content of these moieties (157, 28, and 29%, respectively). The polyunsaturated diet also produced reciprocal changes in the percentage protein (19%, P <0.02) and phospholipid (411.5%, P < 0.01) in HDL. These compositional changes were associated with an increase in the microscopic fluidity of the polyunsaturated HDL, although both diets had little effect on the fluidity parameters of HDL at body temperature. Rate zonal ultracentrifugation indicated that the HDLJHDL3 ratio fell by 28% (P < 0.05) on the polyunsaturated fat diet. In addition to the above,

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“…LDL-C was calculated with the Friedewald equation (29). ApoA-I was determined by a modified radioimmunoassay (30,31). ApoB was determined by radioimmunoassay (32), with minor modifications.…”

Section: Plasma Hdl-cholesterol and Other Relevant Variablesmentioning

confidence: 99%

Associations between HDL-cholesterol and polymorphisms in hepatic lipase and lipoprotein lipase genes are modified by dietary fat intake in African American and White adults

et al. 2007

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Polymorphisms in genes involved in HDL-cholesterol (HDL-C) metabolism influence plasma HDL-C concentrations. We examined whether dietary fat intake modified relations between HDL-C and polymorphisms in hepatic lipase (LIPC-514C→T), cholesteryl ester transfer protein (CETP TaqIB), and lipoprotein lipase (LPL S447X) genes. Diet (food frequency questionnaire), plasma lipids, and LIPC, CETP, and LPL genotypes were assessed in ~12,000 White and African American adults. In both races and all genotypes studied, minor allele homozygotes had highest HDL-C concentrations compared to the other genotypes (P <0.001). However, main effects were modified by usual dietary fat intake. In African Americans-women somewhat more strongly than men-LIPC TT homozygotes with fat intake ≥33.2% of energy had ~3-4 mg/dL higher HDL-C concentrations than CC and CT genotypes. In contrast, when fat intake was <33.2% of energy, TT homozygotes had HDL-C concentrations ~3.5 mg/dL greater than those with the CC genotype but not different from those with the CT genotype (P interaction =0.013). In Whites, LPL GG homozygotes had greatest HDL-C at lower total, saturated, and monounsaturated fat intakes but lowest HDL-C at higher intakes of these fats (P interaction ≤0.002). Dietary fat did not modify associations between CETP and HDL-C. In conclusion, these data show that plasma HDL-C differs according to LIPC, LPL, and CETP genotypes. In the case of LIPC and LPL, data suggest dietary fat modifies these relations.

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The Structure of Human High Density Lipoprotein and the Levels of Apolipoprotein A-I in Plasma as Determined by Radioimmunoassay

Cited by 209 publications

References 36 publications

Electroimmunoassay and Radioimmunoassay for the Quantitation of High Density Apolipoproteins A-I and A-II

Electroimmunoassay and Radioimmunoassay for the Quantitation of High Density Apolipoproteins A-I and A-II

Effects of Dietary Polyunsaturated and Saturated Fat on the Properties of High Density Lipoproteins and the Metabolism of Apolipoprotein A-I

Associations between HDL-cholesterol and polymorphisms in hepatic lipase and lipoprotein lipase genes are modified by dietary fat intake in African American and White adults

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