The structure of cloned 3'-terminal RNA region of bovine leukemia virus (BLV)

Abstract

cDNA synthesized on the bovine leukemia virus RNA template has been cloned in the pBR322 Pst I site. Colony hybridization with BLV RNA fragments and oligo (dT) has revealed a clone with cDNA insert containing 660 3'-terminal nucleotides of the BLV genome. The nucleotide sequence of the insert corresponding to U3 and R regions of the long terminal repeats (LTR) of viral genome has been determined. BLV U3, like U3 of other retroviruses, presumably contains promoter. The unusually long R region (about 230 bp), a certain homology with ATLV U3-R and some other structural features allow to group BLV LTR together with ATLV LTR in a separate class of retroviral LTR.

Joint Molecular Modeling and Spectroscopic Studies of DNA Complexes of a Bis(arginyl) Conjugate of a Tricationic Porphyrin Designed to Target the Major Groove

“…To this end, we previously designed and synthesized a bis(arginyl) derivative of a tricationic derivative of H 2 TMPyP-4, (BAP) (10) in order to target the d(GGCGCC) 2 sequence, which is encountered in oncogenes (1)(2)(3)(4)(5), mutational hot spots (6), and in the LTR sequence of the HIV-1 genome (8,9). Our theoretical calculations predicted that BAP should bind by intercalation in the middle of the d(GGCGCC) 2 sequence and that each arginine side-chain would bind in the major groove and form hydrogen-bonding interactions with O 6 /N 7 sites belonging to two successive guanines on each strand, upstream from the intercalation site.…”

“…The palindromic d(GGCGCC) 2 sequence, frequently encountered in oncogenes (1)(2)(3)(4)(5), is also a mutational hot spot in DNA (6), and the recognition sequence of the NarI restriction enzyme (7). It is part of the Primary Binding Site of the Long Terminal Repeat of the HIV-1 retrovirus (8,9), thus a most important target for the design of novel drugs that would selectively recognize and bind it.…”

Molecular Cloning and Sequencing of an Australian Isolate of Proviral Bovine Leukaemia Virus DNA: Comparison with other Isolates

“…Deschamps et al (1981) used cloned proviral DNA to prove the exogenous nature of BLV and since then a number of full and partial length clones have been isolated (Sagata et al, 1983;Tsimanis et al, 1983;Gelmann et al, 1984;Kashmiri et al, 1984;Rice et al, 1984;Derse et al, 1985;Itohara & Sekikawa, 1987). Sagata et al (1983) compared the restriction map of a Japanese isolate with that of the Belgian (Deschamps et al, 1981) and American FLK isolates (Kettmann et al, 1981) and demonstrated that the integrated provirus differed significantly in the various isolates.…”

Theoretical Design of a Bistetrapeptide Derivative of Mitoxantrone Targeted Towards the Double-Stranded Hexanucleotide Sequence d(GGCGCC)2

“…It is also encountered in the genome of retroviruses as well as in oncogenes. Although the following list is by no means exclusive, let us mention that it occurs in the upstream region of the LAY/ AIDS retrovirus, as part of a tRNALys primer binding site, downstream of the Long Terminal Repeat ( 4-7); it occurs twice in the upstream region of a c-one gene, at steps 48-53 and 890-895 (8); once in the U3-R region of Bovine Leukemia Virus eDNA, at steps 240-245 (9); twice in the Retinoblastoma gene promoter, at steps 270-265 and 50-55 (10); twice in the evolutionarily conserved section of the chicken myb proto-oncogene, at a fifteen nucleotide interval, at steps 242-237 and 223-218, and twice more upstream and downstream within a 500 nucleotide interval, at steps 312-307 and 171-176 (11); it is also encountered at the purported junction between a four-stranded guanine oligomer and B-DNA at codon 12 of c-Ha-ras gene (12).…”